Polysaccharide hemostat and preparation method thereof

A technology of hemostatic powder and polysaccharide, applied in the field of biomedicine, can solve the problems of increasing wound infection rate, wound necrosis, inflammatory reaction, etc., and achieve good effect of hemostasis

Inactive Publication Date: 2015-12-23
GUANGDONG TAIBAO MEDICAL SCI TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] These hemostatic materials have certain deficiencies: gelatin collagen material comes from cowhide, which is prone to allergies due to species differences and increases the infection rate of wounds, especially for polluted wounds, so the application is limited; From the blood, it may cause virus infection; the structure of oxidized regenerated cellulose is loose, the adhesion to the bleeding point is poor, it may cause rebleeding, and the hemostasis time is longer, the effect on the rapid bleeding is poor, so it is not suitable as a wound alone Therefore, the application of hemostatic materials is limited; porous zeolite and potato starch will release a lot of heat after absorbing the water in the blood, leading to local necrosis of the wound, inflammation, etc.
Therefore, most chitosan hemostatic materials in the prior art use medium-viscosity, medium-low deacetylation chitosan or high-viscosity medium-low deacetylation chitosan as raw materials, and few use high-deacetylation chitosan as the shell material. Raw material of polysaccharide hemostatic material

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] S1: Add 3g of high guluronic acid sodium alginate with G / M=7:3 to 100ml of distilled water to prepare a 0.03g / ml sodium alginate solution, and make 1g of N-deacetylation degree 90% Add 100ml of 2% dilute acetic acid solution into 100ml of 2% dilute acetic acid solution to prepare a 0.01g / ml chitosan solution. Add the above-mentioned chitosan solution into the sodium alginate solution, and stir evenly to form a stable colloidal solution, namely mixed solution A.

[0026] S2: 300ml of liquid paraffin and 30ml of polysorbate-80 were mixed to obtain oil phase solution B.

[0027] S3: Zinc chloride and calcium chloride are mixed and dissolved in an appropriate amount of distilled water to obtain a solute mass concentration of 2% cross-linking agent C, wherein the mass ratio of zinc chloride to calcium chloride is 1.5:1.

[0028] S4: Add the mixed solution A prepared in step S1 to the oil phase solution B prepared in step S2, stir for 5-10 minutes or until uniform, add 80ml ...

Embodiment 2

[0031] S1: Add 1g of high guluronic acid sodium alginate with G / M=7:3 to 100ml of distilled water to prepare a 0.01g / ml sodium alginate solution, and make 1g of N-deacetylation degree 93% Add 100ml of 2% dilute acetic acid solution into 100ml of 2% dilute acetic acid solution to prepare a 0.01g / ml chitosan solution. Add the above-mentioned chitosan solution into the sodium alginate solution, and stir evenly to form a stable colloidal solution, namely mixed solution A.

[0032] S2: 200ml of liquid paraffin and 20ml of polysorbate-80 were mixed to obtain oil phase solution B.

[0033] S3: Zinc chloride and calcium chloride are mixed and dissolved in an appropriate amount of distilled water to obtain a solute mass concentration of 2% crosslinking agent C, wherein the mass ratio of zinc chloride to calcium chloride is 1:1.

[0034] S4: Add the mixed solution A prepared in step S1 to the oil phase solution B prepared in step S2, stir for 5-10 minutes or until uniform, add 80ml of ...

Embodiment 3

[0037] S1: Add 3g of high guluronic acid sodium alginate with G / M=27:3 to 100ml of distilled water to prepare a 0.03g / ml sodium alginate solution, and 3g of N-deacetylation degree is 96% Add 100ml of 2% dilute acetic acid solution into 100ml of 2% dilute acetic acid solution to prepare a 0.01g / ml chitosan solution. Add the above-mentioned chitosan solution into the sodium alginate solution, and stir evenly to form a stable colloidal solution, namely mixed solution A.

[0038] S2: 300ml of liquid paraffin and 20ml of polysorbate-80 were mixed to obtain oil phase solution B.

[0039] S3: Zinc chloride and calcium chloride are mixed and dissolved in an appropriate amount of distilled water to obtain a solute mass concentration of 2% crosslinking agent C, wherein the mass ratio of zinc chloride to calcium chloride is 1:1.

[0040]S4: Add the mixed solution A prepared in step S1 into the oil phase solution B prepared in step S2, stir for 5-10 minutes or until uniform, then add 80 ...

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Abstract

The invention relates to the field of bio-medicine techniques, and particularly relates to a polysaccharide hemostat and a preparation method thereof. The preparation method comprises the following steps: mixing high-viscosity high-deacetylation-degree chitosan with high-guluronic-acid-content (G) sodium alginate, then adding zinc chloride and calcium chloride into the obtained mixture, carrying out mixed coagulating bath on the obtained object, and stirring and crosslinking the obtained product, so that a polysaccharide hemostat is obtained. The invention also provides components of the polysaccharide hemostat and content thereof. According to the invention, high-viscosity high-deacetylation-degree chitosan and high-G-content (G) sodium alginate are taken as main components of the polysaccharide hemostat, so that obtained high-viscosity high-deacetylation-degree chitosan / zinc-calcium alginate composite microspheres are good in hemostatic effect.

Description

technical field [0001] The invention relates to the technical field of biomedicine, in particular to a polysaccharide hemostatic powder and a preparation method thereof. Background technique [0002] Hemorrhage is one of the causes of death in trauma patients, and rapid and effective control of hemorrhage can significantly reduce the mortality rate. Simple surgery cannot completely control the bleeding, and the use of hemostatic materials can greatly reduce the amount of bleeding. At present, the local hemostatic materials widely used clinically mainly include powders (such as zeolite, collagen powder, and potato starch), porous sponges (gelatin sponge, collagen sponge), oxidized regenerated cellulose, and fibrin glue (such as instant gauze). ), hemostatic gauze, etc. [0003] These hemostatic materials have certain deficiencies: gelatin collagen material comes from cowhide, which is prone to allergies due to species differences and increases the infection rate of wounds, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L15/28A61L15/40A61L15/26A61L26/00
Inventor 韦加娜陈锦涛吴帅何立彬
Owner GUANGDONG TAIBAO MEDICAL SCI TECH
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