Broad-spectrum substituted urea herbicide degradation bacterium and amidohydrolase gene and application

A herbicide amide, amido hydrolase technology, applied in hydrolase, application, genetic engineering and other directions, can solve environmental problems, crops, phytotoxicity, serious problems, etc.
CN105779477AActive Publication Date: 2016-07-20NANJING AGRICULTURAL UNIVERSITY
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
NANJING AGRICULTURAL UNIVERSITY
Publication Date
2016-07-20

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Abstract

The invention discloses a broad-spectrum substituted urea herbicide degradation bacterium and amidohydrolase gene and application and provides an amidohydrolase gene of a substituted urea herbicide and hydrolase protein induced by the gene.The total length of the gene is 1377 bp, the G+C content is 63.5%, 458 amino acids are encoded, and the gene is a new amidohydrolase gene resource having a broad-spectrum hydrolysis function on the substituted urea herbicide, 30 mg / L N-methoxyl-N-methyl substituted urea herbicide (linuron) and N,N-dimethyl substituted urea herbicide (diuron, chlortoluron and fluometuron) can be completely degraded within 24 h, and produced enzymic preparations can be used for removing substituted urea herbicide residues on soil, water and crops.The gene can be used for cultivation of transgenic crops resisting substituted urea herbicide.
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Description

technical field

[0001] The invention belongs to the field of applied environmental microorganisms, and relates to broad-spectrum substituted urea herbicide-degrading bacteria and amidohydrolase genes and applications. Background technique

[0002] Although the use of herbicides has improved the production efficiency of agriculture, it has brought serious environmental problems and crop phytotoxicity problems at the same time. Substituted urea herbicides have been produced commercially for more than 50 years and are among the most widely used important herbicides worldwide. Due to its extensive repeated use and relatively high water solubility, it is often detected in various polluted habitats and agricultural by-products, and herbicide crop phytotoxicity also occurs from time to time. Therefore, replacing urea herbicide residues has become an urgent problem to be solved in ecological environment protection and crop phytotoxicity relief. The method of microbial remediation ...

Claims

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