Pigskin small peptide chelate and preparation method and application thereof

A technology of small peptide chelate and pig skin, which is applied in the preparation method of peptide, preparation for skin care, chemical instrument and method, etc., achieves the effects of low cost, less toxic and side effects, and easy preparation

Inactive Publication Date: 2016-05-11
山东禹泽药康产业技术研究院有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Pigskin is a common food, but as a medicine, it has been recorded for a long time

Method used

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  • Pigskin small peptide chelate and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0067] Cut fresh pigskin into pieces, add 10 times the amount of 2% sodium carbonate solution to soak for 6 hours, discard the lye, rinse with water until it is nearly neutral, add 10 times the amount of water to homogenate, heat to boil, and cool to about 40°C. Adjust the pH value to 2.0, add 1% pepsin, keep warm for 1 hour, adjust the pH value to 8.0, add 1% trypsin, keep warm for 3 hours, boil for 15 minutes, centrifuge, and the supernatant is passed through an ultrafiltration membrane with a relative molecular mass of 3KD. Filtrate, add the permeate to the DA201-C macroporous adsorption resin column, elute with water, discard the water wash, and then elute with 90% ethanol, collect the ethanol eluate, recover ethanol under reduced pressure and continue to concentrate to 60% The clear ointment with a relative density of 1.10-1.15 °C is spray-dried (inlet air temperature is 180-200 °C, outlet air temperature is 90-100 °C) to obtain pigskin small peptide extract.

[0068] Dis...

Embodiment 2

[0070] Cut fresh pigskin into pieces, add 12 times the amount of 3% sodium carbonate solution to soak for 6 hours, discard the lye, rinse with water until it is nearly neutral, add 12 times the amount of water to homogenate, heat to boil, and cool to about 45°C. Adjust the pH value to 1.5, add 2% pepsin, keep warm for 2 hours, adjust the pH value to 9.0, add 2% trypsin, keep warm for 4 hours, boil for 15 minutes, centrifuge, and the supernatant is passed through an ultrafiltration membrane with a relative molecular mass of 3KD. Filtrate, add the permeate to the DA201-C macroporous adsorption resin column, elute with water, discard the water wash, and then elute with 75% ethanol, collect the ethanol eluate, recover ethanol under reduced pressure and continue to concentrate to 60% The clear ointment with a relative density of 1.10-1.15 °C is spray-dried (inlet air temperature is 180-200 °C, outlet air temperature is 90-100 °C) to obtain pigskin small peptide extract.

[0071] Di...

Embodiment 3

[0073]Get the pigskin small peptide chelate 100g that embodiment 1 method gained, by pigskin small peptide chelate: corn oil: beeswax: lecithin ratio is 10:36:3:1 mixed material, grinds 30 minutes through colloid mill , use gelatin, glycerin, purified water, iron oxide black to make soft capsule skin glue in a ratio of 100:40:100:1, control the temperature of the plastic box at 55±5°C, the temperature of the heat preservation tank at 40±5°C, and the temperature of the spray body at 36±5°C 5°C, press the soft capsules with a pellet machine, the content of each capsule weighs 0.50g, dry in cold air at 18°C-26°C for 4 hours (relative humidity is 30%-40%) to shape, wash the pills, dry, select the pills, pack, and prepare Become soft capsule finished product.

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Abstract

The invention discloses a pigskin small peptide chelate and a preparation method and application thereof and belongs to the field of biotechnology. The pigskin small peptide chelate and the preparation method are characterized in that pigskin serves as a starting material, enzymolysis, ultrafiltration, resin column purification, a chelating reaction and drying are conducted, the pigskin small peptide chelate is obtained, and preparation auxiliaries can be added, and the pigskin small peptide chelate can be prepared into corresponding preparations according to a conventional method. By the adoption of the pigskin small peptide chelate, wound healing can be remarkably promoted, the beautifying and anti-wrinkle functions are achieved, and the pigskin small peptide chelate has the advantages of being free of toxic or side effect, free of drug injury and low in cost.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a small pigskin peptide chelate and its preparation method and application. Background technique [0002] Pigskin was first recorded in "Decoction of Materia Medica". Since ancient times, pigskin has been used in the treatment of various related diseases, and it is mostly used in the treatment of surgical skin injuries in modern clinical practice. The protein content in pigskin is as high as 33%, of which the collagen content is 87.8%. Pig skin is similar to human skin in structure, rich in type I and type III collagen, and has better homology with humans than cows and rats, and has fewer potential sources of disease. In addition, my country is a large pig-producing country with abundant high-quality pig skin. Inexpensive pigskin resources, people are more and more in-depth research on extracting collagen from pigskin, and now there are more and more researches on the preparation of ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06C07K14/78C07K1/36C07K1/34C07K1/16C07K1/107A61K8/65A61K8/98A61K35/36A61K38/39A61P17/02A61Q19/08
CPCC12P21/06A61K8/65A61K8/985A61K9/4858A61K9/4866A61K35/36A61K38/39A61Q19/08C07K14/78
Inventor 代龙柏道鸣卢宁
Owner 山东禹泽药康产业技术研究院有限公司
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