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Application of gamma-aminobutyric acid serving as snakehead feed additive for resisting ammonia nitrogen stress

A technology of aminobutyric acid and anti-ammonia nitrogen, applied in animal feed, animal feed, application, etc., can solve problems such as decreased disease resistance, reduced yield, and impact on large-scale breeding of snakeheads

Inactive Publication Date: 2015-11-11
JILIN AGRICULTURAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It turns out that the snakehead is a ferocious carnivorous fish and eats more live fish, which is a harmful fish for aquaculture. However, it has been able to feed and domesticate it, which solved the bottleneck problem of large-scale farming. The decline in disease resistance of the species leads to a decrease in yield, which affects the process of large-scale farming of snakeheads

Method used

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  • Application of gamma-aminobutyric acid serving as snakehead feed additive for resisting ammonia nitrogen stress
  • Application of gamma-aminobutyric acid serving as snakehead feed additive for resisting ammonia nitrogen stress
  • Application of gamma-aminobutyric acid serving as snakehead feed additive for resisting ammonia nitrogen stress

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] Embodiment 1 snakehead compound feed

[0015] Snakehead compound feed (A), by weight:

[0016] Domestic fish meal 45.50, aspartic acid 0.35, fish oil 1.28, peanut oil 1.48, corn 6.50, soybean meal 8.00, cotton meal 6.60, rapeseed meal 5.80, peanut meal 3.50, vitamin premix 0.95, wheat bran 14.50, subflour 28.50, bentonite 2.55 , alginate 0.50, mineral salt premix 1.80;

[0017] Snakehead compound feed (B), by weight:

[0018] Domestic fish meal 43.50, aspartic acid 0.37, fish oil 1.45, peanut oil 1.79, corn 6.50, soybean meal 8.00, cotton meal 8.60, rapeseed meal 10.80, peanut meal 6.50, vitamin premix 0.95, wheat bran 10.50, subflour 24.50, bentonite 2.55 , alginate 0.50, mineral salt premix 1.80;

[0019] Snakehead compound feed combination (C), by weight:

[0020] Domestic fish meal 45.50, aspartic acid 0.35, fish oil 1.28, peanut oil 1.48, corn 6.50, soybean meal 8.00, cotton meal 6.60, rapeseed meal 5.80, peanut meal 3.50, vitamin premix 0.95, wheat bran 14.50,...

Embodiment 2

[0025] GABA was purchased from Zhejiang Yiwan Biotechnology Co., Ltd. (content 99%). 80g / L high-density cultured snakehead, using the above-mentioned four feeds A, B, C, and D for feeding.

[0026] The breeding experiment was carried out in a room temperature-controlled single-circulation system, and the feeding time was 8 weeks. Healthy and lively snakeheads (body mass: 26.52±0.14g) were selected and randomly assigned to 12 aquariums. They were randomly divided into A, B, C, and D a total of 4 groups, each with 3 repetitions. Feed twice a day (09:00, 16:00), the daily feeding rate is 2.5-4.0% of the body weight. During the test, the water temperature was 23°C-30°C, the pH was 7.1±0.1, the dissolved oxygen was greater than 5.0mg / L, and the ammonia nitrogen was 1.0mg / L.

[0027] Feed 0, 2, 4, 6 and 8w, take samples, and calculate the average weight gain rate, growth dispersion, feed intake rate and protein efficiency.

[0028]

[0029]

Embodiment 3

[0031] After the end of the experiment, food was fasted for 24 hours, the fish were anesthetized, blood was collected from the tail vein, and serum was prepared by centrifugation at 4000r / min at 4°C for 10 minutes. The test fish that had been blooded were dissected immediately, and the hepatopancreas, head kidney and spleen were taken out. All the above operations were carried out on ice, and all samples were stored in a -80°C refrigerator for future use. Serum and tissue total protein (TP), albumin (ALB), urea (UREA), glucose (GLUC), aspartate aminotransferase (AST), and alanine aminotransferase (ALT) were measured.

[0032]

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Abstract

The invention provides snakehead matching feed. The snakehead matching feed comprises, by weight, 43.50-45.50 parts of domestic fish meal, 0.35-0.37 part of aspartic acid, 1.28-1.45 parts of fish oil. 1.48-1.79 parts of peanut oil, 6.4-6.60 parts of corn, 7.90-8.10 parts of bean pulp, 6.60-8.60 parts of cottonseed meal, 5.80-10.80 parts of rapeseed meal, 3.50-6.50 parts of peanut meal, 0.94-0.96 part of vitamin premix, 10.50-14.50 parts of wheat bran, 24.50-28.50 parts of wheat middling, 2.54-2.56 parts of bentonite, 0.4-0.6 part of sodium alginate, 1.70-1.90 parts of mineral salt premix and 1.10-1.30 parts of gamma-aminobutyric acid. It is shown by experiments that growth and metabolism of snakeheads can be promoted, the feed utilization rate is increased, and the acute ammonia nitrogen stress ability is improved.

Description

technical field [0001] The invention belongs to the field of aquaculture, and specifically relates to the use of gamma-aminobutyric acid as a feed additive for snakehead snakeheads against ammonia nitrogen stress, and a compound feed for snakehead snakeheads. Background technique [0002] With the rapid development of intensive aquaculture, the concentration of ammonia nitrogen in the aquaculture water body increases, and the ammonia concentration in hemolymph and other tissues of aquatic animals increases. The toxicity of ammonia is mainly due to the fact that non-ionic ammonia can diffuse freely through the cell membrane, affecting its stability and enzyme activity, and even leading to cell death. Most teleosts are very sensitive to the toxicity of ammonia nitrogen. Excessively high ammonia nitrogen in aquaculture water can reduce the antioxidant capacity of juvenile fish and reduce the activities of SOD and GSH-PX. In recent years, nutritional regulation has been used to...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K1/18A23K1/10A23K1/14A23K1/16A01K61/00A61K31/197
CPCY02A40/81
Inventor 王桂芹郑伟闫先春夏长革付大平刘凡宁丛林梅杨秋实
Owner JILIN AGRICULTURAL UNIV
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