Silk fibroin anti-adhesion film and preparation method thereof

A silk fibroin and anti-adhesion film technology, applied in the field of medical materials, can solve the problems of insufficient hydrogel strength, lack of anti-adhesion film, and many chemical additives, and achieve good tissue compatibility and blood compatibility , reduce surgical complications, and reduce patient pain

Active Publication Date: 2017-09-26
SUZHOU INST OF NANO TECH & NANO BIONICS CHINESE ACEDEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Due to the fluidity of the liquid during use, it is difficult to achieve a long-term anti-adhesion effect
Chinese invention patent application CN102380121A discloses a medical anti-adhesion material with controllable degradation and its preparation method. The film material is blended with sodium hyaluronate and konjac glucomannan, lactic acid is used as a modification additive, ammonia water and anhydrous The preparation method of the mixed solution of ethanol as a crosslinking agent is complicated, with many chemical additives and toxic side effects
Chinese invention patent application CN102836465A discloses a silk fibroin hyaluronic acid composite gel for injection, with hyaluronate as the main component, adding a chemical cross-linking agent to form a hydrogel, and adding silk fibroin particles to reduce hyaluronic acid The degradation of sodium also has the problem of toxicity residue after chemical crosslinking, and the strength of the hydrogel is not enough to achieve the effect of anti-adhesion film

Method used

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  • Silk fibroin anti-adhesion film and preparation method thereof
  • Silk fibroin anti-adhesion film and preparation method thereof
  • Silk fibroin anti-adhesion film and preparation method thereof

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preparation example Construction

[0034] refer to figure 1 , as above the preparation method of silk fibroin anti-adhesion film comprises steps:

[0035] (a), using domestic silkworm silk as raw material, preparing a silk fibroin solution with a mass concentration of 1-30%;

[0036] (b) preparing a hyaluronate solution with a mass concentration of 0.1-2%;

[0037] (c), preparing a sugar solution with a mass concentration of 1 to 20%;

[0038] (d), heat up the silk fibroin solution prepared in step (a) to 25-35°C in a water bath, then add the hyaluronic acid salt and sugar solution prepared in step (b) and step (c) and stir to mix evenly, then Inject the mixed solution into the mold;

[0039] (e), put the mold injected with the mixed solution in step (d) into a constant temperature and humidity box, dry it for 10-24 hours in an environment with a relative humidity of 60-90% and a temperature of 30-55°C, and remove the mold. The silk fibroin anti-adhesion film is obtained.

[0040] For the effect of anti-ad...

Embodiment 1

[0043] 1. First put 40 grams of Baichang silk into 2 liters of sodium bicarbonate with a mass concentration of 0.2 g / L and 1 g / L sodium dodecyl sulfate (SDS), and treat it at 98-100 ° C for 0.5 hours. Take it out and wash it thoroughly; then put it into 2 liters of sodium bicarbonate with a mass concentration of 0.2g / L and a mixed solution of 1g / L SDS again, treat it at 98-100°C for 0.5 hours, take it out and wash it fully; then put it into a 400mL mass concentration In the 3g / L papain solution, treat at 60°C for 1 hour to degumming the silk, take it out, wash it thoroughly and dry it to obtain pure silk fibroin fiber. Weigh 10g of pure silk fiber and dissolve it in 100mL of lithium bromide solution with a concentration of 9.3mol / L, and slowly stir and dissolve it at a temperature of 60±2°C to form a mixed solution of silk fibroin lithium bromide.

[0044] 2. Put the obtained silk fibroin lithium bromide mixed solution into a dialysis bag, dialyze with deionized water for 3 da...

Embodiment 2

[0052] 1. Put 80 g of raw silk into 4 liters of 0.05% sodium carbonate aqueous solution, treat it at 98-100° C. for 2 hours, degumming the raw silk, take it out and wash it thoroughly, and degumming again to obtain pure silk fibroin. The dried pure silk fibroin is heated and dissolved with 1 L of lithium bromide solution with a concentration of 9.0 mol / L at a temperature of 60±5° C. to obtain a silk fibroin lithium bromide mixed solution.

[0053] 2. Put the obtained silk fibroin lithium bromide mixed solution into a dialysis bag, dialyze with deionized water for 3 days, remove impurities such as lithium bromide, and obtain pure silk fibroin solution; adjust the concentration of silk fibroin protein solution to 20g / L, store Store in a refrigerator at a temperature of 4-8°C for later use.

[0054] 3. Prepare a sodium hyaluronate solution with a mass percentage concentration of 0.5%, and a mannose solution with a mass percentage concentration of 12%, and store them in a refriger...

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Abstract

The invention discloses a silk fibroin anti-adhesion film. The silk fibroin anti-adhesion film contains silk fibroin, hyaluronate and small molecular sugar substances; wherein, calculated by mass percentage, the content of silk fibroin The content of hyaluronate is 60-90%, the content of hyaluronate is 1-10%, and the balance is small molecular carbohydrates. The invention also discloses a preparation method of the silk fibroin anti-adhesion film, comprising steps: (a), using silkworm silk as a raw material, preparing a silk fibroin solution; (b), preparing a hyaluronic acid salt solution; (c), Prepared sugar solution; (d), stir and mix the silk fibroin solution, hyaluronic acid salt and sugar solution evenly, and then inject the mixed solution into the mold; (e), put the mold filled with the mixed solution into a constant temperature and humidity Dry in the oven, remove the mold, and obtain the silk fibroin anti-adhesion film.

Description

technical field [0001] The invention belongs to the technical field of medical materials, and in particular relates to a silk fibroin anti-adhesion film prepared by using silk fibroin as a raw material for isolating different organs after surgery and a preparation method thereof. Background technique [0002] Tissue adhesion is a wound caused by inflammation, friction, surgery, etc. During the treatment process, excessive fibrous tissue is produced or bleeding forms blood clots, which causes adhesion of surrounding organs and tissues. Theoretically speaking, almost all operations involve the problem of adhesion between tissues. There are nearly ten million cases of various types of surgery in the country every year, and almost all of them involve anti-adhesion between tissues and local anti-inflammation. It can not only cause serious complications, such as: adhesive intestinal obstruction after abdominal and pelvic surgery, postoperative epilepsy after craniotomy, secondary...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L31/04A61L31/14
Inventor 卢神州靳健
Owner SUZHOU INST OF NANO TECH & NANO BIONICS CHINESE ACEDEMY OF SCI
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