Method for pretreating non-lumber fibrous materials by utilizing basic salt in combination with hydrogen peroxide

A non-wood fiber, alkaline salt technology, applied in biosynthesis, biofuel, fermentation, etc., to reduce the pressure on the environment and energy, and create economic value

Inactive Publication Date: 2015-11-18
CHANGZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Bioethanol is currently produced primarily from starch and sugars, so there has been considerable debate about its sustainability

Method used

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  • Method for pretreating non-lumber fibrous materials by utilizing basic salt in combination with hydrogen peroxide
  • Method for pretreating non-lumber fibrous materials by utilizing basic salt in combination with hydrogen peroxide
  • Method for pretreating non-lumber fibrous materials by utilizing basic salt in combination with hydrogen peroxide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Embodiment 1: take bamboo shoot skin as raw material pretreatment and enzymatic hydrolysis method.

[0039] Wash and air-dry the spring bamboo shoots, crush them to less than 5mm for later use. Cellulase (SpezymeCP, enzyme activity 110FPU / mL), β-glucosidase (Novozyme188, 603CBU / mL) and xylanase (MultifectXylanase, 1600FXU / mL), were purchased from Sigma (St.Louis, MO), Provided by Genencor Enzyme Company, USA. Use acetic acid-sodium acetate buffer (pH4.8) to make cellulase, xylanase and β-glucosidase at an enzyme activity ratio of 1FPU:1FXU:2CBU to prepare a mixed enzyme solution, and the enzyme activity of the mixed enzyme solution is Filter paper enzyme activity calculation.

[0040] Pretreatment process:

[0041] Weigh 1g of air-dried spring bamboo shoots and put them into a round bottom flask, add 20mL mixed solvent (i.e. substrate concentration 5g / L), wherein the mixed solvent is 1%H 2 o 2 and 1, 3, 5, 7, 9, 11, 15 and 20% Na 3 PO 4, stirred and reacted at ...

Embodiment 2

[0045] Embodiment 2: Pretreatment method and enzymatic hydrolysis method using corn stalks as raw materials.

[0046] The corn stalks are washed and air-dried, crushed to a size less than 5 mm for later use. Mixed enzyme liquid is identical with embodiment 1.

[0047] Weigh 1g of air-dried corn stalks into a round bottom flask, add 5mL of mixed solvent (i.e. substrate concentration 20g / L), the mixed solvent is 10%H 2 o 2 and 20%Na 3 PO 4 , stirred and reacted at 120° C. for 1 h, after the reaction was completed, add 2 times the volume of deionized water and stirred at room temperature for 30 min to regenerate the cellulose raw material. Then filter and wash, and put the solid residue in a sealed plastic bag for later use. The enzymatic hydrolysis process is the same as in Example 1, wherein the compound enzyme is 30 FPU / (g substrate). After the above pretreatment and enzymatic hydrolysis, the final reducing sugar yield and glucose yield were 70.71% and 51.01%, respecti...

Embodiment 3

[0048] Embodiment 3: the pretreatment method and the enzymatic hydrolysis method using spring bamboo skin as raw material.

[0049] Wash and air-dry the spring bamboo shoots, crush them to less than 5mm for later use. Mixed enzyme liquid is identical with embodiment 1.

[0050] Weigh 1g of air-dried spring bamboo shoot skin into a round bottom flask, add 10mL of mixed solvent (i.e. substrate concentration 10g / L), wherein the mixed solvent is 5% H 2 o 2 and 9%Na 3 PO 4 , stirred and reacted at 50° C. for 6 hours, after the reaction was completed, an equal volume of deionized water was added and stirred at room temperature for 30 minutes to regenerate the cellulose raw material. Then filter and wash, and put the solid residue in a sealed plastic bag for later use. The enzymatic hydrolysis process is the same as in Example 1, wherein the compound enzyme is 30 FPU / (g substrate). Through the above pretreatment and enzymatic hydrolysis, the final reducing sugar yield and glu...

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Abstract

The invention discloses a method for pretreating non-lumber fibrous materials by utilizing basic salt in combination with hydrogen peroxide and belongs to the technical field of renewable energy. The method comprises the following steps: soaking the non-lumber fibrous materials in a mixed solvent, causing the substrate concentration to be 5-20 g / L, performing treatment for 1-6 h at 50-120 DEG C; after the reaction is over, adding deionized water of 0.5-2-time volume to stir regenerated cellulose materials for 10-30 min at room temperature, and using water to wash solid residue, so that the pretreatment process is finished. The recovery rate of pretreated solids is 40-80%, the lignin removal rate is 20-90%, the cellulose retention rate is 75-95%, and the hemicellulose retention rate is 70-90%. According to the pretreating method, lignin is effectively removed, plenty of cellulose and hemicelluloses are reserved, and accordingly enzymatic hydrolysis saccharification efficiency is improved.

Description

technical field [0001] The invention relates to a method for pretreating non-wood fiber raw materials with alkaline salt combined with hydrogen peroxide, and belongs to the technical field of renewable energy. Background technique [0002] The problems of energy security and greenhouse gas emissions make the use of non-petroleum resources as an alternative energy source increasingly attracting the attention of countries all over the world. Biomass energy is a suitable environmentally friendly and renewable alternative energy source. Bioethanol is currently produced primarily from starch and sugars, so there has been considerable debate about its sustainability. In this case, lignocellulosic biomass has become a noteworthy choice to replace starch ethanol because of its advantages of not competing with grain for land and low price. The production of cellulosic ethanol from lignocellulosic raw materials has been widely recognized. However, due to the limited wood resources ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/10C12P7/64
CPCY02E50/10Y02P20/59
Inventor 卿青周琳琳黄美子张跃何玉财王利群
Owner CHANGZHOU UNIV
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