A kind of composite acellular matrix hydrogel and preparation method thereof
An acellular matrix, hydrogel technology, applied in medical science, prosthesis and other directions, can solve the problems of excessively fast migration speed and insufficient structural strength of effectively added components, achieve a good three-dimensional space structure, and the preparation process is simple and feasible. The effect of improving utilization
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Embodiment 1
[0050] A method for preparing a composite acellular matrix hydrogel. The acellular matrix material obtained from the nerve is prepared, freeze-dried, then freeze-ground, and crushed into powder; 100 mg of the acellular matrix powder is dissolved in 10 mL of 0.01mol / L hydrochloric acid Add 10 mg of pepsin to the solution, place it on a shaker for digestion for about 48 hours to form a decellularized matrix pregel solution, add about 1 / 10 of the volume of the decellularized matrix pregel solution NaOH, wherein the concentration of NaOH solution is 0.01mol / L, then add 10×PBS about 1 / 9 of the volume of the acellular matrix pregel solution, wherein the concentration of the PBS solution is 0.01mol / L, and then add vascular endothelial growth factor, wherein the vascular endothelial growth factor and the acellular matrix The mass ratio is 0.025:100 to form an acellular matrix gel precursor solution.
[0051] Before the gel is formed, the fiber diameter prepared by electrospinning is ...
Embodiment 2
[0053] A method for preparing a composite acellular matrix hydrogel. The acellular matrix material obtained from the spinal cord is prepared, freeze-dried, then freeze-ground, and crushed into powder; 100 mg of the acellular matrix powder is dissolved in 10 mL of 0.01 mol / L hydrochloric acid Add 10 mg of pepsin to the solution, place it on a shaker for digestion for about 48 hours to form a decellularized matrix pregel solution, add about 1 / 10 of the volume of the decellularized matrix pregel solution NaOH, wherein the concentration of NaOH solution is 0.01mol / L, then add 10×PBS about 1 / 9 of the volume of the acellular matrix pregel solution, wherein the concentration of the PBS solution is 0.01mol / L, then add insulin, wherein the mass ratio of insulin to the acellular matrix is 0.05:100, Form the acellular matrix gel precursor solution.
[0054] Before the gel was formed, the above-mentioned acellular matrix precursor solution was coated on the fiber prepared by the three-...
Embodiment 3
[0056] A method for preparing a composite acellular matrix hydrogel. The acellular matrix material obtained from the tendon is prepared, freeze-dried, then freeze-ground, and crushed into powder; 100 mg of the acellular matrix powder is dissolved in 10 mL of 0.01mol / L hydrochloric acid Add 10 mg of pepsin to the solution, place it on a shaker for digestion for about 48 hours to form a decellularized matrix pregel solution, add about 1 / 10 of the volume of the decellularized matrix pregel solution NaOH, wherein the concentration of the NaOH solution is 0.01mol / L, then add 10×PBS about 1 / 9 of the volume of the acellular matrix pregel solution, wherein the concentration of the PBS solution is 0.01mol / L, then add aspirin, wherein the mass ratio of aspirin to the acellular matrix is 1:20, Form the acellular matrix gel precursor solution.
[0057] Before the gel was formed, the above-mentioned acellular matrix precursor solution was coated on the fiber prepared by the three-dimens...
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