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Method for preparing semen raphani separation protein powder and hydrolysis concentrated solution

A technology for separating proteins and raisins, applied in the field of vegetable protein, can solve the problems of difficulty in separating ethanol-water azeotrope, high consumption of high-concentration ethanol, and high production cost, and achieves simple and easy recovery process, low production cost, and easy operation. easy effect

Inactive Publication Date: 2016-05-11
CHONGQING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The main disadvantage of this method is: the use of about 70% ethanol leads to protein denaturation; high-concentration ethanol consumption is large, waste ethanol recovery needs distillation, rectification energy consumption is high, equipment investment is large, and ethanol-water azeotrope is difficult to separate, so , high production cost and uneconomical

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] 1. Preparation of radish seed residue

[0028] Be that the volume (L) ratio of 3 citric acid solutions is the ratio of 1: 4 according to defatted radish seed powder quality (kg) and pH, this citric acid solution is pumped into stainless steel hydrolysis tank earlier, open agitator and circulating hot water, wait When the citric acid solution was heated to 30°C. Add defatted radish seed powder, and then stir and hydrolyze at 30° C. for 4 hours. After the hydrolysis is completed, the hydrolyzate is discharged into the disc centrifuge, and the first centrifugation is carried out under the condition of 5000r / min, and the first hydrolysis centrifugation residue and the supernatant of the hydrolysis centrifugation are collected respectively. For the first hydrolysis and centrifugation slag collected, pump it into the washing kettle with a screw pump, add pure water to stir and wash twice, each washing time is 15 minutes, and the volume of pure water added during each washing...

Embodiment 2

[0040] 1. Preparation of radish seed residue

[0041] With embodiment 1, feature is: prepare the citric acid solution that pH is 3.5, be the ratio of 1: 6 according to the volume (L) ratio of defatted radish seed powder quality (kg) and citric acid solution, treat that citric acid solution is warming up to At 35°C, stirred and hydrolyzed at 35°C for 6 hours, centrifuged at 5500r / min, each washing time was 20 minutes, and centrifuged at 5500r / min.

[0042] 2. Preparation of Radix Seed Isolate Protein Extract

[0043] With embodiment 1, feature is: prepare the citric acid solution that pH is 6, the raspberry residue quality (kg) that collects by the 1st step and the ratio of citric acid solution volume (L) ratio are 1: 6, treat citric acid When the temperature of the solution was raised to 55°C, it was stirred and extracted at 55°C for 4 hours. Carry out centrifugal separation under the condition of 5500r / min, the addition volume of citric acid solution, pH value, concentratio...

Embodiment 3

[0053] 1. Preparation of radish seed residue

[0054] With embodiment 1, feature is: prepare the citric acid solution that pH is 4, be warming up to 40 ℃ according to the ratio of the volume (L) ratio of defatted radish seed powder quality (kg) and the volume (L) of citric acid solution of 1: 8, in Stir and hydrolyze at 40°C for 8 hours, then centrifuge at 6000r / min, wash for 30 minutes each time, and centrifuge at 6000r / min.

[0055] 2. Preparation of Radix Seed Isolate Protein Extract

[0056] With embodiment 1, feature is: prepare the citric acid solution that pH is 6.5, the raspberry residue mass (kg) that collects by the 1st step and the ratio of citric acid solution volume (L) ratio are 1: 8, treat citric acid When the temperature of the solution was raised to 65°C, it was stirred and extracted at 65°C for 5 hours. Carry out centrifugation under the condition of 6000r / min, the addition volume of citric acid solution, pH value, concentration, extraction time and the con...

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Abstract

The invention provides a method for preparing semen raphani separation protein powder and a hydrolysis concentrated solution and relates to methods and technologies of protein separation and hydrolysis. According to the method, degreased semen raphani powder is adopted as the raw material, a semen raphani separation protein extracting solution is prepared in the first place, and then semen raphani separation protein is prepared through separation and concentration by means of the ultrafiltration technology; finally, the semen raphani separation protein freeze-dried powder is prepared through freeze-drying; another process route is that proteolysis is conducted on the semen raphani separation protein; finally, the semen raphani separation protein concentrated solution is prepared through the reverse osmosis technology. The method has the advantages of being easy and convenient to operate, simple in equipment, mild in reaction condition, capable of saving energy, low in production cost and convenient to apply and popularize, and the method is an environment-friendly production process. A product prepared through the method can be widely applied to such fields as food and daily use chemicals.

Description

1. Technical field [0001] The invention belongs to the technical field of plant protein, and in particular relates to a preparation method of radish seed isolated protein powder and its hydrolysis application. 2. Background technology [0002] Isolate protein is a nitrogen-containing nutrient extracted and separated from animal, plant and microbial raw materials, and is widely used in food, medicine, daily chemical and chemical industries. For example, it is used in the production of edible protein powder, culture medium for antibiotics, nutraceuticals and protein surfactants. Today, my country's per capita protein intake is insufficient. The National Health and Family Planning Commission's "Chinese Resident Nutrition and Chronic Disease Status Report (2015)" pointed out that in 2012, the average protein intake of residents was 65 grams per person per day per person; while "China Food and Nutrition Development Outline (2014-2020)" shows that by 2020, the per capita protein i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K1/34C12P21/06A61K8/97A61K8/64A61Q19/00
CPCA61K8/645A61Q19/00C07K1/145C07K1/34C12P21/06Y02P20/10
Inventor 晏艳周小华石业新宿长琼余红梅杨洋袁德宽姚萍
Owner CHONGQING UNIV
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