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A broiler feed based on Lactobacillus johnsonii bs15 and preparation method thereof

A technology of Lactobacillus johnsoni and broiler feed, applied in the field of broiler breeding, can solve problems such as drug residues, achieve the effect of improving the structure of the flora, improving the environment in the intestinal tract, and ensuring the health of food

Inactive Publication Date: 2020-04-10
SICHUAN AGRI UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The purpose of the present invention is to provide a broiler feed based on Lactobacillus johnsonii BS15 and its preparation method, aiming to solve the problem of using BS15 to improve the quality of broiler chickens with subclinical NE disease, and to make up for the technical gap in the field of food safety and antibiotics. drug residue problem

Method used

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  • A broiler feed based on Lactobacillus johnsonii bs15 and preparation method thereof
  • A broiler feed based on Lactobacillus johnsonii bs15 and preparation method thereof
  • A broiler feed based on Lactobacillus johnsonii bs15 and preparation method thereof

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preparation example Construction

[0024] Such as figure 1 Shown, the preparation method of the broiler feed based on Lactobacillus johnsonii BS15 of the embodiment of the present invention comprises the following steps:

[0025] S101: Take out the Lactobacillus johnsonii BS15 strains preserved from the Microbial Culture Collection Center, thaw the strains at 25°C, and inoculate 1-2 loops on the improved MRS solid medium (improved MRS medium formula: Peptone 10.00g / L, beef extract 5.00g / L, yeast extract 4.00g / L, glucose 20.00g / L, Tween-80 1.00mL / L, sodium acetate 5.00g / L, diammonium citrate 0.20g, phosphoric acid Dipotassium Hydrogen (K 2 HPO 4 ) 2.00g / L, magnesium sulfate (MgSO 4 ·7H 2 0) 0.20g / L, manganese sulfate (MnSO 4 10H 2 0) 0.05g / L, add 17.00g of agar to the solid medium, add sterilized water to 1000mL, adjust the pH to 6.2, sterilize at 121°C for 20min), place in a biochemical incubator at 36-37°C for 12h;

[0026] S102: Pick the obtained single colony, inoculate it into the improved MRS liquid...

Embodiment 1

[0035] Embodiment 1 is to the detection of chicken basic meat quality index (chicken specific gravity, meat color, pH value, water retention capacity, tenderness)

[0036] After slaughtering the chicken, peel off the breast muscle and leg muscle, and calculate the muscle specific gravity; take the left breast muscle and leg muscle, and use a CR-300 portable colorimeter to detect the L* value (brightness value) and a* value of the thickest and most regular part of the muscle (redness value) and b*value (yellowness value), take three data from each chicken and take the arithmetic mean as the criterion; use the PH-STAR portable pH tester to puncture the same part of the ipsilateral breast muscle and leg muscle, Take the data three times from each chicken and take the arithmetic mean as the standard, record the pH value of the pectoral muscle after 40 minutes and 24 hours; measure the water retention capacity of the muscle by measuring the dripping water loss, peel off the whole pe...

Embodiment 2

[0042] Embodiment 2: detection of chicken shelf life (ie antioxidant capacity)

[0043] On the 42nd day of the experiment, 12 animals were randomly selected from each group, and the left pectoralis major and leg muscles were frozen and stored at -20°C. The muscle was made into 10% tissue homogenate, and the activities of SOD, GSH-Px, CAT and the content of MDA in the muscle were detected using a kit (purchased from Nanjing Jiancheng Biotechnology Company).

[0044] Table 2. Lactobacillus johnsonii BS15 can prolong the shelf life of subclinical NE broiler muscle (improve antioxidant capacity)

[0045]

[0046] SOD = superoxide dismutase; CAT = catalase; GSH-Px = glutathione peroxidase; MDA = malondialdehyde

[0047] a,b The superscript numbers in each row of data are different and the difference is significant. (P<0.05) The results are presented in the form of mean ± standard error.

[0048] The results showed that Lactobacillus johnsonii BS15 can prolong the shelf life o...

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Abstract

The invention discloses broiler feed on the basis of lactobacillus johnsonii BS15 and a method for preparing the broiler feed. The broiler feed on the basis of the lactobacillus johnsonii BS15 contains 1*10<8>-1*10<9> cfu / kg of the lactobacillus johnsonii BS15. The service time of the boiler feed starts from one day after chicks are hatched to the broiler slaughtering time; the broiler feed and feed are mixed with each other and then are fed to broilers. The broiler feed and the method have the advantages that the problem of drug residues due to antibiotics can be solved, and gaps in the field after the antibiotics are forbidden can be filled; the intestinal tracts of the broilers can be regulated, the purpose of allowing the broilers to healthfully grow can be achieved, and growth promotion and disease prevention functions are integrated by a novel prevention and control process; the broiler feed is used for assisting in establishing healthful digestive systems of the broilers, and accordingly deterioration of the meat quality of the broilers can be prevented when sub-clinical necrotic enteritis occurs.

Description

technical field [0001] The invention belongs to the technical field of broiler chicken breeding, and in particular relates to a broiler feed based on Lactobacillus johnsonii BS15 and a preparation method thereof. Background technique [0002] Necrotic enteritis (NE) is one of the most noteworthy problems in the broiler industry. It is mainly caused by Clostridium perfringens (CP). CP is sensitive to antibiotics. In some developed countries, the incidence of NE is increasing due to the prohibition of antibiotics as growth promoters. Taking the United States as an example, NE has caused economic losses of 3 billion US dollars per year, with an average loss of 0.05 US dollars per broiler chicken. In addition, the number of cases of food poisoning caused by Type A CP ranks second in the United States, Britain and other countries. Broiler NE is divided into clinical and subclinical types, both of which can damage the intestinal mucosa and impair intestinal health. Clinical NE m...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A23K50/75A23K10/18
Inventor 倪学勤曾东王鹤松舒刚景波周梦佳潘康成
Owner SICHUAN AGRI UNIV
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