Moringa seed micro-molecular peptide extracted from moringa seeds, and extraction method thereof

A technology of small molecular peptides and Moringa seeds, which is applied in the field of polypeptide preparation, can solve the problems of difficult use of small molecular active peptides of polypeptides, the inability to increase the composition of polypeptides as much as possible, and low Baume degree, so as to achieve the effect of safe preparation and use

Inactive Publication Date: 2016-01-13
潘爱国
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Then there are many methods for preparing small molecular peptides in the prior art, for example, CN101194733A, CN101532044A, CN102090680A, and CN1280834A all disclose preparation methods about polypeptides, but the method is single and the extraction performance is not high. Another example, CN101120724A discloses a A kind of microbial fermentation is used to make the polypeptide preparation for feed, which is composed of the following raw materials in weight percentage: Bacillus subtilis 40-55, Lactobacillus plantarum 25-35, Lactobacillus casei 10-35
However, when the prepared polypeptide preparation is used to extract polypeptides from bones, the extraction efficiency is not high, the biological fermentation agent is single, and the application occasions are also limited.
CN102488073A discloses a method for extracting sea cucumber peptides, using enzymatic hydrolysis method, adding sea cucumber special enzyme, enzymatic hydrolysis for 6 hours, stirring for 2 minutes every hour during the enzymatic hydrolysis process; adding activated carbon for deodorization, and then adding sodium hydroxide to adjust pH , during the concentration process, the Baume degree is determined to be controlled at 10. This method cannot ex

Method used

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Effect test

Embodiment 1

[0025] The preparation method of small molecule peptides of Moringa oleifera comprises the following steps in turn: (1) Preparation of starter: use the liquid culture of beneficial bacterial strains to prepare starter, weigh three kinds of proteases, papain, alkaline protease and trypsin, specifically papain 10 parts by weight, 10 parts by weight of alkaline protease, 5 parts by weight of trypsin, and 15 parts by weight of wort, mixed together, and placed in an Erlenmeyer flask filled with culture medium for compound culture, the culture medium is Nutrient agar medium, the temperature for culturing in the Erlenmeyer flask is 37°C, cultivated at 200rpm / min for 20h, so that the concentration of bacteria or spores reaches 1×10 12 Individuals / liter (CFU / L) to obtain starter; (2) Moringa seed pretreatment: fresh Moringa seed is crushed, cleaned, and in a cooking tank, adopting a temperature of 150° C., a pressure of 0.17 MPa and high-pressure cooking for 400 min , the bone dregs ar...

Embodiment 2

[0028] The preparation method of small molecule peptides of Moringa oleifera comprises the following steps in turn: (1) Preparation of starter: use the liquid culture of beneficial bacterial strains to prepare starter, weigh three kinds of proteases, papain, alkaline protease and trypsin, specifically papain 10 parts by weight, 10 parts by weight of alkaline protease, 5 parts by weight of trypsin, and 15 parts by weight of wort, mixed together, and placed in an Erlenmeyer flask filled with culture medium for compound culture, the culture medium is Nutrient agar medium, the temperature for cultivating in the Erlenmeyer flask is 37°C, cultivated at 220rpm / min for 19h, so that the concentration of bacteria or spores reaches 1×10 12 Individuals / liter (CFU / L) to obtain starter; (2) Moringa seed pretreatment: fresh Moringa seed is crushed, cleaned, and in a cooking tank, adopting a temperature of 140° C., a pressure of 0.17 MPa and high-pressure cooking for 350 min , the bone dregs ...

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Abstract

The invention relates to a moringa seed micro-molecular peptide extracted from moringa seeds, and an extraction method thereof. A fermentation, electrolytic and enzymatic hydrolysis comprehensive technology is adopted, and a fermentation agent comprises papain, alkaline proteinase and trypsin according to a mass ratio of 2:2:1. The proportion of moringa seed micro-molecular peptides with the molecular amount of 0-480 dalton in the moringa seed micro-molecular peptide disclosed in the invention is not lower than 87.5%. The moringa seed micro-molecular peptide can be used as a diagnosis peptide, a peptide vaccine, an antibiotic active peptide, a cytokine mimic peptide, an antiviral peptide or an antitumor peptide, and a peptide health product.

Description

technical field [0001] The invention relates to the technical field of polypeptide preparation, in particular to a small molecular peptide of moringa oleifera extracted from moringa oleifera and an extraction method thereof. Background technique [0002] Amino acid molecules are small and proteins are large. Two or more amino acids are dehydrated and condensed to form several peptide bonds to form a peptide, and multiple peptides are folded in multiple stages to form a protein molecule. Dipeptide, referred to as dipeptide, is a protein fragment composed of two amino acids. Hundreds of peptides have been found in organisms, which are essential participants for the body to complete various complex physiological activities. All cells can synthesize polypeptide substances, and their functional activities are also regulated by polypeptides. It involves the fields of hormones, nerves, cell growth and reproduction, and its importance lies in the regulation of various system organ...

Claims

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Application Information

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IPC IPC(8): C12P21/00C12P21/06A23L33/00A61K38/01A61P31/00A61P31/12A61P35/00
Inventor 潘爱国
Owner 潘爱国
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