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A kind of tissue engineered osteochondral composite scaffold and preparation method thereof

A technology of tissue engineering bone and composite scaffold, applied in tissue regeneration, medical science, prosthesis, etc., to achieve good biocompatibility, enhanced mechanical properties, biocompatibility and degradable effects

Inactive Publication Date: 2018-04-24
SOUTHWEST JIAOTONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It can effectively solve the problem of tissue engineering osteochondral composite scaffold preparation

Method used

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  • A kind of tissue engineered osteochondral composite scaffold and preparation method thereof
  • A kind of tissue engineered osteochondral composite scaffold and preparation method thereof
  • A kind of tissue engineered osteochondral composite scaffold and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Accurately weigh 1.00g of polyε-caprolactone, add it into 5mL of chloroform, and prepare a 20w / v% solution, stir and dissolve evenly; accurately weigh 2.00g of polyethylene glycol, add 50ng of BMP-2 into 5mL of distilled water, and prepare into a 40w / v% solution, magnetically stirred to dissolve evenly. Inject into 5mL dry syringes respectively, the syringes are fixed in the card slot of the micro-injection pump, and the output end of the high-voltage DC power supply is fixed on the coaxial nozzle (the inner diameter of the outer needle is 1.1mm, the outer diameter of the inner needle is 0.6mm, and the inner diameter of the inner needle is 0.3 mm), the outer axis is polyε-caprolactone solution, and the flow rate is set at 0.5mL / h. The inner axis is polyethylene glycol solution, the flow rate is set to 0.1mL / h, and the DC high voltage is applied to 20kV. The high-speed rotating drum is used as the receiver (the speed is 1200r / min), and the distance between the coaxial no...

Embodiment 2

[0049] Accurately weigh 1.00g of polyε-caprolactone, add it into 5mL of chloroform, and prepare a 20w / v% solution, stir and dissolve evenly; accurately weigh 2.00g of polyethylene glycol, add 50ng of BMP-2 into 5mL of distilled water, and prepare into a 40w / v% solution, magnetically stirred to dissolve evenly. Inject into 5mL dry syringes respectively, the syringes are fixed in the card slot of the micro-injection pump, and the output end of the high-voltage DC power supply is fixed on the coaxial nozzle (the inner diameter of the outer needle is 1.1mm, the outer diameter of the inner needle is 0.6mm, and the inner diameter of the inner needle is 0.3 mm), the outer axis is polyε-caprolactone solution, and the flow rate is set to 0.5mL / h; the inner axis is polyethylene glycol solution, the flow rate is set to 0.1mL / h, and the DC high voltage is 20kV, which is rotated at a high speed. The roller is used as a receiver (rotating speed is 1200r / min), and the distance between the co...

Embodiment 3

[0057] Accurately weigh 1.00g of polyε-caprolactone, add it into 5mL of chloroform, and prepare a 20w / v% solution, stir and dissolve evenly; accurately weigh 2.00g of polyethylene glycol, add 50ng of BMP-2 into 5mL of distilled water, and prepare into a 40w / v% solution, magnetically stirred to dissolve evenly. Inject into 5mL dry syringes respectively, the syringes are fixed in the card slot of the micro-injection pump, and the output end of the high-voltage DC power supply is fixed on the coaxial nozzle (the inner diameter of the outer needle is 1.1mm, the outer diameter of the inner needle is 0.6mm, and the inner diameter of the inner needle is 0.3 mm), the outer axis is polyε-caprolactone solution, and the flow rate is set to 0.5mL / h; the inner axis is polyethylene glycol solution, the flow rate is set to 0.1mL / h, and the DC high voltage is 20kV, which is rotated at a high speed. The roller is used as a receiver (rotating speed is 1200r / min), and the distance between the co...

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Abstract

The invention discloses a tissue engineering osteochondral composite support and a preparation method thereof, belonging to the technical field of biomaterials. The support is a multi-layer integrated structure, which is composed of a cartilage tissue support layer, a cartilage tissue calcification layer, a porous cell separation membrane and a bone tissue support layer. The cartilage tissue scaffold layer is inoculated with chondrocytes and introduced growth factors that promote chondrocyte formation to promote the growth of chondrocytes; the main raw materials such as heparan sulfate proteoglycan with good biocompatibility and degradability are selected, and cross-linking reaction, freeze-drying, and directional Pore-forming, multi-layer composite, complete integration and other methods to obtain functional multi-layer integrated tissue engineering osteochondral composite scaffold with good mechanical properties. The invention adopts micro-nano hydroxyapatite crystals and degradable non-stoichiometric polyethylene glycol / polyε-caprolactone nano-coaxial short fibers and heparan sulfate proteoglycan / oxidized sodium alginate grafted with RGD Compounded with N-succinyl chitosan, it is mainly used for repairing full-thickness defects of articular cartilage and subchondral bone.

Description

technical field [0001] The invention belongs to the technical field of biomaterial engineering, and relates to the preparation technology of tissue engineering scaffold of human articular cartilage. Background technique [0002] At present, cartilage defects at the end of movable joints caused by trauma, infection, tumor, and degeneration are common orthopedic clinical diseases. Cartilage wear and damage to articular surfaces. Because articular cartilage has no blood vessels, nerves, and lymphatic systems, cartilage defects with a diameter of more than 2-4 mm can hardly completely repair themselves; and clinically, simple articular cartilage injuries are relatively rare, and more are accompanied by subchondral bone lesions, including those caused by trauma Defect of subchondral bone, necrosis of subchondral bone caused by osteochondritis, sclerosis and fatty change of subchondral bone caused by cartilage degeneration. Therefore, it is necessary to repair the subchondral bo...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/46A61L27/54A61L27/38
CPCA61L27/3817A61L27/3821A61L27/3847A61L27/3852A61L27/46A61L27/54A61L2300/252A61L2300/414A61L2300/602A61L2430/02A61L2430/06A61L2430/24C08L5/10C08L5/04C08L5/08
Inventor 汪建新陈太军白家繁王莹莹翁杰冯波鲁雄周绍兵
Owner SOUTHWEST JIAOTONG UNIV
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