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Saccharomyces cerevisiae degrading zearalenone toxins and application thereof

A technology of zearalenone and yeast, which is applied to the application field of Saccharomyces cerevisiae in the field of feed and food safety, can solve problems such as environmental pollution and impact on the application of biological control technology, and achieve avoidance of harm to humans, significant economic benefits and Social benefit, high safety effect

Inactive Publication Date: 2016-07-27
JIANGSU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Adsorbent adsorption is also a method that can effectively remove ZEA pollution, but the adsorption method will also adsorb nutrients in food while adsorbing toxins, and since the toxins have not been degraded, it will pollute the environment
Commonly used chemical methods for removing ZEA include ozone treatment, hydrogen peroxide treatment, and sodium carbonate immersion, etc., but the addition of chemical reagents will introduce many uncertain factors, and the potential toxicity of new products needs to be further studied
[0006] With the development of biological detoxification technology, the use of yeast to control the pollution of ZEA in grain and its by-products shows a good application prospect, but the relevant research is still in its infancy in the international scope, which affects the biological control technology in ZEA control. Applications

Method used

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  • Saccharomyces cerevisiae degrading zearalenone toxins and application thereof
  • Saccharomyces cerevisiae degrading zearalenone toxins and application thereof
  • Saccharomyces cerevisiae degrading zearalenone toxins and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Embodiment 1: the screening of the yeast that degrades ZEA, specific implementation steps are as follows:

[0028] 1. Isolation of yeast single colonies from the environment

[0029] Take mulberries, squeeze the juice, take 1ml of the juice, add it to a centrifuge tube filled with 10ml of sterile water, shake and mix well, take the mixed solution, and do gradient dilution (10 -4 , 10 -5 , 10 -6 , 10 -7 ), spread on NYDA medium plate, and culture at 28°C for 20-36h. Pick the dominant yeast single colony and purify and culture it on the NYDA plate. The isolated and purified strains were planted on NYDA slopes, cultured at 28°C for 48 hours, and stored in a refrigerator at 4°C for short-term storage.

[0030] 2. ZEA determination method

[0031] Adopt the method (HPLC-FLD) of high performance liquid phase-fluorescence detector to detect ZEA, the condition is as follows, chromatographic column: Agilent C 18 Column (5.0μm, 150.0mm×4.6mm); mobile phase: acetonitrile: 1...

Embodiment 2

[0039] Example 2: Microbiological properties of Saccharomyces cerevisiae 912

[0040] The above-mentioned strain 912 was identified as Saccharomyces cerevisiae through morphological culture, physiological and biochemical characteristics tests, and nucleotide sequence analysis of small subunit 5.8SrDNA and internal transcriptional spacer ITS1 and ITS2 regions.

[0041] The bacterial strain Saccharomyces cerevisiae 912 bacterial strain of the present invention (has applied for Chinese invention patent, application number 201310648466.8, title of invention: Saccharomyces cerevisiae, application and method of use in fruit postharvest disease control, application date: December 04, 2013) has the following microorganisms Academic Features:

[0042] 1. Morphological characteristics

[0043] (1) Cultured on a YEPD solid medium plate (yeast powder 10g, peptone 20g, glucose 20g, distilled water 1000mL, agar 20g, pH 6.0, 115°C damp heat sterilization for 20min) at 28°C for 48h, the colo...

Embodiment 3

[0051] Example 3: Optimization of ZEA Degradation Conditions by Saccharomyces cerevisiae 912

[0052] By studying the effect of Saccharomyces cerevisiae 912 on the degradation of ZEA under the conditions of bacterial concentration, culture temperature, shaker speed, medium pH and different initial concentrations of ZEA, it was determined that the optimal condition for its degradation of ZEA was that the initial concentration of yeast was 10 8 CFU / mL, pH 6, shaker incubation temperature 28°C, rotation speed 180rpm, and with the increase of the initial concentration of ZEA, the time required for complete degradation prolongs. When the initial concentration of ZEA was 3 μg / mL, the yeast’s degradation rate was 100% after 36 h; 100%; when the initial concentration of ZEA was 8μg / mL and 10μg / mL, the degradation rates of ZEA were 95% and 84% after 72h.

[0053] The results show that Saccharomyces cerevisiae 912 of the present invention can continuously degrade the ZEA standard produ...

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Abstract

The present invention discloses saccharomyces cerevisiae degrading zearalenone (ZEA) toxins and an application thereof, and belongs to the field of biotechnology. The optimum conditions of the saccharomyces cerevisiae for ZEA degradation are as follows: initial concentration of the saccharomyces cerevisiae is 10^8 CFU / mL, pH is 6, a shaker culture temperature is 28 DEG C and a speed is 180 rpm. The saccharomyces cerevisiae can efficiently degrade ZEA toxins with a degradation rate up to 100% and can significantly inhibit the growth of ZEA toxin produced fusarium graminearum. Besides, when the initial concentration of ZEA is 5 [mu]g / mL, the degradation rate of the saccharomyces cerevisiae to the ZEA within 24 h is maximum and the saccharomyces cerevisiae can completely degrade the ZEA toxins to be non-toxic products at 48 h. The used saccharomyces cerevisiae can replace chemical fungicides and is applied in fruit postharvest disease prevention and control, avoids the hazards of the chemical fungicides on human, and has significant economic and social benefits.

Description

technical field [0001] The invention belongs to a method for biodegrading toxins, and in particular relates to the application of Saccharomyces cerevisiae in the fields of feed and food safety. Background technique [0002] Zearalenone (Zearalenone, ZEA), also known as F-2 toxin, was originally isolated and purified from moldy corn by Stob et al. in 1962, mainly from Fusarium graminearum (F. F. moniliforme), yellow Fusarium (Fusarium culmorum), pink Fusarium (Fusarium roseum), equiseti (Fusarium equiseti) and three-line Fusarium (Fusarium tricinctum) and other Fusarium fungi. ZEA is a non-steroidal mycotoxin with estrogen action, its action strength is about 1 / 10 of estrogen, but its action time is much longer than estrogen. ZEA has a variety of toxicities, including reproductive toxicity, cytotoxicity, genotoxicity, immunotoxicity, and carcinogenicity. [0003] ZEA pollution is quite common all over the world, and widely exists in grains, animal feed and agricultural by-p...

Claims

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Application Information

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IPC IPC(8): A23B7/155A23B9/28A23L3/3571C12N1/18C12R1/865
CPCA23B7/155A23B9/28A23L3/3571A23V2002/00C12N1/18A23V2200/10A23V2250/76
Inventor 张红印窦勇张晓云任晓锋董曼佳程洋洋
Owner JIANGSU UNIV
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