Nitrogen-doped carbon quantum dot with high fluorescence quantum yield and preparation method and application of nitrogen-doped carbon quantum dot
A fluorescence quantum yield, carbon quantum dot technology, applied in fluorescence/phosphorescence, chemical instruments and methods, preparations for in vivo experiments, etc. Meet the needs of industrial applications, high fluorescence quantum efficiency, good biocompatibility
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[0032] Example 1
[0033] Add 3 g of urea and 1 mL of HOOC-PEG-COOH (Mw 600) to a three-necked flask containing 25 mL of glycerol or diethylene glycol, and water bath at 80 °C until the urea is completely dissolved. Under the protection of inert gas, it was heated to 120 °C and held for 1 h to remove water, then heated to 220 °C and held for 4 h, and then the heating was stopped. The resulting mixture was dialyzed using a dialysis bag with a molecular weight cut-off of 1K for two days, then the product was concentrated by a rotary evaporator, and the resulting viscous brown liquid, namely fluorescent carbon dots, was freeze-dried. Transmission electron microscopy analysis showed that the size of the carbon dots was 2-4 nm, and the nitrogen content was 9 wt% measured by elemental analysis, and showed bright blue fluorescence under the excitation of 365 nm wavelength.
[0034] 1*10 seeds per well of 96-well plate 4 HeLa cells were cultured for 24 h. Different concentrations...
Example Embodiment
[0036] Example 2
[0037] Add 0.1 g of urea and 0.1 mL of HOOC-PEG-COOH (Mw 600) into a three-necked flask containing 5 mL of glycerol or diethylene glycol, and water bath at 80 °C until the urea is completely dissolved. Under the protection of inert gas, it was heated to 120 °C and held for 1 h to remove water, then heated to 200 °C and held for 8 h, and then the heating was stopped. The resulting mixture was dialyzed with a dialysis bag with a molecular weight cut-off of 1K for two days, and then the product was concentrated by a rotary evaporator, and the obtained viscous brown liquid, namely, fluorescent carbon dots, was freeze-dried. Transmission electron microscopy analysis showed that the size of the carbon dots was 2-4 nm, and the nitrogen content was 6 wt% measured by elemental analysis, showing bright blue fluorescence under excitation at 365 nm.
[0038] 1*10 seeds per well of 96-well plate 4 HeLa cells were cultured for 24 h. Different concentrations (6, 12, 2...
Example Embodiment
[0040] Example 3
[0041] Add 5 g of urea and 1 mL of HOOC-PEG-COOH (Mw 600) into a three-necked flask containing 25 mL of glycerol or diethylene glycol, and water bath at 80 °C until the urea is completely dissolved. Under the protection of inert gas, it was heated to 120 °C and held for 1 h to remove water, then heated to 240 °C and held for 1 h, and then the heating was stopped. The resulting mixture was dialyzed with a dialysis bag with a molecular weight cut-off of 1K for two days, and then the product was concentrated by a rotary evaporator, and the obtained viscous brown liquid, namely, fluorescent carbon dots, was freeze-dried. Transmission electron microscopy analysis showed that the size of the carbon dots was 2-4 nm, and the nitrogen content was 10 wt% measured by elemental analysis, and showed bright blue fluorescence under the excitation of 365 nm wavelength.
[0042] 1*10 seeds per well of 96-well plate 4 HeLa cells were cultured for 24 h. Different concentrat...
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