Application of a Fluorescent Probe in the Detection of Molecular Hydrogen Peroxide

A fluorescent probe, hydrogen peroxide technology, applied in the field of analytical chemistry, can solve the problems of lack of hydrogen peroxide probe, inability to detect hydrogen peroxide, lack of targeting in detection, etc., to achieve broad application prospects, resistance to other molecular interference The effect of strong ability and high coincidence rate
CN104949946BInactive Publication Date: 2017-12-12UNIV OF JINAN
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
UNIV OF JINAN
Publication Date
2017-12-12
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses the application of a fluorescent probe in the detection of hydrogen peroxide molecules. The fluorescent probe is used for sensing and detecting the content of hydrogen peroxide in the water environment and cell lysosomes; the sensing detection includes Fluorescence detection, visual qualitative detection, cell imaging detection. The invention realizes the selective and rapid detection of the H2O2 molecular probe, and has good selectivity and strong anti-interference ability of other molecules. In addition, the change of the color of the solution can be observed with the naked eye, and the change of the fluorescent color can also be observed under the ultraviolet light. It is a fluorescent probe with a color sensing function. This probe can be applied to the detection of hydrogen peroxide in intracellular lysosomes. Compared with commercial lysosomal dyes, it has a high overlap rate for imaging hydrogen peroxide in lysosomes, and the co-localization of the two dyes The coefficient is 0.94.
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Description

technical field

[0001] The invention relates to the application of a fluorescent probe in the detection of hydrogen peroxide molecules, belonging to the technical field of analytical chemistry. Background technique

[0002] Reactive oxygen species (ROS) are some oxygen-containing free radicals (hydroxyl free radicals and superoxide anion free radicals) and non-free radicals (hydrogen peroxide and hypochlorous acid, etc.) that play a very important role in many physiological and pathological processes in organisms. ) collectively. Various ROS are produced by enzymatic and non-enzymatic reactions in organisms under physiological and pathological conditions such as oxidative stress and inflammation. Modern biomedical research shows that the ROS produced in the body is closely related to the occurrence and development of some diseases and the aging of the body. hydrogen peroxide (H 2 o 2 ) As a signaling molecule, it participates in a variety of signal transduction processes...

Claims

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