Artificial skin with high activity

A technology of artificial skin and surfactant, applied in the field of medical assistance, can solve problems such as affecting the curative effect, and achieve the effect of saving equipment, strong practicability, and thick film layer

Active Publication Date: 2016-03-09
青岛西凯生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in the treatment process of the artificial skin made by several technological schemes in the prior art, due to the limitations of its own structure, it is often impossible to create an environment suitable for the growth of the skin and body for the skin wound, thereby affecting the curative effect.

Method used

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  • Artificial skin with high activity

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] The acquisition of embodiment 1 polypeptide

[0016] A preparation method for polypeptide tissue extraction, comprising the following steps:

[0017] 1) Take the skin of seal carcass, mince it, dissolve it in PBS, add papain and trypsin to treat it for 4 hours, then place it in acidified ethanol at 4°C for digestion for 16 hours, then centrifuge it, take the supernatant and spin it to concentrate, freeze-dry to obtain frozen dry matter;

[0018] 2) Separation by size exclusion chromatography: the freeze-dried product obtained in step 1) is placed in acetic acid with a concentration of 2mol / L for treatment, then loaded into a Sephadex G50 chromatographic column, and balanced with acetic acid with a concentration of 2mol / L , the column flow rate is 10mL / h, 60 components of the chromatographic peak are collected, and freeze-dried to obtain the polypeptide;

[0019] The acidified ethanol is prepared by ethanol and hydrochloric acid with a concentration of 0.7mol / L in a ra...

Embodiment 2

[0030] Embodiment 2 Manufacturing method of artificial skin containing polypeptide

[0031] A method for manufacturing artificial skin, using the following proportioning raw materials in parts by weight: 120 nano-chitosan, 5 polypeptides, 5 lactic acid, 75 organic acids, 30 surfactants, and poly Acrylic acid 60, deionized water 950; And manufacture according to the following steps: the first step, the preparation of A material: get deionized water 950 weight parts, organic acid 75 weight parts, absolute viscosity is the polyacrylic acid 60 weight of 4764mpa.s Parts, placed in a reaction kettle, dissolved at 50°C and 3000 rpm under stirring for 1.5 hours to become material A with a pH value of 3.5-4.0 and 1085 parts by weight; the second step, the preparation of material B: material A After making, keep the temperature of material A in the reactor at 50°C, and stir it at 3000 rpm, and gradually add 120 parts by weight of nano-chitosan into material A in 30 minutes until the nan...

Embodiment 3

[0032] The activity and function detection of embodiment 3 skin

[0033] (1) Skin surface moisture loss test

[0034]Take the artificial skin prepared in Example 2, each cut into a circle with a diameter of 5 cm, and soak in 10% glycerin aqueous solution (glycerin is a commonly used humectant). Then remove and apply to human skin for 30 minutes. Take off the artificial skin, and after another 15 minutes, use the CK Electronic multifunctional skin detector, MPA580 (Cutometer), to detect the water loss on the skin surface. The above steps were also carried out on the skin at the same place without any substance applied, which was used as the control group (A). The results show that after applying the artificial skin without adding the polypeptide of Example 2 to the skin, the water loss rate of the skin at this place is reduced by 18.7%, while the water loss rate of the artificial skin added with the polypeptide prepared in Example 2 is reduced by about About 80%. In other w...

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Abstract

The invention discloses an artificial skin and a manufacturing method thereof. The artificial skin has the characteristics of good surface tension, plump and elastic texture, and bacterium inhibiting performance, and the tissue of the artificial skin is a polymer of nano chitosan and polypeptide. The artificial skin has the advantages of good biomedical characteristics, high medical value, short technical procedure, simple operation, less equipment, and reduced cost. The artificial skin has a good application prospect and application value.

Description

technical field [0001] The invention relates to the technical field of medical assistance, in particular to an artificial skin for skin implantation. Background technique [0002] Artificial skin has a wide range of medical application prospects in the treatment of burns and scalds, so it has received extensive attention and in-depth research from scholars at home and abroad. The skin of burn patients faces a series of problems such as interstitial fluid exudation and infection (especially Pseudomonas aeruginosa infection) during the recovery process. At present, high water vapor permeability films are often used as dressings clinically, including Tegaderm of 3M Company in the United States, OpSite in the United Kingdom, etc. These dressings contain polyurethane plus acrylic adhesives. These adhesives firmly adhere to the body hair and are easy to remove. Cause pain, discomfort and even injury; and these dressings cannot effectively solve problems such as interstitial fluid...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/22A61L27/16A61L27/20A61L27/54A61L27/60C07K7/08
CPCA61L27/16A61L27/20A61L27/227A61L27/54A61L27/60A61L2300/252A61L2300/404A61L2300/416A61L2430/34C07K7/08C08L5/08C08L33/02
Inventor 顾银凤
Owner 青岛西凯生物技术有限公司
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