Heat-resisting protective agent for TGEV and PEDV bigeminy freeze-drying live vaccine and preparing method and application of heat-resisting protective agent

A heat-resistant protective agent, TGEV technology, applied in the field of vaccines, can solve the problem of lack of heat-resistant protective agent

Active Publication Date: 2016-07-27
TIANJIN RINGPU BIO TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The present invention aims at the technical defects of the prior art, and provides a heat-resistant protective agent for TGEV and PEDV dual freeze-dried live vaccines to solve the lack of a heat-resistant protective agent for this specific vaccine in the prior art technical issues

Method used

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  • Heat-resisting protective agent for TGEV and PEDV bigeminy freeze-drying live vaccine and preparing method and application of heat-resisting protective agent
  • Heat-resisting protective agent for TGEV and PEDV bigeminy freeze-drying live vaccine and preparing method and application of heat-resisting protective agent
  • Heat-resisting protective agent for TGEV and PEDV bigeminy freeze-drying live vaccine and preparing method and application of heat-resisting protective agent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Embodiment 1 (preparation and inspection of porcine transmissible gastroenteritis, epidemic diarrhea dual heat-resistant protective agent live vaccine)

[0037] 1. Preparation of heat-resistant protective agent

[0038] 1.1 Weigh macromolecular substances such as gelatin and polyvinylpyrrolidone, dissolve them in water for injection in proportion, and sterilize under high pressure at 115°C for 20 minutes, as liquid A.

[0039] 1.2 Dissolve small molecular substances such as lactose, hydrolyzed milk protein, trehalose, L-arginine, and sodium glutamate in water for injection in proportion, and filter and sterilize with a 0.22 μm filter membrane as liquid B.

[0040] 1.3 Mix the above-mentioned liquid A and liquid B at a ratio of 1:1 to obtain a heat-resistant protective agent for porcine transmissible gastroenteritis and epidemic diarrhea dual live vaccine, wherein the proportions of each component are gelatin 2%, PVPK305%, lactose 6%, hydrolyzed milk protein 1%, trehalo...

Embodiment 2

[0063] Embodiment 2 (preparation and inspection of porcine transmissible gastroenteritis, epidemic diarrhea dual heat-resistant protective agent live vaccine)

[0064] 1. Preparation of heat-resistant protective agent

[0065] 1.1 Dissolve macromolecular substances such as gelatin and polyvinylpyrrolidone in proportion with ultrapure water, and sterilize under high pressure at 116°C for 20 minutes, as liquid A;

[0066] 1.2 Dissolve lactose, hydrolyzed milk protein, trehalose, L-arginine, sodium glutamate and other small molecular substances in ultrapure water in proportion, and filter and sterilize with a 0.22 μm filter membrane as liquid B;

[0067] 1.3 Mix the above liquid A and liquid B at a ratio of 1:1 to obtain the heat-resistant protective agent for porcine transmissible gastroenteritis and epidemic diarrhea dual live vaccine, wherein the proportions of each component are gelatin 1.5%, gelatin PVPK306%, lactose 8%, hydrolyzed milk protein 1.5%, trehalose 2%, L-arginin...

Embodiment 3

[0089] Example 3 Preparation and inspection of porcine transmissible gastroenteritis and epidemic diarrhea dual heat-resistant protective agent live vaccine

[0090] 1. Preparation of heat-resistant protective agent

[0091] 1.1 Dissolve macromolecular substances such as gelatin and polyvinylpyrrolidone in proportion with ultrapure water, and sterilize under high pressure at 121°C for 15 minutes, as liquid A;

[0092] 1.2 Dissolve small molecular substances such as lactose, hydrolyzed milk protein, trehalose, L-arginine, and sodium glutamate in proportion to ultrapure water, and filter and sterilize with a 0.22 μm filter membrane as liquid B;

[0093] 1.3 Mix the above-mentioned liquid A and liquid B at a ratio of 1:1 to obtain a heat-resistant protective agent for porcine transmissible gastroenteritis and epidemic diarrhea dual live vaccine, wherein the proportions of each component are gelatin 1%, gelatin 1%, PVPK308%; of which lactose 4%, hydrolyzed milk protein 3%, trehal...

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Abstract

The invention provides a heat-resisting protective agent for a TGEV and PEDV bigeminy freeze-drying live vaccine and a preparing method and application of the heat-resisting protective agent.The heat-resisting protective agent is prepared from, by weight, 1-3% of gelatin, 2-10% of lactose, 0.5-3% of lactoalbumin hydrolysate, 1-6% of trehalose, 0.1-3% of L-arginine, 1-3% of sodium glutamate, 2-8% of polyvinyl pyrrolidone and the balance water.Compared with a heat-resisting protective agent with a conventional saccharose gelatin ingredient, the TGEV and PEDV bigeminy freeze-drying live vaccine prepared from the heat-resisting protective agent is safe, effective, high in stability, low in virus antigen freeze-drying loss ratio, good in heat resistance and long in retention period.Due to the fact that a proper freeze-drying technology is matched with the heat-resisting protective agent, the protection effect of the heat-resisting protective agent on antigens is further promoted, and the freeze-drying efficiency is improved.After the prepared vaccine is placed for 14 days at 37 DEG C, the decrease of the virus content is not larger than 0.5 titer; after the prepared vaccine is stored for 30 months at 2-8 DEG C, the freezing-dried appearance is still maintained, and the virus content is still larger than or equal to 105.0 TCID50; the vacuum degree, the remaining moisture and other detection items of the vaccine are all qualified.

Description

technical field [0001] The invention relates to the technical field of vaccines, and further relates to vaccine preparation technology, in particular to a heat-resistant protective agent for TGEV and PEDV dual freeze-dried live vaccines, its preparation method and application. Background technique [0002] Porcine transmissible gastroenteritis is a highly contagious intestinal infectious disease caused by porcine transmissible gastroenteritis virus (TGEV), which causes vomiting, severe diarrhea, dehydration and high mortality in piglets under 2 weeks of age. main features. Porcine epidemic diarrhea is an acute contact intestinal infectious disease of pigs caused by porcine epidemic diarrhea virus (Porcineepidemicdiarrheavirus, PEDV), characterized by vomiting, diarrhea, loss of appetite and dehydration, pigs of all ages are prone to feel. Porcine transmissible gastroenteritis and porcine epidemic diarrhea are the main viral diseases that cause porcine diarrhea. The clinica...

Claims

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Application Information

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IPC IPC(8): A61K47/42A61K47/26A61K47/32A61K47/36A61K47/18A61K39/225A61K39/215A61K9/19A61P31/14
CPCA61K9/19A61K39/12A61K47/183A61K47/26A61K47/32A61K47/36A61K47/42A61K2039/552A61K2039/70C12N2770/20034C12N2770/24334
Inventor 丁旭娜吕茂杰杨保收付旭彬梁武李守军
Owner TIANJIN RINGPU BIO TECH
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