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Endotoxin adsorption material for blood purification and preparation method and application of endotoxin adsorption material for blood purification

A technology for blood purification and adsorption materials, applied in chemical instruments and methods, blood circulation treatment, suction devices, etc., can solve the problems of large batch differences, many reaction steps, and unstable performance of adsorption material products, and improve the binding efficiency. , The effect of simplifying the preparation process and reducing the difference between batches

Inactive Publication Date: 2016-06-15
GUANGZHOU KONCEN BIOSCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Although these two methods avoid the use of the highly toxic substance cyanogen bromide, and the materials prepared by them are safer to use, but there are many reaction steps in the preparation process, and five steps of chemical reactions are required to synthesize the adsorption material, and the method is complicated, so the obtained Batch-to-batch variation of adsorption material products is large, and the performance is unstable

Method used

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  • Endotoxin adsorption material for blood purification and preparation method and application of endotoxin adsorption material for blood purification
  • Endotoxin adsorption material for blood purification and preparation method and application of endotoxin adsorption material for blood purification

Examples

Experimental program
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Effect test

Embodiment 1

[0041] 1. Reaction of agarose gel with ethylene glycol bisglycidyl ether

[0042] (1) Carrier activation: Add 0.5 L of agarose gel (Cl-6B, particle size 300-600 μm) and 1.0 mol / L NaOH aqueous solution (containing 5 mg / mL of NaBH) into a 2.0 L reactor 4 solution) 20mL, mixed evenly, added 500mL of ethylene glycol bisglycidyl ether, placed in a constant temperature shaker, reacted at 40°C for 5h, and ended the reaction to obtain an activated carrier;

[0043] (2) Filter the activated carrier obtained in step (1), and use 20 times the volume of distilled water, ethanol / water (1:1 by volume ratio), absolute ethanol, ethanol / water (1:1 by volume ratio) and Rinse with distilled water;

[0044] Store the activated carrier at 4°C for later use; use the sodium thiosulfate method to detect the number of epoxy groups in the gel activated by this method, and measure at least 50 μmol of epoxy active groups per milliliter, which is recorded as E1-1.

[0045] 2. Reaction of agarose gel wi...

Embodiment 2

[0062] Synthesis of endotoxin-absorbing materials

[0063] 1. Reaction of active agarose gel (Cl-6B) with polylysine and glycine to synthesize endotoxin adsorption material for blood purification

[0064] Take 10 g of active agarose gel (E1-1) and add 20 mL of an aqueous solution containing 1.0 g of polylysine and 0.2 g of glycine, adjust the pH to 12, and react at 60°C for 24 hours; wash with distilled water after the reaction; then add 20mL of 1.0mol / L ethanolamine solution, adjust the pH to 8.0, capping reaction, shaking reaction at 37°C for 4 hours; after the reaction is completed, wash with distilled water to obtain endotoxin adsorption material E1-1-1 for blood purification.

[0065] 2. Reaction of active agarose gel (Cl-6B) and arginine to synthesize endotoxin adsorption material for blood purification

[0066] Take 10g of active agarose gel (E1-1) and add 60mL of aqueous solution containing 0.8g of arginine, adjust the pH value to 5.0, and react at 37°C for 24 hours; ...

Embodiment 3

[0098] Adsorption endotoxin test

[0099] The endotoxin concentration of patients with endotoxemia is generally less than 1EU / mL, and the initial concentration of adsorption is set to be 1EU / mL in the experiment. Take a 10 mL pyrogen-free Erlenmeyer flask, add 0.2 g of the endotoxin adsorption material for blood purification prepared in Example 2, and then add 6 mL of plasma containing endotoxin 1 EU / mL, and oscillate for 2 hours (temperature 37 ° C, oscillating Speed ​​180rpm), then use the dynamic turbidity method to measure the endotoxin concentration after adsorption, and calculate its adsorption capacity and clearance rate by the adsorbent, the results are shown in Table 1.

[0100] Adsorption capacity and clearance rate of endotoxin by each endotoxin adsorption material for blood purification in Example 2 in Table 1

[0101]

[0102]

[0103]It can be seen from the test results that the endotoxin adsorption materials for blood purification in the examples of the p...

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Abstract

The invention discloses an endotoxin adsorption material for blood purification and a preparation method and application of the endotoxin adsorption material for blood purification.The endotoxin adsorption material for blood purification is obtained by coupling reaction of an active carrier and genins containing aminos, wherein the ratio of the active carrier to every genin is 10:1-100:1; the active carrier is obtained by reaction between a carrier and a bis-glycidyl ether coupling reagent, wherein the carrier is a spherical natural polymer with the particle size being 0.05-1.5mm, and the volume ratio of the carrier to the bis-glycidyl ether coupling reagent is 1:0.5-1:10.The endotoxin adsorption material for blood purification has the advantages of high specificity, good blood compatibility, high endotoxin adsorption efficiency and capability of being used for blood purification and adsorption treatment of endoxemia clinically.The preparation method is simple, convenient, simple and short in process route and safe for preparation.

Description

technical field [0001] The invention relates to the field of medical biological materials, in particular to an endotoxin adsorption material for blood purification and its preparation method and application. Background technique [0002] Endotoxin is the main component of the outer wall of the cell membrane of gram-negative bacteria (GNB), and plays an important role in the stability of bacteria. Endotoxin is almost ubiquitous, is a highly toxic inflammatory and pyrogenic substance, and is the main pathogenic medium of endotoxemia and septic shock. Endotoxemia is a condition caused by the release of a large amount of bacterial endotoxin into the blood by bacteria in the blood or in the lesion, or the transfusion of a large amount of endotoxin-contaminated fluid. When the body is subjected to trauma (including surgery), burns, infection, radiotherapy, chemotherapy, and long-term traditional parenteral nutrition, the permeability of the intestinal mucosa increases, and the pe...

Claims

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Application Information

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IPC IPC(8): B01J20/24B01J20/32A61M1/36
CPCB01J20/24A61M1/3612A61M2202/0415A61M2205/02A61M2207/10B01J20/22B01J20/26B01J20/3217
Inventor 陈校园李永桂杨正根曾萍胡家亮
Owner GUANGZHOU KONCEN BIOSCI
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