Biological weedicide granules and preparation method therefor
A herbicide and biological technology, applied in the direction of herbicides and algicides, botanical equipment and methods, biocides, etc., can solve the problem of high actual use per unit area in the field, hinder the practical application value of technology, and increase technical products Cost and other issues, to achieve the effect of promoting microbial activity and crop root development, eliminating air pollution, and increasing porosity
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Embodiment 1
[0033](1) Preparation of Sclerotinia rosenbergii mycelium: inoculate Sclerotinia rosenbergii SC64 in culture medium at 28°C for 5 days, the medium contains 200 g of potatoes, 20 g of glucose, Agar 16g. Sterilize the liquid medium by high-pressure steam for 25 minutes, inoculate 10 cakes of the above-mentioned activated Sclerotinia rosenbergii SC64 bacteria cake, culture at 120 rpm for 5 days at 28°C, centrifuge at 9000 rpm for 18 minutes in a high-speed centrifuge, pour the supernatant, and the precipitate is ready. It is the mycelia of Sclerotia rosenbergii SC64, and the effective concentration of Sclerotia rosei is 1.5×10 based on the mass of mycelium mass in the mycelium. 5 cfu / g≤bacterial concentration≤2.86×10 5 cfu / g.
[0034] (2) Preparation of the solid matrix: the solid matrix was pulverized in a pulverizer, passed through a 20-mesh sieve, and sterilized by conventional high-pressure steam for 25 minutes.
[0035] (3) There are two granulation methods: one is to mix...
Embodiment 2
[0037] Example 2 Effects of different diameters on the pathogenicity of solid particles
[0038] Granulate according to the method shown in Example 1, dry the solid particles under sterile conditions, and take particles with different diameters: X<12 mesh, 12 mesh≤X<8 mesh, 8 mesh≤X<6 mesh, 6 mesh≤X< 2 mesh, X ≥ 2 mesh, do pathogenicity test [pathogenicity test is carried out at 30°C, culture herbicide granules in the center of mature Eupatorium adenophorum leaves (5-6 true leaves from top to bottom) The diameter of necrotic lesions induced after 72 hours was used as an index. The herbicide granules are placed in the center of the Eupatorium adenophorum leaves in a 9cm petri dish with moistened double-layer filter paper in advance, with 2 leaves per dish, and Parafilm (American National Can, Greenwich, CT, USA) is used for sealing, and the petri dish is placed in 30°C, 12hL / D light incubator. Each test treatment was repeated 4 times, and the sterilized solid substrate was se...
Embodiment 3
[0043] The influence of embodiment 3 auxiliary agent content on particle molding rate
[0044] Each handles solid substrate 30g, changes the consumption of white carbon black or glutinous rice flour, granulates according to the first method shown in embodiment 1 (white carbon black or glutinous rice flour consumption are different), weighs after drying, calculates molding rate (will The ratio of the weight of the particles whose diameter is between 1.40-3.35mm after drying to the total weight of the particles).
[0045] Table 2 The effect of different glutinous rice flour and silica content on the particle forming rate
[0046]
[0047] Note: Different lowercase letters after the data in the same column indicate significant differences (P<0.05)
[0048] The results show that: when the amount of glutinous rice flour is 3g-12g, the forming rate is above 91.31%, but the forming rate is obviously lower when the amount of glutinous rice flour is 1g. When the amount of white ca...
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