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A kind of preparation method of glycyrrhizin liposome

A technology of licorice polysaccharides and liposomes, which is applied in the direction of liposome delivery, medical preparations of non-active ingredients, pharmaceutical formulas, etc., can solve the problems that the preparation of licorice polysaccharide liposomes does not have too much guiding value, and achieve a solution Adhering to the wall, avoiding the effect of not being easy to dissolve

Active Publication Date: 2017-12-12
NANJING AGRICULTURAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0007] However, because the structure of polysaccharides has its own characteristics, such as the type of monosaccharide, the connection site, the configuration of monosaccharide and glycosidic bonds, and the number of repeating units, etc., will be different due to the different types of polysaccharides extracted. The preparation method of rehmannia glutinosa polysaccharide liposome is formed at the characteristics of polysaccharides in Scrophulariaceae plants, especially rehmannia glutinosa, so even if there are other liposome preparation methods in the prior art, the licorice polysaccharide liposome The preparation of the

Method used

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  • A kind of preparation method of glycyrrhizin liposome
  • A kind of preparation method of glycyrrhizin liposome
  • A kind of preparation method of glycyrrhizin liposome

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Embodiment 1

[0051] Embodiment 1 Ethanol injection method prepares GUPL

[0052] Accurately weigh 0.4g of soybean lecithin, 0.05g of cholesterol and 800.1g of Tween, dissolve it in 10mL of absolute ethanol, and ultrasonically dissolve it fully; -1 The PBS (pH 7.4) solution of licorice polysaccharide was slowly introduced into the former, kept at 45°C and continued to shake for 30 minutes; placed in a 250mL round bottom flask, evaporated under reduced pressure at 50°C to remove ethanol, and then placed in an ultrasonic cell pulverizer for ultrasonic treatment (intensity 40% , open in 2.5s, stop in 2.5s, and act for 15min), so that the glycyrrhizic polysaccharide is fully and evenly integrated into the liposome, and also makes the particle size of the glycyrrhizic polysaccharide liposome more uniform, thereby obtaining the glycyrrhizic polysaccharide liposome suspension Then extrude it respectively through microporous membranes with pore diameters of 0.45 μm and 0.22 μm, respectively repeati...

Embodiment 2

[0054] Embodiment 2 film dispersion method prepares GUPL

[0055] Accurately weigh 0.4g of soybean lecithin, 0.05g of cholesterol and 800.1g of Tween, dissolve it in 10mL of chloroform and methanol (1:1) solution, and ultrasonically dissolve it fully; place it in a 250mL round bottom flask, and evaporate it under reduced pressure at 50°C Organic solvent, when a uniform film is formed on the wall of the flask, introduce 20mL of -1 The PBS (pH 7.0) solution of licorice polysaccharide was evaporated under reduced pressure at 50°C to elute the bottle wall film; hydrated at 25°C for 0.5h; then placed in an ultrasonic cell pulverizer for ultrasonic treatment (intensity 40%, 2.5s on, 2.5 s stop, and act for 15min), the glycyrrhizic polysaccharide is fully and evenly fused in the liposome, and the particle diameter of the glycyrrhizic polysaccharide liposome is also made more uniform simultaneously, thereby obtains the glycyrrhizic polysaccharide liposome suspension; Then it is squeez...

Embodiment 3

[0057] Embodiment 3 Ammonium sulfate gradient method prepares GUPL

[0058] Accurately weigh 0.4g of soybean lecithin, 0.05g of cholesterol and 800.1g of Tween, dissolve it in 10mL of absolute ethanol, and ultrasonically dissolve it fully; under the vibration of a water bath shaker at 50°C, introduce the former into 10mL of 10% ammonium sulfate solution , then placed in a 250mL round-bottomed flask, evaporated under reduced pressure at 50°C to remove the organic solvent; dialyzed in PBS (pH 7.0) for 24h; -1 The PBS (pH 7.0) solution of licorice polysaccharide was slowly introduced into the former, kept at 50°C and continued to shake for 30 minutes; then placed in an ultrasonic cell pulverizer for ultrasonic treatment (intensity 40%, 2.5s on, 2.5s off, 20 minutes of action), so that the licorice polysaccharide Fully and evenly fused into the liposomes, and at the same time make the particle size of the licorice polysaccharide liposomes more uniform, so as to obtain the licorice...

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Abstract

The invention discloses a preparation method of glycyrrhizin liposome. A reverse evaporation method is adopted. The established process conditions are as follows: the mass ratio of soybean phospholipid and cholesterol is 2:1-10:1, and the mass ratio of soybean phospholipid and glycyrrhizin is 2:1-10:1. It is 5:1-25:1, the mass of Tween 80 and soybean lecithin is 1:1-1:20, the water bath temperature is 25℃-65℃, and the ultrasonic time is 5min-25min. The present invention prepares Glycyrrhiza polysaccharide into liposomes for the first time. Through response surface optimization, raw materials can be saved to the greatest extent possible, and a higher drug encapsulation rate (up to 79.12%) can be obtained at the same time. Combined with the action of ultrasound, the prepared liposomes can be More uniform and more stable, its average particle size is 136.4±0.151nm.

Description

technical field [0001] The invention belongs to the technical field of medicine preparation, and in particular relates to a preparation method of licorice polysaccharide liposome. Background technique [0002] Licorice has a long history of medicinal use in my country and is a tonic Chinese herbal medicine. Glycyrrhizapolysaccharide (GP) is one of the main active ingredients of licorice, and it is a kind of α-D-pyranose extracted from licorice. The polysaccharide components are composed of rhamnose, dextran, arabinose and galactose Composed of dextran as the main chain. The polysaccharides, whose basic structure is a single glucan linked by α-(1,4) bonds, have the functions of anti-inflammation, enhancing body immunity, etc., and the morphological structure of the glycyrrhiza polysaccharide molecule is a helical structure image. [0003] Liposomes (liposomes) are artificially synthesized bilayer monolayer phospholipids or concentric microcircular lipid capsules separated b...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K9/127A61K31/715A61K47/24A61P29/00A61P37/04
Inventor 武毅李恩涛李友英胡元亮刘家国王德云牛亚乐高珍珍熊文刘伟新
Owner NANJING AGRICULTURAL UNIVERSITY
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