Method for determination of 2-isopropyl thioxanthone reaction intermediate content
A technology of isopropylthioxanthone and a determination method, which is applied in the field of chemistry, can solve the problems of no public detection method, irritating ammonia smell, and difficulty in controlling the input amount, and achieves stable and reliable results, analysis time period, quality Guaranteed effect
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Embodiment 1
[0023] Example 1 : Sample preparation
[0024] Wash the 10ml vials and syringes, dry them in an oven at 110°C for 2 hours, take them out immediately before use, plug them with rubber stoppers, and keep them cool for later use. Use a syringe to take the reaction intermediate 2-thiochlorobenzoyl chloride solution, which contains about 0.15g of acid chloride per 1ml, 0.73mmol in a vial, and then take 0.5ml of anhydrous n-butylamine (0.36g, 5mmol) In the vial, the syringe was drawn repeatedly 3 times, placed in an ultrasonic oscillator under normal pressure to react for 0.5 h, and the resulting reaction mixture was added to methanol until it was completely dissolved.
Embodiment 2
[0026] Instruments and conditions: Agilent 1200 liquid chromatography system, the chromatographic column adopts octadecylsilane bonded silica gel column; the ultraviolet detection wavelength is 254nm, methanol is the mobile phase, and the flow rate is 1.0ml / min.
[0027] Experimental procedure: take 50ul of the sample solution prepared in Example 1 and add it to the sample tube, add 3ml of methanol to the sample tube, shake it fully, filter out insoluble impurities, then take 10ul of the sample solution and carry out liquid chromatography analysis according to the above conditions, Record the chromatogram, see the attached results figure 1 .
[0028] attached figure 1 It shows that under this chromatographic condition, it can be completely separated, and the retention time of 2-butyl-1,2-benzisothiazolin-3-one, an intermediate derivative of 2-chlorothiobenzoyl chloride, is 3.04min.
Embodiment 3
[0030] Get another batch of reaction intermediate 2-thiochlorobenzoyl chloride solution, analyze according to the mode of embodiment 1 and example 2, the results are shown in the attached figure 2 .
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