Solid-state continuous fermentation method of Bacillus subtilis

Abstract

The invention discloses a solid-state continuous fermentation method of Bacillus subtilis, which comprises the following steps: 1) feeding a solid-state fermentation material through a feeding bin, and carrying out high-temperature sterilization in a continuous sterilization device; 2) in a closed purified room, inoculating activated Bacillus subtilis into the sterilized solid-state fermentation material; 3) in the closed purified room, spreading the material obtained in the step 2) on the initial position of a continuous fermentation bed operating at uniform speed, and fermenting for 22-26 hours, wherein the humidity in the purified room is controlled at 80-95%, the purified room is ventilated through a fresh air system, and the fermentation temperature is 37-42 DEG C; and 4) drying the obtained fermentation product in the closed purified room to obtain the dried fermentation product. The method can overcome the defects of low effective viable count and high infectious microbe quantity in the common fermentation process, thereby achieving the goals of enhancing the fermentation viable count, ensuring the product quality and enhancing the use effect.

Description

technical field [0001] The invention relates to a microbial fermentation process-a solid-state continuous fermentation process of bacillus subtilis. Background technique [0002] Bacillus subtilis, an aerobic Gram-positive bacillus, is one of the probiotics officially approved by the Ministry of Agriculture of my country. It has good antibacterial properties, good stability, and no resistance to antibiotics. , non-toxic to animals and other remarkable features, favored by the majority of researchers, and was first applied to aquatic products and achieved remarkable results. [0003] In 1872, Cohn officially named "Bacillus subtilis" (Bacillus subtilis). It is an aerobic, spore-forming rod-shaped bacterium that can produce more than a dozen enzymes such as protease, amylase, cellulase, and glucanase, as well as a variety of enzymes such as subtilisin, polymyxin, and nystatin. Antibiotics, so it has a good role in promoting animal digestion, improving feed utilization and inh...

AI Technical Summary

Problems solved by technology

[0005] ① Ordinary solid-state fermentation process is simple, sterilization is not continuous, not suitable for large-scale production

[0006] ② Rough quality control in the ordinary solid-state fermentation process leads to high content of miscellaneous bacteria in the product. Escherichia coli and Salmonella are easy to exceed the standard, and it is difficult to meet the quality requirements

[0008] ④ Ordinary solid-state fermentation process takes too long to sterilize, which can easily lead to excessive moisture in the product, thus affecting the discharge

[0009] Due to the existence of the above-mentioned problems, it has further influenced the application of Bacillus subtilis in livestock and poultry

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