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A kind of process of preparing γ-aminobutyric acid from quinoa

A technology of aminobutyric acid and quinoa, applied in the field of preparation of gamma-aminobutyric acid, can solve the problems of low safety, few chemical components, harmful to human body and the like

Active Publication Date: 2017-06-16
宝健(北京)生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The chemical synthesis method is mainly realized by the substituting hydrolysis reaction of γ-chlorobutadiene. Potassium phthalimide and γ-chlorobutane cyanide are reacted at 180 degrees Celsius, and then the product is refluxed with concentrated sulfuric acid and purified by crystallization. , the efficiency of synthesizing γ-aminobutyric acid is extremely high, but the reaction conditions are severe, the selected reagents are more dangerous, and the safety is lower. The related operation process of completely removing the chemical components introduced in the production process has not been successfully explored; the microbial synthesis method Mainly use glutamic acid or its derivative sodium glutamate as raw material, and use yeast, lactic acid bacteria, aspergillus and other food-safe microorganisms to ferment. The whole preparation process is always in a complex microbial system, which is difficult to control accurately The products in the whole fermentation and metabolism process may produce metabolites harmful to the human body, and there are certain safety problems; relatively speaking, the plant enrichment method is simple to operate, introduces fewer chemical components, and has a single reaction system, so it has higher safety sex
[0004] The current plant enrichment method is mainly based on germinated brown rice, most of which combine salt ion stress to increase the content of γ-aminobutyric acid in germinated brown rice, thereby introducing metal ions, resulting in excessive heavy metals in the corresponding products

Method used

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  • A kind of process of preparing γ-aminobutyric acid from quinoa
  • A kind of process of preparing γ-aminobutyric acid from quinoa

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] A kind of technique that prepares gamma-aminobutyric acid from quinoa, comprises following specific steps:

[0042] The first step is to select 1000g of high-quality quinoa (calcium content higher than 0.19%), and wash it with 0.2% sodium bisulfite.

[0043] In the second step, add 1500mL ultrapure water and soak for 12 hours under sterile conditions at 30°C.

[0044] Step 3: Drain the water, cover with 3 layers of wet gauze, and germinate for 25 hours under sterile hypoxic conditions at 40°C (continuously add water, and keep the gauze in a moist state).

[0045] Step 4: Dry at 70°C for 4.5 hours, pulverize it, and pass through an 80-mesh sieve.

[0046] Step 5, seal and store under sterile conditions at 4°C until use.

[0047] In step 6, extract with 75% ethanol as a solvent for 3.5 hours, pass through a 0.45 μm microporous membrane, and perform a rotary evaporation operation on the filtrate to only the initial 15%.

[0048] In step 7, put the powder after low-tempe...

Embodiment 2

[0051] A kind of technique that prepares gamma-aminobutyric acid from quinoa, comprises following specific steps:

[0052] The first step is to select 1000g of high-quality quinoa (calcium content higher than 0.14%), and wash it with 0.1% sodium bisulfite.

[0053] In the second step, add 1300mL ultrapure water and soak for 11 hours under sterile conditions at 30°C.

[0054] Step 3: Drain the water, cover with 3 layers of wet gauze, and germinate for 25 hours under sterile hypoxic conditions at 35°C (continuously add water, and keep the gauze in a moist state).

[0055] Step 4: Dry at 70°C for 3.5 hours, crush it, and pass through a 70-mesh sieve.

[0056] Step 5, seal and store under sterile conditions at 4°C until use.

[0057] In step 6, extract with 70% ethanol as a solvent for 3.5 hours, pass through a 0.45 μm microporous membrane, and perform a rotary evaporation operation on the filtrate to only the initial 13%.

[0058] Step 7, put the freeze-dried powder into the e...

Embodiment 3

[0061] A kind of technique that prepares gamma-aminobutyric acid from quinoa, comprises following specific steps:

[0062] Step 1: Select 1000g of high-quality quinoa (calcium content higher than 0.14%), wash it with 0.4% sodium bisulfite.

[0063] In the second step, add 1500mL ultrapure water and soak for 12 hours under sterile conditions at 35°C.

[0064] Step 3: Drain the water, cover with 3 layers of wet gauze, and germinate for 28 hours under sterile hypoxic conditions at 40°C (continuously add water, and keep the gauze in a moist state).

[0065] Step 4: Dry at 75°C for 4.5 hours, crush it, and pass through a 80-mesh sieve.

[0066] Step 5, seal and store under sterile conditions at 4°C until use.

[0067] In step 6, extract with 75% ethanol as a solvent for 4.5 hours, pass through a 0.45 μm microporous membrane, and perform a rotary evaporation operation on the filtrate to only the initial 15%.

[0068] In step 7, put the powder after low-temperature drying into the...

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Abstract

The invention discloses a technology for preparing gamma-aminobutyric acid from quinoa. The technology comprises the following steps: selecting high-grade quinoa as a raw material, and washing the quinoa with a sodium sulfite solution; performing germination treatment on washed quinoa under the conditions of low temperature and low oxygen; drying and smashing germinated quinoa, so as to obtain quinoa powder; performing rough extraction on the quinoa powder by adopting ethyl alcohol as a solvent to obtain a rough extraction product, and extracting the gamma-aminobutyric acid in the rough extraction product by adopting a supercritical extraction method, so as to obtain a final extractive. According to the invention, the technology for preparing gamma-aminobutyric acid from quinoa is provided on the basis of three links of raw material selection and pretreatment, germination enrichment, and gamma-aminobutyric acid extraction, the technology is simple in operation, the yield is high, and the safety of the gamma-aminobutyric acid prepared by the technology is higher than the safety of the gamma-aminobutyric acid prepared by a biosynthesis method and a chemical synthesis method, so that the technology is suitable for industrialized production of gamma-aminobutyric acid.

Description

technical field [0001] The invention relates to the field of preparation of gamma-aminobutyric acid. More specifically, it relates to a process for preparing gamma-aminobutyric acid from quinoa. Background technique [0002] With the development and progress of society, people's living standards and income levels have been greatly improved, and problems such as tension and pressure caused by factors such as work, study, and life have gradually increased, and they have become common among social groups. In a state of high pressure and anxiety for a long time, people will fall into a sub-health state, and the most typical phenomena are insomnia and dreaminess. As a non-protein natural active amino acid, γ-aminobutyric acid has the effects of relieving stress, relaxing the mood, and improving brain vitality. It has a certain relieving effect on anxiety, insomnia, and dreaminess. It has been widely used abroad. [0003] At present, the researches on GABA by scholars in differe...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07C229/08C07C227/40
CPCY02P20/54
Inventor 袁立军马跃
Owner 宝健(北京)生物技术有限公司