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Method and application for screening bacteria capable of relieving constructed wetland blocking

A technology of constructed wetlands and screening methods, applied in the direction of bacteria, chemical instruments and methods, and special treatment targets, etc., can solve the problems of destructive physical and chemical measures in constructed wetlands, and achieve low toxicity, ease of blockage, and low energy consumption Effect

Inactive Publication Date: 2016-06-15
INST OF AQUATIC LIFE ACAD SINICA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] In order to solve the problem of large destructive physical and chemical measures in constructed wetlands, the purpose of the present invention is to provide a screening method for bacteria that relieve congestion in constructed wetlands. The screening process of the method is simple and easy to apply

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] A screening method for constructing wetland blockage-relieving bacteria, the steps of which are:

[0039] A. Bacterial enrichment: Collect blockages in constructed wetlands that have been built and operated, take 2g of blockages, add 100ml of sterile water, shake on a shaker for 3 or 4 or 5 hours, take 10ml of supernatant and inoculate into 90ml enrichment In culture medium, culture at 28 or 29 or 30 or 31 or 32°C, 120r / min for 2 or 3 or 4 days. Then take the culture solution and transfer it to the enrichment medium, and repeat the culture for 1, 2 or 3 times to obtain the culture solution.

[0040] The enrichment medium consists of: ammonium nitrate hexahydrate (chemical formula: NH 4 NO 3 ·6H 2 O) 1g, potassium dihydrogen phosphate (chemical formula: KH 2 PO 4 ) 1g, dipotassium hydrogen phosphate (K 2 HPO 4 ) 1g, magnesium sulfate heptahydrate (chemical formula: MgSO 4 ·7H 2 O) 0.2g, calcium chloride dihydrate (chemical formula: CaCl 2 2H 2 O) 0.02g, ferrou...

Embodiment 2

[0051] A kind of artificial wetland clogging relieving bacteria application in alleviating constructed wetland clogging, its steps are:

[0052] (1) According to Example 1, bacteria capable of producing rhamnolipids were screened out.

[0053] (2) Carry out enrichment culture of the screened bacterial species, and take the supernatant to measure the concentration of surfactant in the culture solution.

[0054] (3) Dosing the culture solution into the wetland influent, so that the dosage of the surfactant is 0.09 or 0.1 or 0.13 or 0.15g / L.

[0055] (4) The water enters the wetland and stays for 12 or 14 or 16 or 18 or 20 hours to dissolve and block the wetland. The rhamnolipid has a hydrophilic group and a hydrophobic group, which can reduce the interface and surface tension of the blockage. The hydrophobic base end combines with the organic group of the extracellular polymer, and the hydrophilic end combines with the pore water of the blockage in the constructed wetland, chan...

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Abstract

The invention discloses a method and application for screening bacteria capable of relieving constructed wetland blocking.The method comprises the steps of 1 bacteria enrichment, wherein wetland blockages are taken for bacteria enrichment; 2, bacteria preliminary screening, wherein enriched bacteria are cultured through a thickened filter paper culture medium, the bacteria producing cellulase degeneration enzymes can form spots on filter paper, and the spots are taken for pure culture; 3, bacteria secondary screening, wherein a bacterial strain subjected to the pure culture is inoculated into a blood agar culture medium, and a bacterial colony producing blood-plate hemolysis is taken; 4, bacteria optimization, wherein surfactant can form oil spreading circles on oil film, and bacteria of the largest oil spreading circle serve as optimal bacteria; and 5, screening bacteria product identification.According to the application for screening the bacteria capable of relieving constructed wetland blocking, the strains producing biosurfactant with larger yield are screened out; the screened strains produce the biosurfactant to relieve constructed wetland blocking, screened bacteria sources adopt constructed wetland bacteria, and the produced biosurfactant is efficient, easy to biologically degrade and capable of safely relieving constructed wetland blocking.

Description

technical field [0001] The invention belongs to the field of biotechnology, and more specifically relates to a method for screening bacteria for relieving clogging in constructed wetlands, and also relates to an application of bacteria for relieving clogging in constructed wetlands, which is suitable for applying the culture solution of optimal bacteria screened to effective Clogging tendencies or already clogged constructed wetlands to relieve clogging. technical background [0002] Constructed wetlands are wetlands that are artificially built and controlled, and their mechanisms include microbial decomposition, plant absorption and transformation, matrix adsorption and filtration, and other functions. General constructed wetlands mainly use the physical, chemical and biological synergies of artificial substrates, plants and microorganisms to purify and treat sewage. Compared with traditional water treatment technology, constructed wetland has the following advantages: (1)...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F3/32C02F3/34
CPCC02F3/327C02F3/34C02F3/342C02F2303/14C12N1/20Y02W10/10
Inventor 吴振斌杜明普徐栋王媚武俊梅张义贺锋周巧红
Owner INST OF AQUATIC LIFE ACAD SINICA
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