Dryopteris fragrans phloroglucinol derivatives, separation and preparation method for isomers thereof and application thereof
A technology of Phloroglucinol and isomers of Phloroglucinol, applied in the field of chemistry and medicine, to achieve the effect of mild conditions, simple method and good medicinal activity
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Embodiment 1
[0053] The separation and preparation of embodiment 1 phloroglucinol derivatives
[0054] The schematic diagram of the separation and preparation is shown in the appendix figure 1 And attached figure 2 shown.
[0055] S1. Extraction of the total phloroglucinol derivatives of Trichomonas chinensis: extract 10 kg dry coarse powder of Trichomonas 10 times by cold immersion in 50% edible ethanol twice, each time for 24 hours, concentrate and recover under reduced pressure Ethanol to alcohol-free sample aqueous solution;
[0056] S2. Adjust the pH to 1.5-4.5 with hydrochloric acid, let stand for 12-18 hours, and centrifuge to obtain 1.2 kg of precipitated extract.
[0057] S3. After dissolving the precipitated extract sample obtained in S2, mix the sample with silica gel, perform preliminary separation on the silica gel column, and successively use different proportions of petroleum ether: acetone to elute (100:1-1:1) to obtain the corresponding components Fr.A~E.
[0058] Th...
Embodiment 2
[0062] The separation and preparation of embodiment 2 phloroglucinol derivatives
[0063] The schematic diagram of the separation and preparation is shown in the appendix figure 1 And attached figure 2 shown.
[0064] S1. Extraction of the total phloroglucinol derivatives of Trichomonas chinensis: extract 10 kg dry coarse powder of Trichomonas 10 times by cold immersion in 50% edible ethanol twice, each time for 24 hours, concentrate and recover under reduced pressure Ethanol to alcohol-free sample aqueous solution;
[0065] S2. Adjust the pH to 1.5-4.5 with hydrochloric acid, let stand for 12-18 hours, and centrifuge to obtain 1.2 kg of precipitated extract.
[0066] S3. After dissolving the precipitated extract sample obtained in S2, mix the sample with silica gel, perform preliminary separation on the silica gel column, and successively use different proportions of petroleum ether: acetone to elute (100:1-1:1) to obtain the corresponding components Fr. A-E.
[0067] S...
Embodiment 3
[0069] Example 3 In vitro anti-superficial fungus effect of Phloroglucinol 4 pairs of isomer compounds
[0070] (1) Trichophyton rubrum (CMCC(f)T1b) and Microsporum gypsumoidus (CMCC(F)M2C), provided by the Institute of Dermatology, Chinese Academy of Medical Sciences. The microdilution method of dermatophytes was carried out according to the M38-A2 protocol formulated by CLSI in the United States, which is briefly described as follows. Gently scrape the colonies on the surface of the SDA medium with an inoculation loop and grind the mycelium with a sterile grinder, suspend the dermatophyte in sterile saline, adjust the turbidity to 0.5 McFarland turbidity, and count the number of spores with a hemocytometer and short mycelia. Make the bacterial concentration 1×10 3 CFU / ml to 3×10 3 CFU / ml. That is, the 0.5 McFarland turbidity bacteria solution was diluted 1000 times with RPMI-1640 liquid medium, and counted by the hemocytometer to obtain the inoculum solution. Use RPMI-1...
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