Plant volatile matter imitation attractant and pesticide thereof

A technology of plant volatiles and attractants, which can be used in attracting pests, plant growth regulators, and repellents, and can solve the problems of pesticide residues, natural enemies, and non-target biological damage, and achieve low cost and easy transportation , Ease of use

Inactive Publication Date: 2015-08-12
INST OF ZOOLOGY CHINESE ACAD OF SCI
2 Cites 13 Cited by

AI-Extracted Technical Summary

Problems solved by technology

[0007] In order to overcome the problem of damage and pesticide residues caused by the use of traditional chemical pesticides to natural enemies and non-target organism...
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Method used

This experiment utilizes 3 kinds of compound combinations that have attracting effect to whitefly to find that they can significantly strengthen the attracting effect (table 3) to whitefly, utilize 3 kinds of compound combinations that have evasive effect to whitefly to find that they The combination can significantly enhance the repellent effect on Bemisia tabaci (Table 4). Utilizing the combination of compounds that have an attractive effect on whitefly in the greenhouse at a ...
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Abstract

The invention relates to a plant volatile matter imitation attractant, a relevant pesticide, and an application of the attractant and the pesticide. The plant volatile matter imitation attractant is one or a mixture containing more of 3-carene, cis-3-hexenol, linalool, benzyl alcohol, trans-2-hexenal, methyl salicylate and eugenol. The attractant and the relevant pesticide are mainly used for controlling Bemisia tabaci and/or whitefly, and especially Bemisia tabaci and/or whitefly of tomato in greenhouse; the attractant and the relevant pesticide can be used in field to reduce or completely avoid the use of traditional chemical pesticides, and are suitable for current production of organic vegetables and nuisance-free vegetables; and the attractant and the relevant pesticide have the advantages of high efficiency, stable performances, trapping of many Bemisia tabaci and/or whitefly in an experimental area within 1-2d, convenient transportation, use convenience and low cost.

Application Domain

Technology Topic

Cis-3-hexenolEugenol +10

Image

  • Plant volatile matter imitation attractant and pesticide thereof
  • Plant volatile matter imitation attractant and pesticide thereof
  • Plant volatile matter imitation attractant and pesticide thereof

Examples

  • Experimental program(4)

Example Embodiment

[0054] Example 1
[0055] Nineteen volatiles were isolated and identified from robust tomato shoots by adsorption. Make a hollow glass column with a small opening of 6-10 mm in diameter at both ends, with a volume of 4.5-5.5 liters. The inlet is connected to a compressed air bottle, and the outlet is connected to a Tenax TA adsorption column and a vacuum pump. Before adsorbing volatiles, activate the Tenax TA column with nitrogen at 270-280°C for 4-8 hours, add 190-210 grams of robust tea tips in a glass container, blow air, and at the same time pump air from the back of the adsorption column, at the inlet Flow meters were used to control the flow before and after the Tenax TA column, and the inlet and outlet flow rates were both 300 ml/min.
[0056] After 12 hours, the adsorption column was removed, rinsed with re-distilled ether, and then slowly purged with nitrogen to 20 μL, and 1 μL was injected into GC-MS for detection, and the components were identified with standard samples.
[0057] 19 chemical components in tea shoot volatiles were identified: 3-Carene, limonene, Terpinolene, α-phellandrene, β-water β-phellandrene, Ocimene, Myrcene, Cis-3-Hexenol, Trans-2-Hexenol, Linalool, benzyl alcohol, eucalyptol, Nonanal, Octanal, Trans-2-Hexenal, cis 3 Cis-3-Hexenyl acetate, Methyl salicylate, eugenol, guaiacol.

Example Embodiment

[0058] Example 2
[0059] The attractant lure cores were prepared as follows to carry out the field experiments of Examples 3 and 4: 1) Using n-hexane as a solvent, 3-carene, limonene, terpinolene, α-phellandrene, β-water were mixed with Ellandrene, ocimene, myrcene, cis-3-hexenol, trans-2-hexenol, linalool, benzyl alcohol, eucalyptol, nonanal, octanal, trans-2-hexenal, Cis-3-hexene acetate, methyl salicylate, eugenol, and guaiacol were formulated into taste sources with a concentration of 0.0001-100 μg/ml, respectively, and the taste sources that had an attracting effect on Bemisia tabaci were respectively The three kinds of taste sources which have the avoidance effect on Bemisia tabaci are combined according to 0.5-1: 0.5-1: 0.5-1.
[0060] 2) Soak the rubber head made of natural rubber with absolute ethanol for more than 24 hours, dry it, and then soak the rubber head with an attractant for 24 hours to make the lure core.

Example Embodiment

[0061] Example 3 Field Bioassay Experiment
[0062] This experiment was completed in Yucheng Experimental Base of Chinese Academy of Sciences, Nanbeizhuang, Yucheng City, Shandong Province.
[0063] From June to July 2013, the 19 volatile compounds 3-carene, limonene, terpinolene, α-phellandrene, β-phellandrene, basilene, myrcene, cis -3-hexenol, trans-2-hexenol, linalol, benzyl alcohol, eucalyptol, nonanal, octanal, trans-2-hexenal, cis-3-hexene acetate, salicyl Methyl acid, eugenol, guaiacol, prepared with n-hexane as solvent 10 -1 , 10 -3 , 10 -5 , 10 -7 The concentrated solution is used as the lure component. The blank yellow board and the yellow board with n-hexane lure cores were used as controls, a total of 78 different lures, and each treatment was repeated at least 6 times to test the attracting effects of various lures on Bemisia tabaci and greenhouse whitefly.
[0064] Data processing: The significant differences of the mean values ​​were analyzed by spss13.0 One Way ANOVA, and the LSD and Duncan methods were used for multiple comparisons. The data in this paper were expressed by mean ± SD.
[0065] Using n-hexane as solvent and 19 different compounds as solvent to prepare 10 -1 , 10 -3 , 10 -5 , 10 -7 The number of Bemisia tabaci lures induced by the natural rubber slow-release bait core made from the concentrated solution was an average of 134.2±18.0 heads/plate, slightly lower than the blank control 135.8±31.60 heads/plate, and slightly higher than the n-hexane control 131.6 ±26.85 heads/board, but the difference between the three was not significant (F (2,466) =1.016, P=0.365); the number of B. tabaci lured by different concentrations of compounds was significantly different (F (3,72) =9.123,P=0.000), where 10 -5 (142.9±21.4 heads/plate) concentration of lure was significantly greater than concentration of 10 -1 (121.6±7.6 heads/board) and 10 -7 (128.7±9.1 heads/plate) concentration of lure.
[0066] all 10 -1 The attracting effect of the concentration of lure core (121.6±7.6) on B. tabaci was lower than that of blank control (135.8±31.60) and n-hexane control (131.6±26.85) (F (2,124)= 2.295, P = 0.105); the best attracting effect was benzyl alcohol, the number of Bemisia tabaci was 137.9±31.5 heads/board, the worst was cis-3-hexenol, and the The number of lice was 91.8±12.9 heads/plate; 10 -1 The concentration of 3-carene, cis-3-hexenol and methyl salicylate trapped B. tabaci was significantly lower than the blank control and n-hexane control, and the number of whiteflies trapped was 93.9±22.8 , 91.8±12.9 and 98.7±11.9 heads/plate, which were 30.9%, 32.4% and 27.3% lower than the blank control, respectively. 10 -3 The number of Bemisia tabaci (133.1±15.8 heads/plate) attracted by the lure core of the concentration was slightly higher than that of the n-hexane control (131.6±26.85) and slightly lower than that of the blank control (135.8±31.60) (F (2,124) =0.206, P=0.814); the best attracting effect was cis-3-hexenol (165.5±36.5 heads/plate), and the worst was Nonanal (103.2±31.22 heads/plate) ). 10 -5 The attracting effect of the concentration of lure core on B. tabaci was significantly higher than that of n-hexane control and blank control (F (2,124) =4.678, P=0.011); the attracting effect of two alcohols cis-3-hexenol, benzyl alcohol and one aldehyde trans-2-hexenal on B. tabaci was significantly higher than that of the control, and the attracting effect on B. tabaci was significantly higher. The inducement effect was 40.1%, 36.8% and 38.7% higher than the blank control, respectively. 10 -7 There was no significant difference in the attracting effect of the concentration of lure core on B. tabaci compared with the control (F (2,124) = 1.717, P = 0.185), among which benzyl alcohol is the best attracting effect, the number of Bemisia tabaci is 142.6±32.1 heads/board, and the worst is linalool, and the number of Bemisia tabaci is 120.2 ± 7.95 heads (Table 1).
[0067] Table 1. n-Hexane is the solvent, and different compound solutions are formulated into different concentrations of attractants on the number of Bemisia tabaci (head/board)
[0068]
[0069]
[0070] * Indicates that the attracting effect is significantly different from the blank control (Spss13.0, One-Way ANONA, LSD Multiple Comparisons.P<0.05).
[0071] Using n-hexane as solvent and 19 different compounds as solvent to prepare 10 -1 , 10 -3 , 10 -5 , 10 -7 The number of greenhouse whiteflies lured by the natural rubber slow-release lure core made from the concentrated solution was an average of 301.9±39.0 heads/plate, which was slightly lower than that of the blank control (321.4±73.81 heads/plate) and the n-hexane control (313.4 ±75.61 heads/plate), but the difference was not significant (F(2,466)=2.605, P=0.075); the number of greenhouse whiteflies attracted by different concentrations of compounds was significantly different (F(3,72)=8.878, P=0.000 ), where 10 -3 (301.8±22.2 heads/board), 10 -5 (322.7±50.7 heads/board) and 10 -7 (313.8±30.5 heads/plate) concentration of lure was significantly greater than the concentration of 10 -1 (269.2±25.2 head/board) lure core.
[0072] ANOVA showed that 10 -1 The lure effect of the concentration of lure core (269.2±25.2 head/plate) on greenhouse whitefly was significantly lower than that of blank control (321.4±43.81 head/plate) and n-hexane control (313.4±45.61 head/plate) (F (2,124) = 5.775, P = 0.004); 10 -1 Cis-3-Hexenyl acetate has the best lure effect in the lure core with the concentration of 309.7±47.9 heads/board of greenhouse whiteflies. The worst lure effect is methyl salicylate. was 226.8±27.6 heads/plate; the number of cis-3-hexenol, trans-2-hexenal and methyl salicylate was significantly lower than that of the blank control, and the number of greenhouse whiteflies attracted was significantly lower than that of the blank control. They were 27.7%, 28.3% and 29.4% lower than the blank control, respectively. 10 -3 The number of greenhouse whiteflies attracted by the concentration of lure core (301.8±22.2 heads/plate) was lower than that of n-hexane control and blank control (F(2,124)=4.419, P=0.014), but the difference between them was not significant; 10 -3 Among the lure cores with the highest concentration, Nonanal (333.8±35.5 heads/plate) had the best lure effect, and Eugenol (265.2±72.1 heads/plate) had the worst lure effect. 10 -5 The attracting effect of all the lure cores (322.7±50.7 heads/plate) on the greenhouse whitefly was higher than that of the n-hexane control and the blank control, and there was no significant difference between the three (F (2,124) =0.361, P=0.698); the best attracting effect on greenhouse whitefly was cis-3-hexenol (427.8±51.2 heads/plate), and the worst attracting effect was trans-2-hexenal (245.0±51.2 heads/plate). 44.9 heads/plate); of which, 3-carene (421.8±30.5 heads/plate), cis-3-hexenol (427.8±51.2 heads/plate), trans-2-hexenal (413.2±37.9 heads/plate) panel) on the greenhouse whitefly was significantly higher than the control, and the attracting effect on the greenhouse whitefly was 31.2%, 33.3%, and 28.6% higher than that of the blank control, respectively. 10 -7 The lure effect of the concentration of lure core (313.8±30.5 head/plate) on the greenhouse whitefly had no significant difference compared with the n-hexane control and the blank control (F(2,124)=0.463, P=0.630), among which the attracting effect was the best Cis-3-Hexenyl acetate (359.6±50.3 heads/plate) was the most attractive, and methyl salicylate (260.7±37.4 heads/plate) was the least effective in attracting (Table 2).
[0073] Table 2. n-Hexane is the solvent, and different compound solutions are formulated into different concentrations of attractants. The effect of attracting whiteflies on greenhouse whiteflies (head/board)
[0074]
[0075] * Indicates that the attracting effect is significantly different from the blank control (Spss13.0, One-Way ANONA, LSD Multiple Comparisons.P<0.05).
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

no PUM

Description & Claims & Application Information

We can also present the details of the Description, Claims and Application information to help users get a comprehensive understanding of the technical details of the patent, such as background art, summary of invention, brief description of drawings, description of embodiments, and other original content. On the other hand, users can also determine the specific scope of protection of the technology through the list of claims; as well as understand the changes in the life cycle of the technology with the presentation of the patent timeline. Login to view more.
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Similar technology patents

Classification and recommendation of technical efficacy words

  • Stable performance
  • Improve efficiency

URL lookup method for URL filtering system

InactiveCN101605129AFast searchStable performanceSecuring communicationStorage efficiencyBlack list
Owner:BEIJING INSTITUTE OF TECHNOLOGYGY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products