Protein grafted copolymer and preparation method thereof

A technology of graft copolymer and protein, which is applied in the field of protein graft copolymer, can solve the problems that cannot meet the performance requirements of drug carrier materials, protein cannot exist stably for a long time, immunogenicity, long circulation time in the body, etc., and achieve simple Rapid preparation, long preparation cycle, complex post-processing effects

Inactive Publication Date: 2016-10-26
BEIJING CENT FOR PHYSICAL & CHEM ANALYSIS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to factors such as poor solubility and immunogenicity, the protein cannot exist stably for a long time in the body, and cannot meet the performance requirements of the drug carrier material; and the introduction of functional polymers can endow the protein with a stable structure, no immunogenicity, and a longer circulation time in the body. Long and other characteristics

Method used

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  • Protein grafted copolymer and preparation method thereof
  • Protein grafted copolymer and preparation method thereof
  • Protein grafted copolymer and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Embodiment 1: the preparation of the keratin graft polyethylene glycol (Keratin-g-PEG) of graft rate 50%

[0039] Disperse 200mg of keratin in 4mL of water, then slowly add the initiator potassium persulfate (K 2 S 2 o 8 , 22mg) and 640mg MPEGMA, to be K 2 S 2 o 8 After fully dissolving with MPEGMA, the reaction was carried out at 70°C for 24h under continuous stirring. After the reaction, a dialysis bag with a molecular weight cut-off of 7 kDa was used to dialyze in water for 24 hours, and then freeze-dried to obtain a solid keratin-g-PEG graft copolymer with a graft rate of 50%.

[0040] As can be seen from Figure 1(a), the proton peak at δ=3.75ppm in the Keratin-g-PEG NMR spectrum with a grafting rate of 50% belongs to the MPEGMA –CH 2 CH 2 The proton peak of O– indicates that the PEG chain has been successfully grafted onto the keratin backbone. By adjusting the feed ratio of MPEGMA and keratin, graft copolymers with different grafting ratios c...

Embodiment 2

[0045] Embodiment 2: the preparation of grafting rate 43% keratin graft poly-N-(2-hydroxypropyl) methacrylamide (keratin-g-PHPMA)

[0046] Disperse 200 mg of keratin in 4 mL of water, then add 4 mg of initiator 2,2'-azobisisobutyl ether hydrochloride (V-50) and 286 mg of N-(2-hydroxypropyl)methyl Acrylamide (HPMA). The reaction was then carried out at 50°C for 24 hours with constant stirring. After the reaction, the reactant was poured into a dialysis bag with a molecular weight cut-off of 7 kDa, dialyzed in water for 24 hours to remove the initiator and unreacted monomer, and the water was changed every 12 hours. After the dialysis was completed, a solid keratin-g-PHPMA graft copolymer with a graft ratio of 43% was obtained by freeze-drying. keratin-g-PHPMA in Figure 1(b) 43％ The proton peaks at 3.9 and 3.2 ppm on the H NMR spectrum are derived from proton H at e and f marked on PHPMA, indicating that PHPMA has been successfully grafted onto the keratin main chain. By...

Embodiment 3

[0051] Embodiment 3: the preparation of grafting rate 62% keratin graft poly-N-isopropylacrylamide (keratin-g-PNIPAM)

[0052] 100 mg of keratin was dispersed in 4 ml of Milli-Q water, followed by the addition of 4 mg of Initiator V-50 and 670 mg of N-isopropylacrylamide (NIPAM). The reaction was carried out at 50°C under constant stirring for 24 hours, then the reactant was poured into a dialysis bag with a molecular weight cut-off of 7kDa, and dialyzed in water for 24 hours to remove the initiator and unreacted monomer, and the water was changed every 12 hours. After the dialysis was completed, the keratin-g-PNIPAM graft copolymer with a graft rate of 53% was obtained by freeze-drying.

[0053] The proton peak at 1.2ppm on the keratin-g-PNIPAM NMR spectrum in Figure 1(c) comes from -CH on PNIPAM 3 The proton H on , the proton peak in the range of 1.5-2.5ppm is derived from the PNIPAM repeating unit -CH 2 The proton on CH-, the proton peak of 3.8ppm is attributed to...

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Abstract

The invention relates to a protein grafted copolymer. Protein is taken as a substrate, polymer side chains are grafted and polymerized on main chains of the protein, and the copolymer contains about 14.0%-94.0% by mass of the protein and about 6%-86% by mass of the polymer side chains. The invention relates to a preparation method and an application of the protein grafted copolymer.

Description

technical field [0001] The invention belongs to the field of polymer science and technology, and specifically relates to a protein graft copolymer, and further relates to its preparation method and application. Background technique [0002] Biocompatible amphiphilic polymers have attracted extensive attention in drug delivery because they can self-assemble into micelles, and the hydrophobic core of the micelles can efficiently load hydrophobic drug molecules. This is because the polymer micelles used as drug carriers can not only protect drug molecules from the influence of other substances in the body, but also have a passive targeting effect and can selectively gather at the tumor site, so as to improve the curative effect during treatment, The purpose of reducing toxic side effects. [0003] Stimuli-responsive smart polymer materials, especially stimuli-responsive polymer micelles in aqueous solution, have potential applications in slow-controlled release, drug delivery,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08G81/00C08F289/00C08F220/58C08F220/54A61K47/42A61K9/107A61L27/22
CPCA61K9/1075A61K47/42A61L27/22C08F289/00C08G81/00C08F220/58C08F220/54
Inventor 李琴梅魏晓晓沈上圯刘威刘伟丽
Owner BEIJING CENT FOR PHYSICAL & CHEM ANALYSIS
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