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ACE inhibition enzymatic hydrolysate and preparation method and application thereof

A technology of enzymatic hydrolysis solution and protease enzymatic hydrolysis solution, which is applied in the direction of pharmaceutical formula, hydrolyzed protein components, medical preparations containing active ingredients, etc., can solve the problems of low utilization rate, waste, waste disposal, etc., and achieve the promotion of commercial value and utilization rate, simple steps, and the effect of reducing resource waste and pollution

Inactive Publication Date: 2016-04-13
SHANGHAI JIAO TONG UNIV
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  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The scallop skirt is the mantle of the scallop. As the main by-product of the processing of the scallop stick, it accounts for 30.0% of the total weight of the scallop (except the shell). Its nutritional composition is similar to that of the scallop stick. nutritional value, but the current utilization rate is very low, except for a few that are treated as animal feed and sold at a low price, most of them are discarded as waste, resulting in a lot of waste and pollution

Method used

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  • ACE inhibition enzymatic hydrolysate and preparation method and application thereof
  • ACE inhibition enzymatic hydrolysate and preparation method and application thereof
  • ACE inhibition enzymatic hydrolysate and preparation method and application thereof

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Experimental program
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Effect test

Embodiment 1

[0032] Embodiment 1, the preparation method of ACE inhibition enzymolysis solution

[0033] 1. Pretreatment of scallop skirt:

[0034] Wash the purchased scallop skirt with water for 3 times. Then place it in an oven at 65°C overnight until dry. The dried scallop skirt is pulverized by a multifunctional pulverizer, and passed through a 60-mesh sieve to obtain scallop skirt powder, which is set aside.

[0035] 2. Analysis of nutritional components of scallop skirt:

[0036] By the Kjeldahl method, the protein content in the bay scallop skirt powder was measured to be 52.5±0.8%. The amino acid composition was analyzed by a fully automatic amino acid analyzer, and the result showed that the proline content was 207.53nmol / mg, accounting for 5.2% of the total protein content.

[0037] 3. Determination of degree of hydrolysis:

[0038] Improve according to the method of Adler-Nissen, the specific operation steps are as follows: take 0.1mL enzymolysis liquid sample in test tube,...

Embodiment 2

[0050] The IC of the ACE inhibitory enzymatic hydrolyzate obtained in embodiment two, embodiment one to ACE 50 Determination of

[0051] (1) Determination of ACE inhibition rate The reaction system is shown in Table 1. The experiment set up a blank group in which hydrochloric acid was added in advance to inactivate ACE, and a positive control group in which no ACE-inhibiting hydrolyzate was added.

[0052] Determination reaction system of table 1 ACE inhibition rate

[0053]

[0054] (2) The substrate hippuric acid histidine leucine (Hip-His-Leu, HHL) is catalyzed by ACE to generate hippuric acid. In the experimental group, the ACE inhibitory hydrolyzate can inhibit the activity of ACE and reduce the production of hippuric acid. In the blank group, hydrochloric acid inactivated ACE, theoretically unable to catalyze the formation of hippuric acid. In the positive control group, ACE inhibitory hydrolyzate was not added, ACE was not inhibited, and the production of hippuri...

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Abstract

The invention discloses a preparation method of an ACE inhibition enzymatic hydrolysate. The method comprises the following steps that 1, scallop skirts are washed to be clean, dried, smashed and screened, and scallop skirt powder is obtained; 2, the scallop skirt powder and alpha-chymotrypsin are added to a phosphate buffer solution, even mixing is conducted, a reaction is terminated at high temperature after hydrolysis is conducted, centrifugation is conducted, supernatant is taken, and an alpha-chymotrypsin hydrolysate is obtained; 3, the pH of the alpha-chymotrypsin hydrolysate is adjusted to 7.5 through a dipotassium phosphate solution, carboxypeptidase A and carboxypeptidase B are added, even mixing is conducted, a reaction is terminated at high temperature after hydrolysis is conducted, and a carboxypeptidase A / B hydrolysate is obtained; 4, dialysis is conducted on the carboxypeptidase A / B hydrolysate, a 500-1000 Da component is obtained, and the ACE inhibition enzymatic hydrolysate is obtained. The IC<50> value of the ACE inhibition enzymatic hydrolysate prepared through the preparation method to ACE is 893 micrograms / mL. The ACE inhibition enzymatic hydrolysate can be applied to preparation of depressurizing drugs and / or depressurizing foods.

Description

technical field [0001] The invention relates to an enzymolysis solution, in particular to an ACE-inhibiting enzymolysis solution and its preparation method and application. Background technique [0002] Hypertension is one of the most common and harmful diseases in the world. Studies have found that human blood pressure is mainly regulated by the renin-angiotensin system and the kallikrein-kinin system, and angiotensin-converting enzyme (ACE) has an important impact on both systems, which can lead to increased blood pressure. Therefore, inhibiting the activity of ACE can play a role in the treatment of hypertension. In addition to artificially synthesized puril drugs, food-derived ACE inhibitors have become a research hotspot because of their natural edible properties, safety and no side effects. ACE inhibitors can be prepared from various food materials through fermentation or enzymatic hydrolysis, and then separated and purified. [0003] The scallop skirt is the mantle...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06A61K38/01A61P9/12A23L33/18
CPCC12P21/06A61K38/012
Inventor 张建华吴秉宇姚晓敏钱炳俊汤慧勤
Owner SHANGHAI JIAO TONG UNIV
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