Plant volatile matter imitation attractant and pesticide thereof
A technology of plant volatiles and attractants, which can be used in attracting pests, plant growth regulators, and repellents, and can solve the problems of pesticide residues, natural enemies, and non-target biological damage, and achieve low cost and easy transportation , Ease of use
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[0054] Example 1
[0055] Nineteen volatiles were isolated and identified from robust tomato shoots by adsorption. Make a hollow glass column with a small opening of 6-10 mm in diameter at both ends, with a volume of 4.5-5.5 liters. The inlet is connected to a compressed air bottle, and the outlet is connected to a Tenax TA adsorption column and a vacuum pump. Before adsorbing volatiles, activate the Tenax TA column with nitrogen at 270-280°C for 4-8 hours, add 190-210 grams of robust tea tips in a glass container, blow air, and at the same time pump air from the back of the adsorption column, at the inlet Flow meters were used to control the flow before and after the Tenax TA column, and the inlet and outlet flow rates were both 300 ml / min.
[0056] After 12 hours, the adsorption column was removed, rinsed with re-distilled ether, and then slowly purged with nitrogen to 20 μL, and 1 μL was injected into GC-MS for detection, and the components were identified with standard sa...
Example Embodiment
[0058] Example 2
[0059] The attractant lure cores were prepared as follows to carry out the field experiments of Examples 3 and 4: 1) Using n-hexane as a solvent, 3-carene, limonene, terpinolene, α-phellandrene, β-water were mixed with Ellandrene, ocimene, myrcene, cis-3-hexenol, trans-2-hexenol, linalool, benzyl alcohol, eucalyptol, nonanal, octanal, trans-2-hexenal, Cis-3-hexene acetate, methyl salicylate, eugenol, and guaiacol were formulated into taste sources with a concentration of 0.0001-100 μg / ml, respectively, and the taste sources that had an attracting effect on Bemisia tabaci were respectively The three kinds of taste sources which have the avoidance effect on Bemisia tabaci are combined according to 0.5-1: 0.5-1: 0.5-1.
[0060] 2) Soak the rubber head made of natural rubber with absolute ethanol for more than 24 hours, dry it, and then soak the rubber head with an attractant for 24 hours to make the lure core.
Example Embodiment
[0061] Example 3 Field Bioassay Experiment
[0062] This experiment was completed in Yucheng Experimental Base of Chinese Academy of Sciences, Nanbeizhuang, Yucheng City, Shandong Province.
[0063] From June to July 2013, the 19 volatile compounds 3-carene, limonene, terpinolene, α-phellandrene, β-phellandrene, basilene, myrcene, cis -3-hexenol, trans-2-hexenol, linalol, benzyl alcohol, eucalyptol, nonanal, octanal, trans-2-hexenal, cis-3-hexene acetate, salicyl Methyl acid, eugenol, guaiacol, prepared with n-hexane as solvent 10 -1 , 10 -3 , 10 -5 , 10 -7 The concentrated solution is used as the lure component. The blank yellow board and the yellow board with n-hexane lure cores were used as controls, a total of 78 different lures, and each treatment was repeated at least 6 times to test the attracting effects of various lures on Bemisia tabaci and greenhouse whitefly.
[0064] Data processing: The significant differences of the mean values were analyzed by spss13.0 ...
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