Preparation method of polygonatum odoratum polysaccharide
A polysaccharide from Polygonatum polysaccharide, Polygonatum polysaccharide technology, applied in the field of polysaccharide preparation of Polygonatum polysaccharide, can solve the problems of polysaccharide product quality instability, high protein and pigment content, high extraction temperature, etc. The effect of mild reaction conditions
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example 1
[0020] First weigh 30g Polygonatum odoratum stems, wash them with deionized water for 3 times, then use a pulverizer to grind them, then add the pulverized Polygonatum odoratum powder into the enzymatic hydrolysis tank, and at the same time add 600mL deionized Water, 100mL pH 7.0 phosphate buffer solution, stir for 20min, then add 1g of pectinase, 2g of cellulase, 1g of hemicellulase to the enzymolysis tank, control the temperature in the enzymolysis tank to 45°C, stir for 4h After the reaction is over, raise the temperature to 100°C, heat for 20 minutes, and then cool down to room temperature to obtain the Enzymatic Hydrolyzate of Polygonatum Polygonatum; then put the Enzymatic Hydrolyzate of Polygonatum Polygonatum in a centrifuge at 6000r / min for 20min, and discard the impurities in the lower layer , to obtain the enzymolysis supernatant of Polygonatum odoratum, then add the obtained enzymolysis supernatant to the beaker, add 2g of ammonium sulfate to the beaker, stir for 10...
example 2
[0023] First, weigh 35g Polygonatum odoratum stems, wash them with deionized water for 4 times, then use a pulverizer to grind them, then add the pulverized Polygonatum odoratum powder into the enzymatic hydrolysis tank, and at the same time add 650mL deionized Water, 150mL phosphate buffer solution with a pH of 7.1, stirred for 25min, then added 2g of pectinase, 3g of cellulase, and 2g of hemicellulase to the enzymolysis tank in turn, controlled the temperature in the enzymolysis tank to 50°C, and stirred for 4.5 h, when the reaction is over, raise the temperature to 105°C, heat for 25 minutes, then cool down to room temperature to obtain Polygonatum odoratum enzymolysis solution; then put the Polygonatum odoratum enzymolysis solution in a 7000r / min centrifuge for centrifugation for 25min, discard the lower layer Impurities were obtained by enzymolysis supernatant of Polygonatum odoratum, and then the obtained enzymolysis supernatant was added to a beaker, 3g of ammonium sulfa...
example 3
[0026] First weigh 40g Polygonatum odoratum stem, wash it with deionized water for 5 times, then use a pulverizer to grind it, add the pulverized Polygonatum odoratum powder into the enzymatic hydrolysis tank, and at the same time add 700mL deionized Water, 200mL pH 7.2 phosphate buffer solution, stir for 30min, then add 3g of pectinase, 4g of cellulase, 3g of hemicellulase to the enzymolysis tank in turn, control the temperature in the enzymolysis tank to 55°C, stir for 5h After the reaction is over, raise the temperature to 110°C, heat for 30 minutes, and then cool down to room temperature to obtain Polygonatum odoratum enzymolysis solution; then put the Polygonatum odoratum enzymolysis solution in a centrifuge at 8000r / min for 30 minutes, and discard the impurities in the lower layer , to obtain the enzymolysis supernatant of Polygonatum odoratum, then add the obtained enzymolysis supernatant to the beaker, add 4g of ammonium sulfate to the beaker, stir for 20min, let stand ...
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