Composite soil modifying agent and preparation method and application thereof
A technology of soil conditioner and microbial inoculum, which is applied in the field of composite soil conditioner and its preparation, can solve the problems of inability to stably meet the needs of nutrition, high requirements on microbial environment, unstable microbial action, etc., so as to increase the number of viable bacteria and Survival Rate, Survival Rate Increase, Survival Rate, and Resurrection Rate Increased Effects
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Embodiment 1
[0039] 1. Preparation of microbial agents
[0040] Bacillus subtilis, Bacillus jelly-like, Bacillus megaterium and Bacillus thuringiensis are respectively activated, and after activation, they are respectively inoculated into respective strain culture media to obtain the strain culture liquid of each Bacillus.
[0041] After expanding the culture medium of each Bacillus strain, inoculate them into their respective main fermentation tanks, and cultivate them at a temperature of 35-37°C, a pH value of 7.0-7.5, and a stirring rate of about 150r / min , Cultivate about 8-10h to reach the logarithmic growth phase, then add about 0.1ppm of micro-nano silicon (particle size is 100 nanometers-10 microns) to the fermentation broth of each bacillus, continue to cultivate about 30min, and then add each spore About 0.1 ppm of nano-carbon (with a particle size of 5-150 nanometers) was added to the fermentation liquid of the bacillus, and the culture was continued for about 20 hours to obtain...
Embodiment 2
[0051] 1. Preparation of microbial agents
[0052] Bacillus subtilis, Bacillus jelly-like, Bacillus licheniformis and Bacillus thuringiensis are respectively activated, and after activation, they are respectively inoculated into respective strain culture medium to obtain the strain culture liquid of each Bacillus.
[0053] After expanding the culture medium of each Bacillus strain, they were inoculated into their respective main fermentation tanks, and fermented at a temperature of 32-35°C, a pH value of about 6.5, and a stirring rate of about 200r / min. Cultivate for about 8-10 hours to reach the logarithmic growth phase, then add about 0.01ppm of nano-silicon material (particle size is 100 nanometers to 10 microns) to the fermentation broth of each bacillus, continue to cultivate for about 60 minutes, and then add each bacillus About 0.01ppm of carbon nanomaterials (with a particle size of 5-150 nanometers) were added to the fermentation liquid of the above-mentioned fermente...
Embodiment 3
[0063] 1. Preparation of microbial agents
[0064] Bacillus subtilis, Bacillus jelly-like, Bacillus licheniformis and Bacillus thuringiensis are respectively activated, and after activation, they are respectively inoculated into respective strain culture medium to obtain the strain culture liquid of each Bacillus.
[0065] After expanding the culture medium of each bacillus, they were inoculated into their respective main fermenters, and cultivated under the conditions of a temperature of 33-37°C, a pH value of 7-7.5, and a stirring rate of 120r / min. Cultivate about 8-10h to reach the logarithmic growth phase, then add about 1ppm of nano-silicon material (particle size is 100 nanometers to 10 microns) to the fermentation broth of each bacillus, continue to cultivate for about 30min, and then add to the fermentation broth of each bacillus About 1ppm of micro-nano carbon (particle size: 5-150 nanometers) is added to the fermentation liquid, and the culture is continued for about...
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