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Biological weedicide granules and preparation method therefor

A herbicide and biological technology, applied in the direction of herbicides and algicides, botanical equipment and methods, biocides, etc., can solve the problem of high actual use per unit area in the field, hinder the practical application value of technology, and increase technical products Cost and other issues, to achieve the effect of promoting microbial activity and crop root development, eliminating air pollution, and increasing porosity

Active Publication Date: 2015-11-25
NANJING AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In the early stage, we have developed the technology of direct solid fermentation of crop straw and waste to produce solid particles containing mycelia. However, the production cycle of solid fermentation is long and generally takes 7-10 days or even longer. Due to the loose growth of mycelium, the solid particles Inhomogeneity leads to high actual use per unit area in the field, which increases the cost of technical products. In addition, mycelium floats on the surface, and the storage period of products is significantly shortened. Not using commercialization also hinders the practical application value of the technology

Method used

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  • Biological weedicide granules and preparation method therefor
  • Biological weedicide granules and preparation method therefor
  • Biological weedicide granules and preparation method therefor

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033](1) Preparation of Sclerotinia rosenbergii mycelium: inoculate Sclerotinia rosenbergii SC64 in culture medium at 28°C for 5 days, the medium contains 200 g of potatoes, 20 g of glucose, Agar 16g. Sterilize the liquid medium by high-pressure steam for 25 minutes, inoculate 10 cakes of the above-mentioned activated Sclerotinia rosenbergii SC64 bacteria cake, culture at 120 rpm for 5 days at 28°C, centrifuge at 9000 rpm for 18 minutes in a high-speed centrifuge, pour the supernatant, and the precipitate is ready. It is the mycelia of Sclerotia rosenbergii SC64, and the effective concentration of Sclerotia rosei is 1.5×10 based on the mass of mycelium mass in the mycelium. 5 cfu / g≤bacterial concentration≤2.86×10 5 cfu / g.

[0034] (2) Preparation of the solid matrix: the solid matrix was pulverized in a pulverizer, passed through a 20-mesh sieve, and sterilized by conventional high-pressure steam for 25 minutes.

[0035] (3) There are two granulation methods: one is to mix...

Embodiment 2

[0037] Example 2 Effects of different diameters on the pathogenicity of solid particles

[0038] Granulate according to the method shown in Example 1, dry the solid particles under sterile conditions, and take particles with different diameters: X<12 mesh, 12 mesh≤X<8 mesh, 8 mesh≤X<6 mesh, 6 mesh≤X< 2 mesh, X ≥ 2 mesh, do pathogenicity test [pathogenicity test is carried out at 30°C, culture herbicide granules in the center of mature Eupatorium adenophorum leaves (5-6 true leaves from top to bottom) The diameter of necrotic lesions induced after 72 hours was used as an index. The herbicide granules are placed in the center of the Eupatorium adenophorum leaves in a 9cm petri dish with moistened double-layer filter paper in advance, with 2 leaves per dish, and Parafilm (American National Can, Greenwich, CT, USA) is used for sealing, and the petri dish is placed in 30°C, 12hL / D light incubator. Each test treatment was repeated 4 times, and the sterilized solid substrate was se...

Embodiment 3

[0043] The influence of embodiment 3 auxiliary agent content on particle molding rate

[0044] Each handles solid substrate 30g, changes the consumption of white carbon black or glutinous rice flour, granulates according to the first method shown in embodiment 1 (white carbon black or glutinous rice flour consumption are different), weighs after drying, calculates molding rate (will The ratio of the weight of the particles whose diameter is between 1.40-3.35mm after drying to the total weight of the particles).

[0045] Table 2 The effect of different glutinous rice flour and silica content on the particle forming rate

[0046]

[0047] Note: Different lowercase letters after the data in the same column indicate significant differences (P<0.05)

[0048] The results show that: when the amount of glutinous rice flour is 3g-12g, the forming rate is above 91.31%, but the forming rate is obviously lower when the amount of glutinous rice flour is 1g. When the amount of white ca...

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Abstract

The invention discloses solid granules of biological weedicide Jun Kekuo and a preparation method therefor. The solid granules are prepared through granulating a solid matrix, Sclerotium rolfsii mycelial masses, a binder and water which serve as raw materials; the weight ratio of the solid matrix to the Sclerotium rolfsii mycelial masses to the binder to the water is 30: (35-55): (0.4-12): (12-16); and the solid matrix is any one or more selected from rice straws, wheat straws, rape straws, cotton stalks, Solidago canadensis straws, cane straws, soybean straws, rice hulls, cotton seed hulls, garden deadwood and wood dust, and the binder is selected from white carbon black or glutinous rice flour. According to the solid granules and the preparation method therefor, mycelia are produced by using a liquid fermentation technology, so that the time consumed is shortened to more than half of the time for solid fermentation. A solid powdered material is coated with microbial pesticide active mycelia so as to form a granular formulation, thus, the storage period is prolonged, the drug effect is improved, and the consumption is reduced.

Description

technical field [0001] The invention belongs to the field of herbicide preparations, and relates to a solid particle of a biological herbicide juncoclad and a preparation method thereof. Background technique [0002] Bacteria is a biological herbicide based on Sclerotinia rosenbergii SC64, and its main biological active ingredient is the hyphae of SC64. The fungus was isolated from the pathogenic plant of Solidago canadensis by researchers from the Weed Research Office of Nanjing Agricultural University and applied for a patent. Studies have shown that most of the broad-leaved grasses of the fungus have strong pathogenicity, are safe to major crops such as rice, corn, wheat, and lawn grass, are easy to industrialized production, and have independent intellectual property rights. Therefore, it has a good prospect of being developed into a commercial biological herbicide. [0003] Biological pesticides have a short residual effect in the environment, are easy to decompose, a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N65/44A01N25/12A01P13/00A01N63/04
Inventor 强胜李玲燕张雅丽庄超宋小玲
Owner NANJING AGRICULTURAL UNIVERSITY
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