Preparation method of intervertebral disc fibrous ring tissue engineering scaffold

A technology of tissue engineering scaffold and annulus fibrosus, which is applied in tissue regeneration, medical science, prosthesis, etc., can solve the problems of low simulation degree of fibrous annulus scaffold structure, lack of mechanical properties, and uncontrollable degradation rate, etc., to achieve beneficial Effects of Adhesive Growth, Good Biocompatibility, and High Porosity

Inactive Publication Date: 2016-06-15
KUNMING UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The technical problem to be solved by the present invention is that the simulation degree of the existing fiber ring support structure is not high, the degradation rate is uncontrollable, and the mechanical properties do not have anisotropy, etc.

Method used

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  • Preparation method of intervertebral disc fibrous ring tissue engineering scaffold
  • Preparation method of intervertebral disc fibrous ring tissue engineering scaffold
  • Preparation method of intervertebral disc fibrous ring tissue engineering scaffold

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] (1) Preparation of stock solution: add gelatin to distilled water and let it stand for 2 hours, then heat it in a water bath to 50°C, stir well until the gelatin is completely dissolved; then add nano-hydroxyapatite powder, and continue stirring until nano-hydroxyapatite powder is formed Form a uniform suspension state in the solution; add konjac glucomannan and stir until the konjac glucomannan is fully mixed with the suspension, the stirring speed is 1300r / min, and the time is 30min; After completion, take out and cool to room temperature to obtain stock solution; the concentration of konjac glucomannan in the stock solution is 30g / L, the concentration of gelatin is 25g / L, and the concentration of nano-hydroxyapatite is 15g / L.

[0032] (2) Film making: Pour the original solution on a specific template, the original solution flows under the action of gravity and spreads on the template to form a uniform thin layer, the thickness of the thin layer is 1mm, and then the fi...

Embodiment 2

[0040] (1) Preparation of stock solution: add gelatin to distilled water and let it stand for 5 hours, then heat it in a water bath to 60°C, stir well until the gelatin is completely dissolved; then add nano-hydroxyapatite powder, and continue stirring until nano-hydroxyapatite powder is formed Form a uniform suspension state in the solution; add konjac glucomannan and stir until the konjac glucomannan is fully mixed with the suspension, the stirring speed is 1400r / min, and the time is 40min; After completion, take out and cool to room temperature to obtain stock solution; the concentration of konjac glucomannan in the stock solution is 25g / L, the concentration of gelatin is 30g / L, and the concentration of nano-hydroxyapatite is 20g / L.

[0041] (2) Film making: Pour the original solution on a specific template, the original solution flows under the action of gravity and spreads on the template to form a uniform thin layer, the thickness of the thin layer is 3mm, and then the fi...

Embodiment 3

[0050] (1) Preparation of stock solution: Add gelatin to distilled water and let it stand for 12 hours, then heat it in a water bath to 70°C, stir well until the gelatin is completely dissolved; then add konjac glucomannan and stir until the konjac glucomannan is fully dissolved, the stirring speed is 1200r / min for 50min; then place it in a microwave oven with a power of 700W and heat it for 30s. After the heating is completed, take it out and cool it to room temperature to obtain the stock solution; the concentration of konjac glucomannan in the stock solution is 20g / L, and the concentration of gelatin is 35g / L.

[0051] (2) Membrane making: Pour the original solution on a specific template, and the original solution flows under the action of gravity and spreads on the template to form a uniform thin layer. Thin layer thickness is 2 mm. Subsequently, the film was dried in a drying oven at 55°C.

[0052] (3) Cross-linking of the membrane: The dried membrane was cross-linked b...

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Abstract

Belonging to the application field of biomedical material technologies, the invention discloses a preparation method of an intervertebral disc fibrous ring tissue engineering scaffold. The method provided by the invention adopts degradable biomaterial konjac glucomannan (KGM), gelatin (Gelatin) and nano-hydroxyapatite (Nano HAP) with good biocompatibility as the basic raw materials, utilizes film coiling technique to perform crosslinking with ammonia water or glutaraldehyde as the crosslinking agent, and then conduct freeze drying to obtain an anisotropic intervertebral disc fibrous ring tissue engineering scaffold. According to the preparation method of the scaffold, the product has the advantages of high simulation structure, anisotropy, high porosity, arbitrarily designable shape and size, and adjustable degradation rate, etc. As an intervertebral disc fibrous ring repair material, the intervertebral disc fibrous ring tissue engineering scaffold prepared by the method can have good application prospect.

Description

technical field [0001] The invention relates to a preparation method of an intervertebral disc annulus fibrosus tissue engineering support, which belongs to the technical application field of biomedical materials. Background technique [0002] Degenerative disc disease is a common disease in today's society. In the United States, it ranks second only to upper respiratory tract infection, and is one of the main causes of labor loss; Asian countries such as China have a higher incidence because there are more manual workers. With the development of my country's aging process, intervertebral disc degenerative disease has increasingly become a disease that seriously affects people's work and life. The emergence of tissue engineering technology provides new ideas and methods for the treatment of intervertebral disc diseases, and is expected to provide permanent replacement products for intervertebral disc repair or replacement. Among them, the regeneration and repair of the fib...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/24A61L27/20A61L27/12A61L27/56
CPCA61L27/24A61L27/12A61L27/20A61L27/56A61L2300/112A61L2300/232A61L2300/252A61L2430/38C08L5/02
Inventor 陈希亮陈庆华颜廷亭
Owner KUNMING UNIV OF SCI & TECH
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