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Application of streptomyces lavendulae to preventing and treating dry rot of actinidia arguta

A technology of Streptomyces lavender and Actinidia jujube, which is applied in the field of agricultural biology, can solve the problems of fruit pesticide residues and environmental pollution, and achieve the effect of environmental friendliness and good control effect

Inactive Publication Date: 2016-06-08
EASTERN LIAONING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to provide the application of a strain of Streptomyces lavender in the prevention and treatment of kiwi fruit dry rot, so as to solve the problems of environmental pollution and fruit pesticide residues caused by chemical pesticide control

Method used

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  • Application of streptomyces lavendulae to preventing and treating dry rot of actinidia arguta
  • Application of streptomyces lavendulae to preventing and treating dry rot of actinidia arguta
  • Application of streptomyces lavendulae to preventing and treating dry rot of actinidia arguta

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] Example 1 Isolation and screening of antagonistic bacteria

[0015] 1. Separation and purification

[0016] From June 2013 to August 2015, 50 soil samples were collected from Fengcheng, Donggang, Kuandian and other places in Liaoning Province using the five-point sampling method. The collected soil samples were air-dried naturally. Take 10g of soil sample, place it in a conical flask filled with 90ml of sterile water, and shake it on a shaker for 20min. After standing for 30s, dilute 10 times successively to prepare 10 -4 Diluent. Micropipette pipette 10 -4 Put 0.1ml of the diluent on the solid plate of Gao's No. 1 medium, and spread it evenly with a sterile applicator. 25 ℃ constant temperature incubator upside down for 3-5 days. The strains with different colony forms on the plate were picked and transferred to Gao’s No. 1 medium to obtain pure culture, sealed in sterilized paraffin oil, and stored at 4°C for later use. Gaoshi No. 1 medium: 20.0g soluble starch...

Embodiment 2

[0020] Identification of embodiment 2 antagonistic strains

[0021] 1. Morphological identification and biochemical characteristics

[0022] Colony morphology: the aerial hyphae of strain DTJ-24 on Gao’s No. 1 medium are lilac-colored, and the hyphae in the base are colorless and have no soluble pigments; microscopic morphology: the hyphae in the base have no septum and no Fracture; the aerial hyphae are multi-branched, and the branches are relatively short, the spore filaments are long and spiral, and the spores are elliptical to cylindrical, with smooth surface.

[0023] Biochemical characteristics: Strain DTJ-24 can liquefy gelatin and hydrolyze starch weakly. Produces hydrogen sulfide, can decompose cellulose, peptonizes milk, and does not coagulate. Glucose and fructose can be used, but sucrose, inositol, mannitol, raffinose, and D-xylose are not used.

[0024] 2. 16SrDNA sequence analysis

[0025] The strain DTJ-24 was inoculated in Gaoshi No. 1 liquid medium, cultur...

Embodiment 3

[0027] Embodiment 3 Preparation of antagonistic bacteria aseptic fermentation filtrate

[0028] The strain DTJ-24 was inoculated in the liquid medium, the medium loading was 70%, the volume of the fermenter was 30L, the stirring speed was 150-200rpm / min, the ventilation rate was 15-20%, the temperature was 25-30℃, and the pH value was 6.5~ Under the condition of 7.5, constant temperature fermentation culture was carried out. After 5 days of fermentation, the fermentation liquid was centrifuged at 5000r / min to prepare a supernatant, which was filtered with a sterile filter membrane to prepare a sterile fermentation filtrate.

[0029] The formula of the liquid medium is as follows: yeast extract powder 4.0g / L, malt extract powder 10.0g / L, glucose 4.0g / L, water 1000ml, pH value 7.5.

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Abstract

The invention discloses application of streptomyces lavendulae to preventing and treating dry rot of actinidia arguta and relates to the field of the agricultural biotechnology. The streptomyces lavendulae is preserved in China General Microbiological Culture Collection Center, and the preservation number is CGMCC No.9972. Sterile fermentation filtrate 100-fold diluent of the streptomyces lavendulae is directly sprayed to the stems of the actinidia arguta or poured to the roots of the actinidia arguta, and the prevention effect on dry rot of the actinidia arguta can reach 88.6%. The process is simple, cost is low, use is convenient, use of chemical pesticide in the cultivation process of the actinidia arguta can be reduced, the advantages of being safe and environmentally friendly are achieved, and good application and development prospects are achieved.

Description

technical field [0001] The invention relates to the application of a strain of Streptomyces lilacaceae in preventing and treating dry rot of Actinidia jujube, belonging to the field of agricultural biotechnology. Background technique [0002] Actinidia arguta (Sieb.etZucc.) Planch.ExMiq. Also known as soft jujube, kiwi pear, vine melon, vine pear, it belongs to Actinidiaceae, and kiwifruit is a large deciduous vine. The fruit of kiwi fruit is small, smooth, edible and nutritious. It is a new commercial fruit and has been introduced and cultivated in large areas by Canada, Chile, France, New Zealand and the United States. The commercial cultivation of kiwifruit in China has just started. At present, the cultivated area in Dandong, Liaoning has reached more than 10,000 mu, which is the largest cultivated area of ​​kiwifruit in China. [0003] In the past two years, a new disease, kiwifruit dry rot, has appeared in the plantations of kiwifruit in Dandong area. The disease ma...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N63/02A01P3/00
CPCA01N63/10
Inventor 李娟王旭王东来
Owner EASTERN LIAONING UNIV
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