Technique for activating lignite humic acid by using bioenzyme

A humic acid and bio-enzyme technology, applied in the field of bio-enzyme application, can solve the problems of difficult industrialization of cost, low extraction rate, high energy consumption, etc., and achieve the effect of reducing energy consumption, reducing production cost, and improving efficiency

Active Publication Date: 2015-11-25
JINAN HANGCHEN BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Commonly used methods include caustic alkali dry / wet production process, ammonia water / ammonium bicarbonate activation process, strong acid oxidation hydrolysis process, sulfuric acid acetone extraction process, etc., but the above methods all have problems such as huge energy consumption, serious pollution, and low extraction rate ( The amount of alkali used in the traditional causticization method reaches 15%-40%; the temperature is 90-120°C, and the extraction time is 8-10 hours; the calculation amount of the strong acid oxidation method reaches 15%-40%; the temperature is 85-95°C, and the extraction time is 4- 8 hours), making it difficult to promote its production in industrialization due to factors such as environmental protection and cost

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Utilize biological enzyme to activate lignite humic acid process, comprise the following steps:

[0027] (1) Add 25 parts of lignite humic acid to the buffer;

[0028] (2) Add 10 parts of compound enzyme preparation to the buffer solution in step (1) to obtain a mixed solution;

[0029] (3) Adjust the temperature of the mixed solution to 50°C and stir for 2 hours;

[0030] (4) Add 6 parts of ion exchanger to the mixed solution in step (3), adjust the pH to 9, and raise the temperature to 75°C for 1 hour of ion exchange;

[0031] (5) The centrifuge is a disc centrifuge with a centrifugation speed of 4000rpm for 5min to obtain activated humic acid, and the above-mentioned parts refer to parts by weight.

[0032] The buffer solution is: glycine-hydrochloric acid buffer solution, disodium hydrogen phosphate-citric acid buffer solution in a ratio of 1:1, and the concentration of the buffer solution is 0.25mol / L;

[0033] The compound enzyme preparation is acid α-amylase, ...

Embodiment 2

[0042] Utilize biological enzyme to activate lignite humic acid process, comprise the following steps:

[0043] (1) Add 30 parts of lignite humic acid to the buffer;

[0044] (2) Add 15 parts of compound enzyme preparation to the buffer solution in step (1) to obtain a mixed solution;

[0045] (3) Adjust the temperature of the mixture to 50°C and stir for 2 hours;

[0046] (4) Add 8 parts of ion exchanger to the mixed solution in step (3), adjust the pH to 10, and raise the temperature to 80°C for 2.5 hours of ion exchange;

[0047] (5) The centrifuge is a disc centrifuge with a centrifugation speed of 5000rpm for 5min to obtain activated humic acid, and the above-mentioned parts refer to parts by weight.

[0048] The above buffer solution is: Tris-hydrochloric acid buffer solution, acetic acid-sodium acetate buffer solution in a ratio of 3:1, and the concentration of the buffer solution is 0.08mol / L;

[0049] Above-mentioned compound enzyme preparation is acid chitinase, aci...

Embodiment 3

[0057] Utilize biological enzyme to activate lignite humic acid process, comprise the following steps:

[0058] (1) Add 20 parts of lignite humic acid to the buffer;

[0059] (2) Add 8 parts of compound enzyme preparation to the buffer solution in step (1) to obtain a mixed solution;

[0060] (3) Adjust the temperature of the mixed solution to 37°C and stir for 2 hours;

[0061] (4) Add 4 parts of ion exchanger to the mixed solution in step (3), adjust the pH to 8.5, and raise the temperature to 70°C for 2.5 hours of ion exchange;

[0062] (5) The centrifuge is a disc centrifuge with a centrifugation speed of 3500rpm for 5min to obtain activated humic acid, and the above-mentioned parts refer to parts by weight.

[0063] The above buffer solution is: Tris-hydrochloric acid buffer solution, acetic acid-sodium acetate buffer solution in a ratio of 4:1, and the concentration of the buffer solution is 0.08mol / L;

[0064] The above-mentioned compound enzyme preparation is alkali...

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Abstract

The invention belongs to the technical field of bioenzyme application, and particularly relates to a novel technique for activating lignite humic acid by using a bioenzyme. The technique comprises the following steps: adding lignite into a buffer solution; adding a composite microbe solution and a composite enzyme preparation to obtain a mixed solution; heating the mixed solution, and stirring; adding an ion exchanger, regulating the pH value, heating and carrying out ion exchange; and carrying out centrifugal separation. By using the composite microbes to decompose and activate the humic acid, the temperature, pH value and time are respectively lower than those of the traditional chemical extraction technique, and the yellowish-brown humic acid yield is 35% or above and is much higher than that of the acid-alkali activation process under the same production cost. The technique provided by the invention has obvious cost advantage, and can obviously enhance the economic benefit of the production enterprise.

Description

technical field [0001] The invention belongs to the technical field of biological enzyme applications, and in particular relates to a process for activating lignite humic acid by using biological enzymes. Background technique [0002] Humic acid is an amorphous polymer compound formed by the remains of animals and plants through a series of chemical processes caused by the decomposition and transformation of microorganisms and the geological changes of the earth for hundreds of millions of years. It is widely distributed in soil, weathered coal, lignite, carbon in rock. Humic acid is mainly composed of several elements such as carbon, hydrogen, oxygen, nitrogen, and sulfur, and contains various functional groups such as hydroxyl, carboxyl, quinone, carbonyl, and methoxy groups. It is weakly acidic, cation-exchangeable, and chelating It is a good soil conditioner, which can promote the absorption of fertilizer nutrients and improve fertilizer efficiency when applied togeth...

Claims

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Application Information

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IPC IPC(8): C05G3/04C05F11/02C05G3/80
Inventor 李昆仑赵林晋以杰孙欣
Owner JINAN HANGCHEN BIOTECHNOLOGY CO LTD
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