Method for efficiently producing cellulose based on preparation of mixture through glucose glucoside conversion reaction

A technology of cellulase and glucose, which is applied in the field of high-efficiency production of cellulase based on the preparation of mixtures based on glucose transglycoside reaction, can solve the problems of low enzyme activity, high cost, and low production intensity of cellulase, so as to reduce production costs and save The effect of process energy consumption and shortening fermentation time

Inactive Publication Date: 2016-09-07
SHANGHAI JIAO TONG UNIV
View PDF10 Cites 6 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to overcome the high cost and difficult control of adding cellulose insoluble inducers in the cellulase fermentation production process existing in the prior art and the low cellulase enzyme activity

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for efficiently producing cellulose based on preparation of mixture through glucose glucoside conversion reaction
  • Method for efficiently producing cellulose based on preparation of mixture through glucose glucoside conversion reaction

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Example 1, β-glucosidase catalyzed glucose transglycoside reaction to prepare MGD

[0031] Prepare 600g / L glucose solution, add 20IU β-glucosidase produced by Xiasheng Industrial Group Co., Ltd. per gram of glucose, incubate at pH 4.8 and 65°C for 72 hours, then boil the glucose solution for 5 minutes to inactivate β-glucosidase Then the soluble inducer MGD can be prepared. It has been determined that MGD contains 410.3g / L of glucose and 13.7g / L of sophorose.

Embodiment 2

[0032] Example 2, batch feeding MGD culture T.reesei RUT C30 to efficiently produce cellulase

[0033] Batch fed-batch culture T. reesei RUT C30 production cellulase process as follows figure 1 shown. The oxygen from 1 in the oxygen cylinder and the air in the air compressor 2 are regulated by the regulating valve 3 and metered by the flow meter 4 to mix in an appropriate ratio and then enter the gas humidifier 5 for humidification, then pass into the fermenter 6, and The dissolved oxygen (DO) detection system 8 is linked to control the DO in the fermentation broth to not be less than 20%, so as to meet the requirements of mycelium growth and cellulase biosynthesis. The temperature detection system 7, the circulating water regulating valve 12, the circulating water tank 10 and the circulating water pump 11 form a temperature regulating control system. The pH detection system 9, the ammonia water storage tank 13 and the peristaltic pump 16 constitute a pH adjustment control s...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a method for efficiently producing cellulose based on preparation of a mixture through glucose glucoside conversion reaction. According to the method, glucose or syrup containing glucose is adopted for preparing a sophorose-containing mixture through glucoside conversion reaction under catalysis of beta-glycosidase, the sophorose-containing mixture is adopted as a carbon source and an inductor for culturing Trichoderma reesei, and the cellulose is efficiently produced, wherein in the fermentation tank culture process, through the batch-type feeding of the mixture, the glucose concentration in the fermentation liquor is controlled to be 0.05-0.30 g/L, so that the mycelial growth is facilitated, but the biosynthesis of the cellulose is not inhibited; after the culture is carried out for 144h, the activity of the cellulose in the fermentation liquor exceeds 90.0FPU/mL, and the production intensity of the cellulose in the fermentation tank exceeds 625.0FPU/L/h. Due to the high activity and high production intensity of the cellulose, the investment on the production equipment and the energy consumption during the fermentation process of the cellulose are obviously saved, and the production cost of the cellulose is reduced.

Description

technical field [0001] The invention relates to the field of bioengineering and technology, in particular to a method for efficiently producing cellulase based on a glucose transglycoside reaction preparation mixture. Background technique [0002] Lignocellulosic biomass is the most abundant renewable resource on the earth. Bioenergy and bio-based chemicals produced through biorefinery, the carbon dioxide produced during use can be absorbed by plant photosynthesis, and does not produce obvious greenhouse gas effects. However, biorefineries produce bioenergy and biobased chemicals at a cost that cannot compete with similar products based on mineral resources such as oil and coal. Cellulose is the carbohydrate with the highest content in lignocellulosic biomass resources. Cellulose must be hydrolyzed into glucose before it can be used as the basic raw material for microbial fermentation to produce bioenergy and biobased chemicals. Although the cellulose component can be hydro...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12N9/42
CPCC12N9/2437C12Y302/01004
Inventor 白凤武赵心清李勇昊
Owner SHANGHAI JIAO TONG UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap