Method for extracting blueberry anthocyanin

Abstract

The invention discloses a method for extracting blueberry anthocyanin, and belongs to the field of natural organic chemistry. Blueberry fruits are adopted as raw materials, crushed and pulped, an ethanol solution with the mass fraction being 40% is adopted as an extraction solution, cellulase is added, and extraction is carried out under ultrasonic assistance; centrifugal separation is carried out, precipitation and repeated digestion are carried out once, extraction solutions are combined and concentrated, then the blueberry anthocyanin is promoted to be dissolved out through a high-voltage pulse electric field, and the dissolving-out amount of the blueberry anthocyanin of the extraction solutions is increased; then macroreticular resin adsorption, alcohol washing, concentration and freeze drying are carried out, and therefore the method for extracting the blueberry anthocyanin is obtained. A living example proves that operation is controllable, the method is unique, the production cost is reduced, pollution is avoided, the extraction rate of the prepared blueberry anthocyanin is as high as 80% or above, the purity reaches 75% or above, and higher medical care value and a wider practical application field are achieved.

Description

technical field [0001] The invention discloses a method for extracting blueberry anthocyanins, belonging to the field of natural organic chemistry. Background technique [0002] Anthocyanins, also known as anthocyanins, are a class of water-soluble natural pigments that widely exist in plants. Due to the instability of anthocyanins, anthocyanins mainly exist in plants. [0003] As a natural food coloring, anthocyanins are safe, non-toxic, rich in resources, and have certain nutritional and pharmacological effects. They have great application potential in food, cosmetics, and medicine. Anthocyanin is a pure natural anti-aging nutritional supplement. Studies have proved that it is the most effective antioxidant discovered by humans today, and it is the most effective antioxidant substance in nature. At the same time, studies have claimed that it can enhance vision, eliminate eye fatigue, and delay the aging of brain nerves. The anthocyanins contained in blueberries are curr...

AI Technical Summary

Problems solved by technology

[0006] The technical problem mainly solved by the present invention: In the process of extracting anthocyanins in the current traditional method, water extraction or solvent extraction will destroy the anthocyanin structure due to high temperature, affecting its biological activity and yield, while supercritical extraction Although the method can improve the extraction rate, the equipment is expensive, the cost is high, and the status quo that is not easy for industrialized production provides a kind of blueberry fruit as raw material, crushing and beating, using a mass fraction of 40% ethanol solution as the extraction solution, and adding cellulase , extracted with the assistance of ultrasound, and then centrifuged, precipitated and leached repeatedly once, and after the combined extract was concentrated, a short pulse of high voltage was applied to the material between the two electrodes through a high-voltage pulse electric field, so that the polar substances in the action of the electric field Move towards the electrode at a high speed, and use the principle of cell membrane electroporation to cause irreversible damage to the tissue cells, thereby promoting the dissolution of blueberry anthocyanins, increasing the dissolution of blueberry anthocyanins in the extract, and then adsorbed by macroporous adsorption resin and washed with alcohol , concentrated, freeze-dried, thereby obtaining blueberry anthocyanin extraction method