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Method for preparing sucrose ester through whole-cell catalysis

A whole-cell catalyst and catalytic preparation technology, applied in biochemical equipment and methods, microorganism-based methods, microorganisms, etc., can solve the problems of difficult product separation and extraction, long growth cycle of fermentation method, low substrate conversion rate, etc., to achieve High operational stability, beneficial to the protection of the environment, and the effect of simplifying the product separation steps

Inactive Publication Date: 2015-08-05
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Compared with the fermentation method, whole-cell catalysis overcomes the shortcomings of the fermentation method, such as long growth cycle, complex metabolites, low substrate conversion rate, difficult product separation and extraction, and high energy consumption.

Method used

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  • Method for preparing sucrose ester through whole-cell catalysis
  • Method for preparing sucrose ester through whole-cell catalysis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 2

[0032] Add 5g / L yeast extract, 5g / L ammonium sulfate, 1g / L dipotassium hydrogen phosphate, 0.2g / L crystalline magnesium sulfate and 5g / L anhydrous glucose in 1L distilled water to induce culture Pseudomonas stutzeri, collected by centrifugation after 48 hours of culture, and freeze-dried whole-cell catalyst; 60 mg of sucrose, 0.06 g (the mass ratio of sucrose to whole-cell catalyst is 1:1) whole-cell catalyst of Pseudomonas stutzeri Cell catalyst, 20 mmoL (1:50 molar ratio of sucrose to acyl donor) vinyl methacrylate and 2 ml tert-amyl alcohol / dimethyl sulfoxide (5:1 volume ratio) dual-solvent medium Put it in a Erlenmeyer flask and vibrate in an air bath constant temperature oscillator at 40 °C and 200 rpm. After 48 h of reaction, the reaction conversion rate is 48.4%.

Embodiment 3

[0034] Add 5g / L yeast extract, 5g / L ammonium sulfate, 1g / L dipotassium hydrogen phosphate, 0.2g / L crystalline magnesium sulfate and 5g / L anhydrous glucose in 1L distilled water to induce culture Pseudomonas stutzeri, collected by centrifugation after 48 hours of culture, and freeze-dried whole-cell catalyst; mix 60 mg sucrose, 0.06 g (the mass ratio of sucrose to whole-cell catalyst is 1:1) Pseudomonas stutzeri Whole-cell catalyst, 20 mmoL (1:50 molar ratio of sucrose to acyl donor) vinyl methacrylate, and 2 ml tert-amyl alcohol / dimethylsulfoxide (5:1 volumetric ratio) dual-solvent medium Placed in a stoppered Erlenmeyer flask, placed in an air bath constant temperature oscillator at 50 °C and 200 rpm, and oscillated after 48 hours of reaction, the reaction conversion rate was 24.9%.

Embodiment 4

[0036] Add 10g / L yeast extract, 10g / L ammonium sulfate, 2g / L dipotassium hydrogen phosphate, 0.4g / L crystalline magnesium sulfate and 10g / L anhydrous glucose in 1L distilled water to induce culture Pseudomonas stutzeri, collected by centrifugation after 48 hours of culture, and freeze-dried whole-cell catalyst; 60 mg of sucrose, 0.06 g (the mass ratio of sucrose to whole-cell catalyst is 1:1) whole-cell catalyst of Pseudomonas stutzeri Cell catalyst, 20 mmoL (1:50 molar ratio of sucrose to acyl donor) vinyl methacrylate and 2 ml tert-amyl alcohol / dimethyl sulfoxide (5:1 volume ratio) dual-solvent medium Put it in a Erlenmeyer flask and vibrate in an air bath constant temperature oscillator at 40 °C and 200 rpm. After 48 h of reaction, the reaction conversion rate was 14.2%.

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Abstract

The invention discloses a method for preparing sucrose ester through whole-cell catalysis. The method includes the steps that yeast extract, ammonium sulfate, dipotassium phosphate, crystal magnesium sulfate and an inductive agent are added into distilled water to serve as culture media so as to conduct induced cultivation on pseudomonas stutzeri, centrifugal separation is carried out after cultivation is carried out, and freeze drying is carried out to prepare a whole-cell catalyst; a tert-amyl alcohol / dimethyl sulfoxide double solvent reaction medium is prepared in a reactor, saccharose, the whole-cell catalyst and vinyl methacrylate are added, and sucrose ester is synthesized after a reaction is made under the ordinary pressure; and high-purity sucrose ester is separated from products with the thin layer chromatography. The sucrose ester is synthesized through whole-cell catalysis, the shortcomings that in the prior art, chemical synthesis is tedious in step, the reaction efficiency is low, the environment is polluted, and the selectivity is low are overcome; the complex step of enzyme extraction needed when synthesis cannot be achieved is avoided, and the method has the advantages of being soft in reaction condition, environmentally friendly, simple and controllable in reaction process and high in product purity, and enabling the products to be easily separated from a reaction mixing system.

Description

technical field [0001] The invention relates to the field of whole cell catalysis and synthesis, in particular to a method for preparing sucrose ester by whole cell catalysis. Background technique [0002] Among polyol-type nonionic surfactants, sugar fatty acid esters and alkyl glucosides are the two most widely used surfactants. Especially sugar fatty acid esters are widely used because of their non-toxicity and non-irritating effect on the skin. It also has many functions such as decontamination, emulsification, washing, dispersion, moistening, diffusion, anti-oxidation, penetration, foaming, viscosity adjustment, sterilization, anti-aging, anti-crystallization and anti-static, etc. It is widely used in daily chemical, food and pharmaceutical industries. has been widely used. [0003] Sucrose fatty acid esters are a large class of organic compounds produced by the reaction of sucrose and fatty acid esters. It can be decomposed into glucose and fatty acids in the human ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/12C12R1/38
Inventor 赵光磊张奔林丹李晓凤
Owner SOUTH CHINA UNIV OF TECH