Method for efficiently producing cellulose based on preparation of mixture through glucose glucoside conversion reaction

Abstract

The invention discloses a method for efficiently producing cellulose based on preparation of a mixture through glucose glucoside conversion reaction. According to the method, glucose or syrup containing glucose is adopted for preparing a sophorose-containing mixture through glucoside conversion reaction under catalysis of beta-glycosidase, the sophorose-containing mixture is adopted as a carbon source and an inductor for culturing Trichoderma reesei, and the cellulose is efficiently produced, wherein in the fermentation tank culture process, through the batch-type feeding of the mixture, the glucose concentration in the fermentation liquor is controlled to be 0.05-0.30 g / L, so that the mycelial growth is facilitated, but the biosynthesis of the cellulose is not inhibited; after the culture is carried out for 144h, the activity of the cellulose in the fermentation liquor exceeds 90.0FPU / mL, and the production intensity of the cellulose in the fermentation tank exceeds 625.0FPU / L / h. Due to the high activity and high production intensity of the cellulose, the investment on the production equipment and the energy consumption during the fermentation process of the cellulose are obviously saved, and the production cost of the cellulose is reduced.

Description

technical field [0001] The invention relates to the field of bioengineering and technology, in particular to a method for efficiently producing cellulase based on a glucose transglycoside reaction preparation mixture. Background technique [0002] Lignocellulosic biomass is the most abundant renewable resource on the earth. Bioenergy and bio-based chemicals produced through biorefinery, the carbon dioxide produced during use can be absorbed by plant photosynthesis, and does not produce obvious greenhouse gas effects. However, biorefineries produce bioenergy and biobased chemicals at a cost that cannot compete with similar products based on mineral resources such as oil and coal. Cellulose is the carbohydrate with the highest content in lignocellulosic biomass resources. Cellulose must be hydrolyzed into glucose before it can be used as the basic raw material for microbial fermentation to produce bioenergy and biobased chemicals. Although the cellulose component can be hydro...

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to overcome the high cost and difficult control of adding cellulose insoluble inducers in the cellulase fermentation production process existing in the prior art and the low cellulase enzyme activity and low production intensity in the fermentation process of soluble inducers such as lactose Highlight the problem and provide a method for the efficient production of cellulase based on the preparation mixture of glucose transglycoside reaction

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