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Bone defect regeneration and repair tissue-engineered bone, as well as construction method and application thereof

A technology for tissue engineering bone and bone defect, applied in the field of tissue engineering and regenerative medicine medical biomaterials, it can solve the problems that materials cannot be degraded, and the repair effect of bone defect is not ideal, so as to achieve good mechanical properties and avoid the loss of extracellular matrix , preserve the effect of communication and interaction

Active Publication Date: 2015-11-04
广东芙金干细胞再生医学有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

②Allogeneic bone, allogeneic bone grafts can provide osteoconductive scaffolds, and retain most of the osteoinductive protein components, with both osteoconductive and osteoinductive capabilities, but immune rejection can kill donor-derived cells in the grafted bone , so the lack of osteogenic capacity
Its overall performance is that the material cannot or should not be degraded, and its biocompatibility is poor, resulting in almost no ductility after repair, poor thermal conductivity, incomplete integration with the original bone after repair, and differences in shape, size, and thickness from autogenous bone, etc.
From the point of view of the physiological process of bone regeneration, the ability to form bone and the effect on the repair of bone defects using simple bone materials, materials combined with factors, and materials combined with cell technology are not ideal.

Method used

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  • Bone defect regeneration and repair tissue-engineered bone, as well as construction method and application thereof
  • Bone defect regeneration and repair tissue-engineered bone, as well as construction method and application thereof
  • Bone defect regeneration and repair tissue-engineered bone, as well as construction method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0056] Example 1: Repair of Rat Skull Limit Defect

[0057] In this embodiment, rats are used as test organisms, and the limit defect of the skull is repaired, which specifically includes the following steps.

[0058] Step 1. Preparation of culture medium

[0059] In this embodiment, four culture solutions are used, namely culture solution I, culture solution II, culture solution III and culture solution IV. Among them, culture medium I was DMEM culture medium, which was purchased from sigma company. Culture medium II is DMEM medium containing FBS, and the volume fraction of FBS in DMEM medium is 10%.

[0060] Culture medium III was composed of FBS, DMEM medium, vitamin C, 8-prenyl citrusin, licochalcone A and icariin. Wherein, the volume fraction of FBS is 10%, the concentration of vitamin C is 100mg / L, and the concentration of 8-isopentenyl citrusin is 10%. -6 mol / L, the concentration of licochalcone A is 5×10 -5 mol / L, the concentration of icariin is 10 -7 mol / L.

[...

Embodiment 2

[0073] Example 2: Repair of Rabbit Skull Ultimate Defect

[0074] In this embodiment, rabbits are used as test organisms, and the limit defect of the skull is repaired, which specifically includes the following steps.

[0075] Step 1. Preparation of culture medium

[0076] In this embodiment, four culture solutions are used, namely culture solution I, culture solution II, culture solution III and culture solution IV. Wherein, culture solution I and culture solution II are the same as in Example 1.

[0077] Culture medium III was composed of FBS, DMEM medium, vitamin C, 8-prenyl citrusin, licochalcone A and icariin. Wherein, the volume fraction of FBS is 10%, the concentration of vitamin C is 100mg / L, and the concentration of 8-isopentenyl citrusin is 10%. -5 mol / L, the concentration of licochalcone A is 10 -6 mol / L, the concentration of icariin is 10 -6 mol / L.

[0078] Culture medium IV is composed of FBS, DMEM medium, β-sodium glycerol phosphate, dexamethasone, vitamin ...

Embodiment 3

[0090] Embodiment 3: Repair of limit defect of porcine skull,

[0091] In this embodiment, pigs are used as test organisms, and the limit defect of the skull is repaired, which specifically includes the following steps.

[0092] Step 1. Preparation of culture medium

[0093] In this embodiment, four culture solutions are used, namely culture solution I, culture solution II, culture solution III and culture solution IV. Wherein, culture solution I and culture solution II are the same as in embodiment 1 and embodiment 2.

[0094] Culture medium III was composed of FBS, DMEM medium, vitamin C, 8-prenyl citrusin, licochalcone A and icariin. Wherein, the volume fraction of FBS is 10%, the concentration of vitamin C is 100mg / L, and the concentration of 8-isopentenyl citrusin is 10%. -6 mol / L, the concentration of licochalcone A is 10 -5 mol / L, the concentration of icariin is 10 -5 mol / L.

[0095] Culture medium IV is composed of FBS, DMEM medium, β-sodium glycerol phosphate, d...

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Abstract

The invention discloses a bone-defect regeneration and repair tissue-engineered bone, as well as a construction method and an application thereof. The tissue-engineered bone is a composite laminated structural body formed by three layers of mesenchymal stem cell polymers and two layers of bone matrix particles, wherein the composite laminated structural body is a complex formed by mesenchymal stem cell polymers and bone matrix particles which are alternately arranged; the mesenchymal stem cell polymer comprises mesenchymal stem cells and extracellular matrix which is secreted by the mesenchymal stem cells and is distributed around the mesenchymal stem cells. The tissue-engineered bone formed by adopting the construction method can be used for simultaneously compounding living cells and active factors, the repairing and reconstructing process is quick, the joint of new bone and surrounding autogenous bone is complete and uniform in thickness. The tissue-engineered bone is suitable for bone-defect regeneration and repair, physiological regeneration of bones is realized, and the repairing treatment effect of bone defect is improved.

Description

technical field [0001] The invention belongs to the technical field of tissue engineering and regenerative medicine medical biomaterials, in particular to a tissue engineering bone with physiological regenerative bioactivity and a construction method thereof. The tissue engineered bone can be used for regeneration and repair of bone defect. Background technique [0002] In the current field of orthopedic treatment, bone defects of extremities and cranial bones caused by various traumas, infections, tumor resections, and certain congenital developmental deformities are still the most common and thorny problems in orthopedic clinics. Treatment methods for bone defects mainly include bone grafting, bone lengthening, and tissue engineering techniques. [0003] The construction of tissue engineered bone has been developed more and more, and its advantages have become more and more prominent, but the effects of different construction strategies are quite different. Among them, t...

Claims

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Application Information

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IPC IPC(8): A61L27/40A61L27/38A61L27/36A61L27/54A61L27/50
Inventor 梅丽洁
Owner 广东芙金干细胞再生医学有限公司
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