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Broiler feed on basis of lactobacillus johnsonii BS15 and method for preparing broiler feed

A technology of Lactobacillus johnsonii and broiler feed, which is applied in the field of broiler breeding, can solve problems such as drug residues, and achieve the effects of improving the structure of bacteria, improving the intestinal environment and ensuring food health.

Inactive Publication Date: 2016-09-21
SICHUAN AGRI UNIV
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Problems solved by technology

[0003] The purpose of the present invention is to provide a broiler feed based on Lactobacillus johnsonii BS15 and its preparation method, aiming to solve the problem of using BS15 to improve the quality of broiler chickens with subclinical NE disease, and to make up for the technical gap in the field of food safety and antibiotics. drug residue problem

Method used

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  • Broiler feed on basis of lactobacillus johnsonii BS15 and method for preparing broiler feed
  • Broiler feed on basis of lactobacillus johnsonii BS15 and method for preparing broiler feed
  • Broiler feed on basis of lactobacillus johnsonii BS15 and method for preparing broiler feed

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preparation example Construction

[0024] like figure 1 As shown, the preparation method of the broiler feed based on Lactobacillus johnsonii BS15 according to the embodiment of the present invention comprises the following steps:

[0025] S101: Take out the Lactobacillus johnsonii BS15 strain stored in the Microbial Culture Collection Center, thaw and thaw the strain at 25°C, and inoculate 1-2 loops in the modified MRS solid medium (modified MRS medium formula: Peptone 10.00g / L, beef extract 5.00g / L, yeast extract 4.00g / L, glucose 20.00g / L, Tween-80 1.00mL / L, sodium acetate 5.00g / L, diammonium citrate 0.20g, phosphoric acid Dipotassium Hydrogen (K 2 HPO 4 ) 2.00g / L, magnesium sulfate (MgSO 4 ·7H 2 0) 0.20g / L, manganese sulfate (MnSO 4 ·10H 2 0) 0.05g / L, solid medium needs to add 17.00g agar, add sterilized water to 1000mL, adjust pH to 6.2, sterilize at 121°C for 20min), and place it in a biochemical incubator at 36-37°C for constant temperature cultivation for 12h;

[0026] S102: pick the obtained sing...

Embodiment 1

[0035] Embodiment 1 is to the detection of chicken basic meat quality index (chicken specific gravity, meat color, pH value, water retention, tenderness)

[0036] After the chickens were slaughtered, the chest muscles and leg muscles were stripped to calculate the muscle specific gravity; the left chest muscles and leg muscles were taken, and the CR-300 portable colorimeter was used to detect the L* value (brightness value) and a* value at the thickest and regular part of the muscle. (redness value) and b* value (yellowness value), the arithmetic mean of three data is taken for each chicken; use the PH-STAR portable pH tester to perform puncture detection on the same part of the ipsilateral chest muscle and leg muscle, Take three data for each chicken and take the arithmetic mean as the criterion, record the pH value of the pectoralis muscle after 40min and 24h; use the method of measuring drip loss to measure the muscle water retention capacity, peel off the entire right pecto...

Embodiment 2

[0042] Example 2: Detection of Shelf Cycle (ie Antioxidant Ability) of Chicken

[0043] On the 42nd day of the experiment, 12 animals were randomly selected from each group, and the left pectoralis major and leg muscles were frozen and stored at -20°C. The muscle was made into 10% tissue homogenate, and the activity of SOD, GSH-Px, CAT and the content of MDA in the muscle were detected using a kit (purchased from Nanjing Jiancheng Biotechnology Co., Ltd.).

[0044] Table 2. Lactobacillus johnsonii BS15 can prolong the shelf life of subclinical NE broiler muscle (improves antioxidant capacity)

[0045]

[0046] SOD = superoxide dismutase; CAT = catalase; GSH-Px = glutathione peroxidase; MDA = malondialdehyde

[0047] a,b The difference between the superscript numbers in each row is significant. (P<0.05) The results are presented in the form of mean ± standard error.

[0048] The results show that Lactobacillus johnsonii BS15 can prolong the shelf life of subclinical NE br...

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Abstract

The invention discloses broiler feed on the basis of lactobacillus johnsonii BS15 and a method for preparing the broiler feed. The broiler feed on the basis of the lactobacillus johnsonii BS15 contains 1*10<8>-1*10<9> cfu / kg of the lactobacillus johnsonii BS15. The service time of the boiler feed starts from one day after chicks are hatched to the broiler slaughtering time; the broiler feed and feed are mixed with each other and then are fed to broilers. The broiler feed and the method have the advantages that the problem of drug residues due to antibiotics can be solved, and gaps in the field after the antibiotics are forbidden can be filled; the intestinal tracts of the broilers can be regulated, the purpose of allowing the broilers to healthfully grow can be achieved, and growth promotion and disease prevention functions are integrated by a novel prevention and control process; the broiler feed is used for assisting in establishing healthful digestive systems of the broilers, and accordingly deterioration of the meat quality of the broilers can be prevented when sub-clinical necrotic enteritis occurs.

Description

technical field [0001] The invention belongs to the technical field of broiler breeding, in particular to a broiler feed based on Lactobacillus johnsonii BS15 and a preparation method thereof. Background technique [0002] Necrotic enteritis (NE) is one of the most concerning problems in broiler breeding industry, which is mainly caused by Clostridium perfringens (CP). CP is sensitive to antibiotics. In some developed countries, the incidence of NE is increasing due to the prohibition of antibiotics as growth promoters. Taking the United States as an example, the economic loss caused by NE has reached 3 billion US dollars per year, with an average loss of 0.05 US dollars per broiler. In addition, the number of food poisoning cases caused by type A CP has ranked second in the United States, Britain and other countries. Broiler NE is divided into clinical and subclinical types, which can damage intestinal mucosa and damage intestinal health. Clinical NE mainly occurs in chic...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K50/75A23K10/18
Inventor 倪学勤曾东王鹤松舒刚景波周梦佳潘康成
Owner SICHUAN AGRI UNIV
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