Plant volatile matter imitation attractant and pesticide thereof

[0054] Example 1

[0055] Nineteen volatiles were isolated and identified from robust tomato shoots by adsorption. Make a hollow glass column with a small opening of 6-10 mm in diameter at both ends, with a volume of 4.5-5.5 liters. The inlet is connected to a compressed air bottle, and the outlet is connected to a Tenax TA adsorption column and a vacuum pump. Before adsorbing volatiles, activate the Tenax TA column with nitrogen at 270-280°C for 4-8 hours, add 190-210 grams of robust tea tips in a glass container, blow air, and at the same time pump air from the back of the adsorption column, at the inlet Flow meters were used to control the flow before and after the Tenax TA column, and the inlet and outlet flow rates were both 300 ml/min.

[0056] After 12 hours, the adsorption column was removed, rinsed with re-distilled ether, and then slowly purged with nitrogen to 20 μL, and 1 μL was injected into GC-MS for detection, and the components were identified with standard samples.

[0057] 19 chemical components in tea shoot volatiles were identified: 3-Carene, limonene, Terpinolene, α-phellandrene, β-water β-phellandrene, Ocimene, Myrcene, Cis-3-Hexenol, Trans-2-Hexenol, Linalool, benzyl alcohol, eucalyptol, Nonanal, Octanal, Trans-2-Hexenal, cis 3 Cis-3-Hexenyl acetate, Methyl salicylate, eugenol, guaiacol.

[0058] Example 2

[0059] The attractant lure cores were prepared as follows to carry out the field experiments of Examples 3 and 4: 1) Using n-hexane as a solvent, 3-carene, limonene, terpinolene, α-phellandrene, β-water were mixed with Ellandrene, ocimene, myrcene, cis-3-hexenol, trans-2-hexenol, linalool, benzyl alcohol, eucalyptol, nonanal, octanal, trans-2-hexenal, Cis-3-hexene acetate, methyl salicylate, eugenol, and guaiacol were formulated into taste sources with a concentration of 0.0001-100 μg/ml, respectively, and the taste sources that had an attracting effect on Bemisia tabaci were respectively The three kinds of taste sources which have the avoidance effect on Bemisia tabaci are combined according to 0.5-1: 0.5-1: 0.5-1.

[0060] 2) Soak the rubber head made of natural rubber with absolute ethanol for more than 24 hours, dry it, and then soak the rubber head with an attractant for 24 hours to make the lure core.

[0061] Example 3 Field Bioassay Experiment

[0062] This experiment was completed in Yucheng Experimental Base of Chinese Academy of Sciences, Nanbeizhuang, Yucheng City, Shandong Province.

[0063] From June to July 2013, the 19 volatile compounds 3-carene, limonene, terpinolene, α-phellandrene, β-phellandrene, basilene, myrcene, cis -3-hexenol, trans-2-hexenol, linalol, benzyl alcohol, eucalyptol, nonanal, octanal, trans-2-hexenal, cis-3-hexene acetate, salicyl Methyl acid, eugenol, guaiacol, prepared with n-hexane as solvent 10 -1 , 10 -3 , 10 -5 , 10 -7 The concentrated solution is used as the lure component. The blank yellow board and the yellow board with n-hexane lure cores were used as controls, a total of 78 different lures, and each treatment was repeated at least 6 times to test the attracting effects of various lures on Bemisia tabaci and greenhouse whitefly.

[0064] Data processing: The significant differences of the mean values ​​were analyzed by spss13.0 One Way ANOVA, and the LSD and Duncan methods were used for multiple comparisons. The data in this paper were expressed by mean ± SD.

[0065] Using n-hexane as solvent and 19 different compounds as solvent to prepare 10 -1 , 10 -3 , 10 -5 , 10 -7 The number of Bemisia tabaci lures induced by the natural rubber slow-release bait core made from the concentrated solution was an average of 134.2±18.0 heads/plate, slightly lower than the blank control 135.8±31.60 heads/plate, and slightly higher than the n-hexane control 131.6 ±26.85 heads/board, but the difference between the three was not significant (F (2,466) =1.016, P=0.365); the number of B. tabaci lured by different concentrations of compounds was significantly different (F (3,72) =9.123,P=0.000), where 10 -5 (142.9±21.4 heads/plate) concentration of lure was significantly greater than concentration of 10 -1 (121.6±7.6 heads/board) and 10 -7 (128.7±9.1 heads/plate) concentration of lure.

[0066] all 10 -1 The attracting effect of the concentration of lure core (121.6±7.6) on B. tabaci was lower than that of blank control (135.8±31.60) and n-hexane control (131.6±26.85) (F (2,124)= 2.295, P = 0.105); the best attracting effect was benzyl alcohol, the number of Bemisia tabaci was 137.9±31.5 heads/board, the worst was cis-3-hexenol, and the The number of lice was 91.8±12.9 heads/plate; 10 -1 The concentration of 3-carene, cis-3-hexenol and methyl salicylate trapped B. tabaci was significantly lower than the blank control and n-hexane control, and the number of whiteflies trapped was 93.9±22.8 , 91.8±12.9 and 98.7±11.9 heads/plate, which were 30.9%, 32.4% and 27.3% lower than the blank control, respectively. 10 -3 The number of Bemisia tabaci (133.1±15.8 heads/plate) attracted by the lure core of the concentration was slightly higher than that of the n-hexane control (131.6±26.85) and slightly lower than that of the blank control (135.8±31.60) (F (2,124) =0.206, P=0.814); the best attracting effect was cis-3-hexenol (165.5±36.5 heads/plate), and the worst was Nonanal (103.2±31.22 heads/plate) ). 10 -5 The attracting effect of the concentration of lure core on B. tabaci was significantly higher than that of n-hexane control and blank control (F (2,124) =4.678, P=0.011); the attracting effect of two alcohols cis-3-hexenol, benzyl alcohol and one aldehyde trans-2-hexenal on B. tabaci was significantly higher than that of the control, and the attracting effect on B. tabaci was significantly higher. The inducement effect was 40.1%, 36.8% and 38.7% higher than the blank control, respectively. 10 -7 There was no significant difference in the attracting effect of the concentration of lure core on B. tabaci compared with the control (F (2,124) = 1.717, P = 0.185), among which benzyl alcohol is the best attracting effect, the number of Bemisia tabaci is 142.6±32.1 heads/board, and the worst is linalool, and the number of Bemisia tabaci is 120.2 ± 7.95 heads (Table 1).

[0067] Table 1. n-Hexane is the solvent, and different compound solutions are formulated into different concentrations of attractants on the number of Bemisia tabaci (head/board)

[0068]

[0069]

[0070] * Indicates that the attracting effect is significantly different from the blank control (Spss13.0, One-Way ANONA, LSD Multiple Comparisons.P<0.05).

[0071] Using n-hexane as solvent and 19 different compounds as solvent to prepare 10 -1 , 10 -3 , 10 -5 , 10 -7 The number of greenhouse whiteflies lured by the natural rubber slow-release lure core made from the concentrated solution was an average of 301.9±39.0 heads/plate, which was slightly lower than that of the blank control (321.4±73.81 heads/plate) and the n-hexane control (313.4 ±75.61 heads/plate), but the difference was not significant (F(2,466)=2.605, P=0.075); the number of greenhouse whiteflies attracted by different concentrations of compounds was significantly different (F(3,72)=8.878, P=0.000 ), where 10 -3 (301.8±22.2 heads/board), 10 -5 (322.7±50.7 heads/board) and 10 -7 (313.8±30.5 heads/plate) concentration of lure was significantly greater than the concentration of 10 -1 (269.2±25.2 head/board) lure core.

[0072] ANOVA showed that 10 -1 The lure effect of the concentration of lure core (269.2±25.2 head/plate) on greenhouse whitefly was significantly lower than that of blank control (321.4±43.81 head/plate) and n-hexane control (313.4±45.61 head/plate) (F (2,124) = 5.775, P = 0.004); 10 -1 Cis-3-Hexenyl acetate has the best lure effect in the lure core with the concentration of 309.7±47.9 heads/board of greenhouse whiteflies. The worst lure effect is methyl salicylate. was 226.8±27.6 heads/plate; the number of cis-3-hexenol, trans-2-hexenal and methyl salicylate was significantly lower than that of the blank control, and the number of greenhouse whiteflies attracted was significantly lower than that of the blank control. They were 27.7%, 28.3% and 29.4% lower than the blank control, respectively. 10 -3 The number of greenhouse whiteflies attracted by the concentration of lure core (301.8±22.2 heads/plate) was lower than that of n-hexane control and blank control (F(2,124)=4.419, P=0.014), but the difference between them was not significant; 10 -3 Among the lure cores with the highest concentration, Nonanal (333.8±35.5 heads/plate) had the best lure effect, and Eugenol (265.2±72.1 heads/plate) had the worst lure effect. 10 -5 The attracting effect of all the lure cores (322.7±50.7 heads/plate) on the greenhouse whitefly was higher than that of the n-hexane control and the blank control, and there was no significant difference between the three (F (2,124) =0.361, P=0.698); the best attracting effect on greenhouse whitefly was cis-3-hexenol (427.8±51.2 heads/plate), and the worst attracting effect was trans-2-hexenal (245.0±51.2 heads/plate). 44.9 heads/plate); of which, 3-carene (421.8±30.5 heads/plate), cis-3-hexenol (427.8±51.2 heads/plate), trans-2-hexenal (413.2±37.9 heads/plate) panel) on the greenhouse whitefly was significantly higher than the control, and the attracting effect on the greenhouse whitefly was 31.2%, 33.3%, and 28.6% higher than that of the blank control, respectively. 10 -7 The lure effect of the concentration of lure core (313.8±30.5 head/plate) on the greenhouse whitefly had no significant difference compared with the n-hexane control and the blank control (F(2,124)=0.463, P=0.630), among which the attracting effect was the best Cis-3-Hexenyl acetate (359.6±50.3 heads/plate) was the most attractive, and methyl salicylate (260.7±37.4 heads/plate) was the least effective in attracting (Table 2).

[0073] Table 2. n-Hexane is the solvent, and different compound solutions are formulated into different concentrations of attractants. The effect of attracting whiteflies on greenhouse whiteflies (head/board)

[0074]

[0075] * Indicates that the attracting effect is significantly different from the blank control (Spss13.0, One-Way ANONA, LSD Multiple Comparisons.P<0.05).