Method for extracting blueberry anthocyanin

A blueberry anthocyanin and extraction method technology, applied in the field of blueberry anthocyanin extraction, can solve the problems of increasing the dissolution rate of blueberry anthocyanin, difficulty in industrial production, expensive equipment, etc., achieve high medical care value, promote dissolution, The effect of wide practical application field

Inactive Publication Date: 2016-11-09
仇颖莹
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The technical problem mainly solved by the present invention: In the process of extracting anthocyanins in the current traditional method, water extraction or solvent extraction will destroy the anthocyanin structure due to high temperature, affecting its biological activity and yield, while supercritical extraction Although the method can improve the extraction rate, the equipment is expensive, the cost is high, and the status quo that is not easy for industrialized production provides a kind of blueberry fruit as raw material, crushing and beating, using a mass fraction of 40% ethanol solution as the extraction solution, and adding cellulase , extracted with the assistance of ultrasound, and then centrifuged,

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0017] Example 1

[0018] First, weigh 200g of blueberry fruit, wash and remove impurities, add 200mL of deionized water to the homogenizer for 10 minutes to break and beat, add the blueberry slurry to 2L of 40% ethanol solution, and add 30g of cellulose in a 50℃ constant temperature water bath. Enzyme, and use 200W ultrasonic ultrasound for 10 minutes, adjust the pH to 2.0 with a mass concentration of 10% hydrochloric acid solution, extract for 2 hours, place the extract in a 4000 / min centrifuge and centrifuge for 10 minutes to obtain the precipitate and supernatant. The above operation was extracted once again and the supernatant was collected, combined with the supernatant, the supernatant was concentrated under reduced pressure at 50°C to 30% of the original volume to obtain a crude blueberry anthocyanin extract; The crude penicillin extract is added to a 60% ethanol solution with a mass concentration of 0.1% hydrochloric acid at a material-liquid ratio of 1:6, and passes thr...

Example Embodiment

[0020] Example 2

[0021] First, weigh 250g of blueberry fruit, wash and remove impurities, add 250mL of deionized water to the homogenizer for 13min, and add the blueberry slurry to 2.5L of 40% ethanol solution, and add 40g of fiber under a constant temperature water bath of 51℃. And use 200W ultrasonic ultrasound for 11 minutes, adjust the pH to 2.3 with a mass concentration of 10% hydrochloric acid solution, extract for 2.5 hours, place the extract in a centrifuge at 5000 / min and centrifuge for 13 minutes to obtain the precipitate and supernatant. The precipitate was extracted once again according to the above operation and the supernatant was collected, combined with the supernatant, the supernatant was concentrated to 33% of the original volume under reduced pressure at 53°C to obtain the crude blueberry anthocyanin extract; The crude blueberry anthocyanin extract was added to a 60% ethanol solution with a mass concentration of 0.1% hydrochloric acid at a material-to-liquid ...

Example Embodiment

[0023] Example 3

[0024] First, weigh 300g of blueberry fruit, wash and remove impurities, and add 300mL of deionized water to the homogenizer for 15 minutes to break and beating. Add 3L of the blueberry slurry to a 40% ethanol solution with mass concentration. Add 45g of cellulose in a constant temperature water bath at 52°C. Enzyme, and use 200W ultrasonic ultrasound for 12 minutes, adjust the pH to 2.5 with a mass concentration of 10% hydrochloric acid solution, extract for 3 hours, place the extract in a 6000 / min centrifuge and centrifuge for 15 minutes to obtain the precipitate and supernatant. The above operation is extracted once again and the supernatant is collected, the supernatant is combined, and the supernatant is concentrated to 35% of the original volume under reduced pressure at 55°C to obtain a crude blueberry anthocyanin extract; The crude penicillin extract is added to a 60% ethanol solution with a mass concentration of 0.1% hydrochloric acid at a material-liq...

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PUM

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Abstract

The invention discloses a method for extracting blueberry anthocyanin, and belongs to the field of natural organic chemistry. Blueberry fruits are adopted as raw materials, crushed and pulped, an ethanol solution with the mass fraction being 40% is adopted as an extraction solution, cellulase is added, and extraction is carried out under ultrasonic assistance; centrifugal separation is carried out, precipitation and repeated digestion are carried out once, extraction solutions are combined and concentrated, then the blueberry anthocyanin is promoted to be dissolved out through a high-voltage pulse electric field, and the dissolving-out amount of the blueberry anthocyanin of the extraction solutions is increased; then macroreticular resin adsorption, alcohol washing, concentration and freeze drying are carried out, and therefore the method for extracting the blueberry anthocyanin is obtained. A living example proves that operation is controllable, the method is unique, the production cost is reduced, pollution is avoided, the extraction rate of the prepared blueberry anthocyanin is as high as 80% or above, the purity reaches 75% or above, and higher medical care value and a wider practical application field are achieved.

Description

technical field [0001] The invention discloses a method for extracting blueberry anthocyanins, belonging to the field of natural organic chemistry. Background technique [0002] Anthocyanins, also known as anthocyanins, are a class of water-soluble natural pigments that widely exist in plants. Due to the instability of anthocyanins, anthocyanins mainly exist in plants. [0003] As a natural food coloring, anthocyanins are safe, non-toxic, rich in resources, and have certain nutritional and pharmacological effects. They have great application potential in food, cosmetics, and medicine. Anthocyanin is a pure natural anti-aging nutritional supplement. Studies have proved that it is the most effective antioxidant discovered by humans today, and it is the most effective antioxidant substance in nature. At the same time, studies have claimed that it can enhance vision, eliminate eye fatigue, and delay the aging of brain nerves. The anthocyanins contained in blueberries are curr...

Claims

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Application Information

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IPC IPC(8): C07D311/62C09B61/00
CPCC07D311/62C09B61/00
Inventor 仇颖莹张明王龙
Owner 仇颖莹
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