Preparation method for graphene oxide-TiO2-hypocrellin A three-component compound system and application thereof in photodynamic therapy

A technology of hypocrellin A and ternary compounding, which is applied in the field of photosensitizers, can solve the problems of poor water solubility, inability to realize tumor treatment, difficulties, etc., and achieve the effects of simple preparation method, enhanced visible light response ability, and improved system activity

Inactive Publication Date: 2016-03-23
NANJING NORMAL UNIVERSITY
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Problems solved by technology

[0005] The purpose of the present invention is to overcome the poor water solubility of HA, the inability to be administered by intravenous injection and insufficient photosensitivity in the application of photodynamic therapy, and develop graphene oxide-TiO 2 - Hypocretin A t

Method used

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  • Preparation method for graphene oxide-TiO2-hypocrellin A three-component compound system and application thereof in photodynamic therapy
  • Preparation method for graphene oxide-TiO2-hypocrellin A three-component compound system and application thereof in photodynamic therapy
  • Preparation method for graphene oxide-TiO2-hypocrellin A three-component compound system and application thereof in photodynamic therapy

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Embodiment 1

[0027] A kind of graphene oxide-TiO 2 -The preparation method of Hypocretin A ternary composite system, the steps are as follows:

[0028] 1) Under the condition of vigorous stirring, use a strong oxidizing agent (such as H 2 SO 4 ,KMnO 4 ,NaNO 3 , and H 2 O 2 )oxidised graphite. The sample uses Millipore containing 3% hydrochloric acid TM After washing in water, a negatively charged GO aqueous solution was obtained, which was rich in -OH and -COOH on the surface.

[0029] 2) Stir 15 mL of methanol, 100 μL of anhydrous acetic acid and 200 μL of tetrabutyl titanate evenly, add distilled water dropwise to start the hydrolysis process, and the reaction lasts for 20 h. The product was dialyzed against water for 24 hours using a 12-14KDa dialysis bag to obtain positively charged TiO on the surface 2 Aqueous solution of nanoparticles.

[0030] 3) HA (DMSO solubilization) and step 1) prepared GO in Millipore TMMix in water, shake at constant temperature (20°C) for 24 hours...

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Abstract

The invention relates to a preparation method for graphene oxide-TiO2-hypocrellin A three-component compound system and application thereof in photodynamic therapy. The preparation method for the three-component compound system comprises the steps of firstly, oxidizing graphite through strong oxidant and then washing the oxidized graphite through hydrochloric acid to obtain an aqueous solution of graphene oxide with an electronegative surface; secondly, obtaining an aqueous solution of surface positively charged TiO2 through a sol-gel method; thirdly, achieving effective compounding of the hypocrellin A and the graphene oxide through pi-pi interaction and intermolecular hydrogen bonds between the hypocrellin A and the graphene oxide, and achieving effective compounding of TiO2, the hypocrellin A and the graphene oxide in the aqueous phase through the electrostatic interaction between TiO2 and graphene oxide-hypocrellin A. According to the preparation method, the medicine carrying system is established at the room temperature, so that influence of the high temperature on activity of medicine is prevented; the obtained nanometer compound system is good in dispersity of aqueous phase, has a multiple sensitization mechanism, can improve the active oxygen generation capacity of hypocrellin A remarkably and is low in dark toxity and remarkably improved in phototoxicity compared with hypocrellin A.

Description

technical field [0001] The invention belongs to the technical field of photosensitizers with photodynamic activity, and relates to a graphene oxide-TiO with high active oxygen generation capacity 2 - A method for preparing a ternary composite system of hypocrellin A, and the application of the composite system prepared by the method in photosensitization therapy. Background technique [0002] Photodynamic therapy (PDT) is a new type of tumor treatment. The principle of PDT is that the photosensitizer enters the human body through intravenous injection, and selectively illuminates the tumor tissue to stimulate the photochemical reaction of the photosensitizer retained in the tumor tissue, generating reactive oxygen species (mainly singlet oxygen) to induce irreversible damage to the tumor. Hypocrellin A (hypocrellinA, referred to as HA) is the first natural phototherapy drug extracted from bamboo red fungus and bamboo yellow in my country. It has the advantages of low dark t...

Claims

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Application Information

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IPC IPC(8): A61K41/00A61K47/48A61P35/00
CPCA61K9/0019A61K9/19A61K41/0057
Inventor 周林周家宏魏少华丁怡陈恩懿
Owner NANJING NORMAL UNIVERSITY
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