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Grape-seed specific mean-degree-of-polymerization procyanidin extract and preparing method and application thereof

A technology of proanthocyanidins and grape seeds, applied in the field of medicine, can solve the problems of no patents or literature reports on grape seed-related proanthocyanidins extracts, few single traditional Chinese medicines or plant extracts or active ingredients, etc.

Inactive Publication Date: 2016-07-20
TIANJIN INSTITUTE OF PHARMA RESEARCH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0011] At present, there are few reports on the use of a single traditional Chinese medicine or plant extract or active ingredient with a clear material basis for the treatment of diarrhea, and there are no patents or literature reports on the use of grape seed-related proanthocyanidin extracts for the treatment of diarrhea

Method used

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  • Grape-seed specific mean-degree-of-polymerization procyanidin extract and preparing method and application thereof
  • Grape-seed specific mean-degree-of-polymerization procyanidin extract and preparing method and application thereof
  • Grape-seed specific mean-degree-of-polymerization procyanidin extract and preparing method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0079] Take 500 g of grape seed raw material, crush it through a 100-mesh sieve, stir and extract with 10 times 70% acetone-water, divide the extract into two parts, concentrate one part to a thick paste, and dry to obtain the total grape seed extract (45 g); The other part was evaporated to remove acetone, and water was added to a total volume of 2000ml; the above-mentioned concentrated solution was extracted with ethyl acetate, extracted 3 times with equal volume, and the organic layer was discarded; Insoluble matter 1, 3.2g was obtained; an equal volume of dichloromethane was added to the filtrate, and after mixing, it was filtered (insoluble matter 2, 9.7g, was set aside); the filtrate was evaporated to dryness, redissolved in methanol to a saturated solution, and added 1.5 times (v / v) of dichloromethane, after mixing, filter (insoluble matter 3, 3.4g, set aside); evaporate the filtrate to dryness, redissolve with methanol to a saturated solution, add 2.3 times (v / v) dichlo...

Embodiment 2

[0092] Take 50 g of grape seed crude extract (total proanthocyanidin content is 30%), redissolve with about 20 times of water, discard the insoluble matter, and obtain an aqueous solution of grape seed crude extract for later use; extract the above aqueous solution with ethyl acetate, and extract with equal volume 3 times, discard the organic layer; evaporate the extracted aqueous layer solution to dryness, redissolve with 2000ml methanol, filter (to obtain insoluble matter 1, 4.6g); add an equal volume of chloroform, mix well, and filter (to obtain insoluble matter 2, 7.5g); the filtrate was evaporated to dryness, redissolved with methanol to a saturated solution, added 1.5 times (v / v) of chloroform, mixed, filtered (to obtain insoluble matter 3, 3.7g); the filtrate was evaporated to dryness, and dissolved in methanol Redissolve into a saturated solution, add 2.3 times (v / v) of chloroform, mix and filter to obtain an insoluble matter, dry to obtain the target extract (6.8g); e...

Embodiment 3

[0101] Take 1000g of grape seed raw material, crush it to 80 mesh, percolate with 20 times of 70% ethanol water, concentrate the percolation liquid to 0.2g / mL (based on grape seed raw material); extract the above concentrated solution with ethyl acetate, and extract with equal volume 3 times, discard the organic layer; divide the aqueous layer solution into 2 parts, wherein 1 part is concentrated and dried to obtain grape seed total proanthocyanidins (90g); after the other part of the aqueous layer solution is concentrated, pass the D101-I weak polar Pore ​​resin purification, eluted with 20% ethanol for 2 column volumes after loading the sample, collected the eluate, concentrated, and dried to obtain 20% ethanol eluted fraction (54g); eluted with 30% ethanol for 4 column volumes, collected The 30% eluate was concentrated and dried to obtain the target extract (17.5 g).

[0102] Adopt n-butanol-hydrochloric acid method to measure the total proanthocyanidin content of grape see...

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Abstract

The invention relates to a grape-seed specific mean-degree-of-polymerization procyanidine extract and a preparing method and application thereof. According to the grape-seed specific mean-degree-of-polymerization procyanidine extract and the preparing method and application thereof, the specific mean-degree-of-polymerization procyanidin extract with the mean degree of polymerization (mDP) of 4.0-9.0 and the total content of anthocyanin larger than or equal to 50% is obtained from grape seeds through solvent fractional precipitation or resin gradient elution, wherein the specific mean-degree-of-polymerization procyanidin extract is mainly composed of (+)-catechinic acid, (-)-epicatechin and (-)-epicatechin gallate. The specific mean-degree-of-polymerization procyanidin extract can be used for preventing and treating diarrhea caused by multiple factors.

Description

technical field [0001] The invention belongs to the technical field of medicine, and in particular relates to a grape seed specific average polymerization degree proanthocyanidin extract and its application in the preparation of antidiarrheal medicines. Background technique [0002] Procyanidins (PC) belong to proanthocyanidins, which is a general term for a large class of condensation polymers of flavan-3-alcohol derivatives that widely exist in nature. named after the pigment. At the same time, due to the tannin properties exhibited by its polymers, it is also called condensed tannins. According to the position and number of hydroxyl groups on the aromatic ring of flavanol monomers, proanthocyanidins can be divided into proanthocyanidins, proanthocyanidins and procyanidins, among which proanthocyanidins are the most common type of proanthocyanidins. Natural proanthocyanidins widely exist in various plants, such as grapes, hawthorn, ginkgo, birch, strawberries, cranberrie...

Claims

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Application Information

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IPC IPC(8): A61K36/87A61K31/353A61K9/30A61K9/16A61K9/02A61P1/12
CPCA61K36/87A61K9/02A61K9/1623A61K9/1652A61K9/282A61K31/353A61K47/10A61K2236/331A61K2236/333A61K2236/51A61K2236/53A61K2300/00
Inventor 单淇侯文彬郝春华王维亭周福军华洁王淼
Owner TIANJIN INSTITUTE OF PHARMA RESEARCH
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