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Eupolyphaga seu steleophaga small-peptide chelate capable of facilitating fibrinolysis and preparation method thereof

A technology of small peptide chelates and wood turtles, which is applied to the preparation method of peptides, skin care preparations, chemical instruments and methods, etc., can solve problems such as vision and dark adaptation effects, vitamin A can not normally play physiological functions, etc., to achieve Good economic benefits, low cost, and easy preparation

Pending Publication Date: 2016-05-11
BINZHOU MEDICAL COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

When zinc is deficient, vitamin A cannot normally play a physiological role, which affects vision and dark adaptation

Method used

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  • Eupolyphaga seu steleophaga small-peptide chelate capable of facilitating fibrinolysis and preparation method thereof
  • Eupolyphaga seu steleophaga small-peptide chelate capable of facilitating fibrinolysis and preparation method thereof
  • Eupolyphaga seu steleophaga small-peptide chelate capable of facilitating fibrinolysis and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0075] Crush the wood turtle through an 80-mesh sieve, add 12 times the amount of water to boil, wait until cooled to about 40°C, adjust the pH value to 2.0, add 1% pepsin, keep warm for 1 hour, adjust the pH value to 8.0, add 1% trypsin, and keep warm for 3 hour, boiled for 15 minutes, centrifuged, the supernatant was ultrafiltered through an ultrafiltration membrane with a relative molecular mass of 3KD, and the permeate was added to a DA201-C macroporous adsorption resin column, eluted with water, and the water wash was discarded, and then Elute with 90% ethanol, collect the ethanol eluate, recover ethanol under reduced pressure and continue to concentrate to a clear paste with a relative density of 1.10-1.15 at 60°C, spray dry (inlet air temperature is 180-200°C, outlet air temperature is 90 ~100°C) to obtain the small-peptide extract of the wood turtle.

[0076] Dissolve copper gluconate in distilled water to make a salt solution with a concentration of 15 mg / ml, adjust t...

Embodiment 2

[0078] Crush the wood turtle through an 80-mesh sieve, add 10 times the amount of water to boil, wait until it is cooled to about 45°C, adjust the pH value to 1.5, add 2% pepsin, keep warm for 2 hours, adjust the pH value to 9.0, add 2% trypsin, and keep warm for 4 hour, boiled for 15 minutes, centrifuged, the supernatant was ultrafiltered through an ultrafiltration membrane with a relative molecular mass of 3KD, and the permeate was added to a DA201-C macroporous adsorption resin column, eluted with water, and the water wash was discarded, and then Elute with 75% ethanol, collect the ethanol eluate, recover ethanol under reduced pressure and continue to concentrate to a clear paste with a relative density of 1.10-1.15 at 60°C, spray dry (inlet air temperature is 180-200°C, outlet air temperature is 90 ~100°C) to obtain the small-peptide extract of the wood turtle.

[0079]Dissolve zinc gluconate in distilled water to make a salt solution with a concentration of 10 mg / ml, adju...

Embodiment 3

[0081] Get the wood turtle small peptide chelate 100g that embodiment 1 method gains, by wood turtle small peptide chelate: corn oil: beeswax: lecithin ratio is 10:36:3:1 mixed material, grinds 30 minutes through colloid mill, with Gelatin, glycerin, purified water, and iron oxide black are used to make soft capsule skin glue at a ratio of 100:40:100:1. The temperature of the plastic box is controlled at 55±5°C, the temperature of the heat preservation tank is 40±5°C, and the temperature of the spray body is 36±5°C. , press soft capsules with a pill press, the content of each capsule weighs 0.50g, dry in cold air at 18°C ​​to 26°C for 4 hours (relative humidity is 30% to 40%) to shape, wash, dry, select pills, pack, and prepare into soft capsules. Capsule finished product.

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Abstract

The invention discloses a eupolyphaga seu steleophaga small-peptide chelate capable of facilitating fibrinolysis and a preparation method thereof, and belongs to the biotechnology field. The preparation method is characterized in that eupolyphaga seu steleophaga serves as the starting material, enzymolysis, ultrafiltration, resin column purification, chelating reaction and drying are conducted, the eupolyphaga seu steleophaga small-peptide chelate is obtained, and a corresponding preparation can be prepared by adding preparation auxiliaries through a conventional method. The eupolyphaga seu steleophaga small-peptide chelate has higher fibrinolysis facilitating activity and has the advantages of being free of toxic and side effects, free of pesticide damage, low in cost and the like.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a chelate of a drug for promoting fibrinolysis and a preparation method thereof. Background technique [0002] The ground beetle is the dried female body of Eupolyphagasinensis Walker or Steleophagaplancyi (Boleny), an insect of the cockroach family, which is a traditional Chinese medicine. Li Shizhen's "Compendium of Materia Medica" and other medical works of all dynasties have recorded that it is cold in nature, salty in taste, and slightly toxic. Pharmacological studies have shown that ground beetle has anti-ischemic hypoxia, immune enhancement, anti-hepatic fibrosis, anti-tumor, anti-thrombosis and other effects. As a traditional Chinese medicine, ground beetle is widely used in clinical practice, and its efficacy is reliable. In the clinical practice of traditional Chinese medicine, it can be administered orally with a single herb or a compatible compound to treat cardiovascula...

Claims

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Application Information

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IPC IPC(8): C07K7/06C12P21/06C07K1/36C07K1/34C07K1/16A61K38/01A61P7/02A61K8/98A61K8/64A61Q19/00
CPCC07K7/06A61K8/64A61K8/987A61K38/00A61K2800/10A61Q19/00C12P21/06
Inventor 代龙刘国飞柏道鸣
Owner BINZHOU MEDICAL COLLEGE
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