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Method for fermentation production of enramycin

A technology of enramycin and fermentation process, applied in the field of fermentation production of enramycin, can solve problems such as low production capacity and yield, achieve the effects of improving fermentation level, solving insufficient dissolved oxygen, and being simple and easy to operate

Active Publication Date: 2016-04-20
ANHUI BBCA FERMENTATION TECH ENG RES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

With the gradual elimination of other antibiotics, the use of enramycin is bound to become more and more widespread, but the industrial production of enramycin has been facing the problem of low production capacity

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] 1) Expansion of bacterial strains:

[0023] Take the frozen bacterial solution of bacterial strain FYFJ03 and dilute it into 10 6 spores / ml spore liquid, spread on flat solid medium, culture at 28°C for 7 days, select a single strain with regular shape and good growth, inoculate on solid slant medium, and cultivate at 28°C for 8 days.

[0024] Wherein, the solid medium includes the following components: 10 g / L of maltose, 1 g / L of dry yeast powder, 1 g / L of tuna extract, 1 g / L of tryptone, and 18 g / L of agar.

[0025] 2) Fermentation culture:

[0026] Inoculate the strains cultivated in step 1) into the fermentation medium of a 50 L fermenter that was sterilized beforehand under aseptic conditions, and culture at 28° C., with a fermentation period of 8 days. The concentration of magnesium sulfate is 0.05g / L, the concentration of ammonium chloride is 0.1g / L mixed solution and the volume of the mixed solution is 1.5% of the volume of the fermentation broth at 52 hours o...

Embodiment 2

[0029] The frozen bacterial solution of bacterial strain FYFJ03 was diluted to 10 6 spores / ml spore liquid, spread on flat solid medium, culture at 28°C for 7 days, select a single strain with regular shape and good growth, inoculate on solid slant medium, and cultivate at 28°C for 7 days.

[0030] Wherein, the solid medium includes the following components: corn flour 20g / L, peptone 1g / L, zinc sulfate 0.1g / L, manganese chloride 0.02g / L, agar 15g / L.

[0031] 2) Fermentation culture:

[0032] Inoculate the strains cultivated in step 1) into the fermentation medium of a 50 L fermenter that was sterilized beforehand under aseptic conditions, and culture at 28° C., with a fermentation period of 8 days. The concentration of magnesium sulfate is 0.08g / L, the concentration of ammonium chloride is 0.2g / L, and the volume of the mixed solution is 2% of the volume of the fermentation broth; the batch addition is started after 64 hours of fermentation The concentration of magnesium sulf...

Embodiment 3

[0035] Take the frozen bacterial solution of bacterial strain FYFJ03 and dilute it into 10 5 spores / ml spore liquid, spread on flat solid medium, culture at 28°C for 7 days, select a single strain with regular shape and good growth, inoculate on solid slant medium, and cultivate at 28°C for 7 days.

[0036] Wherein, the solid medium includes the following components: corn flour 20g / L, peptone 1g / L, zinc sulfate 0.1g / L, manganese chloride 0.02g / L, agar 15g / L.

[0037] 2) Fermentation culture:

[0038]Inoculate the strains cultivated in step 1) into the fermentation medium of a 50 L fermenter that was sterilized beforehand under aseptic conditions, and culture at 28° C., with a fermentation period of 8 days. The concentration of magnesium sulfate is 0.1g / L, the concentration of ammonium chloride is 0.3g / L mixed solution and the volume of the mixed solution is 3% of the fermentation broth volume; the batch addition is started after 64 hours of fermentation The concentration of ...

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Abstract

The invention relates to a method for fermentation production of enramycin. During the fermentation process of constant temperature culture in a fermentation culture medium, an inorganic salt solution is added in batches at different stages, synthesis of enramycin is promoted, oxygen dissolution of fermentation liquor can be improved, and the fermentation level of enramycin can be increased. The method is simple and feasible, and after fermentation of the process in the fermentation culture process, the fermentation level of enramycin can be increased by 25%.

Description

technical field [0001] The invention relates to the technical field of fermentation, in particular to a method for fermenting and producing enramycin. Background technique [0002] Enramycin (enramycin), also known as anlemycin or and duramycin, is a polypeptide animal antibiotic produced by the fermentation of fungicide Streptomyces. Enramycin is an organic base composed of 17 amino acid molecules and fatty acid molecules of 13 different types. The main components are enramycin A and B, which are applied in the form of hydrochloride. Enramycin has high stability in the feed, will not be degraded in the intestinal tract, and has a strong effect on Gram-positive bacteria. Long-term use will not produce drug resistance, and it can change the bacterial community in the intestinal tract The distribution is beneficial to the nutrient absorption of feed ingredients, which can promote the weight gain of animals and improve the utilization rate of feed. Enramycin was developed by...

Claims

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Application Information

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IPC IPC(8): C12P21/02
CPCC12P21/02
Inventor 王芳杨金环穆晓玲李维理韩小芬
Owner ANHUI BBCA FERMENTATION TECH ENG RES
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