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Establishing method for jaboticaba tissue culture system

A tissue culture, garbo fruit technology, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problems of intolerance to storage, fruit damage, easy water loss of fresh fruit, etc., to prevent cardiovascular disease, increase The effect of resistance

Inactive Publication Date: 2016-11-16
李军
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, fresh fruit is easy to lose water, and it will ferment after 2-3 days at normal room temperature. It is not resistant to storage, which greatly limits the expansion of its fresh fruit market. Therefore, although the skin of Jiabao fruit is hard, care should be taken to avoid it during harvesting. Collide and cause wounds to avoid fruit damage, dehydration or infection of bacteria to accelerate rot

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] (1) Seed propagation: This is the most important method of propagation of Jiabao fruit at present. Each fruit has 1 to 4 seeds, which are irregularly shaped and polyembryonic. Fresh seeds germinate better. Generally, the peel is crushed and the seeds are squeezed out. After 1-2 days, the pulp is acidified and rotted, and then the pulp is washed with water before sowing. After the fruit is harvested, store it in a plastic bag and refrigerate it at 5-8°C. When you want to sow, take out the seeds and wash them before sowing. The refrigerated fruit can last for 1-2 months, and there is still a certain germination rate. The seeds germinate 20-40 days after sowing, and the germination is slower when the temperature is low.

[0019] (2) Grafting method: It takes 6-10 years for the seedlings of most varieties of Jiabao fruit to start to bear fruit. The grafting method can be used to bear fruit early, and it can bear fruit about 3 years after grafting. Select biennial seedlings ...

Embodiment 2

[0024] (1) Disinfection of explants: Take the stem tip of the robust Gerbera fruit that year, scrub it clean, soak it in washing powder solution for 6 minutes, and then gently scrub the surface of the explant with a soft brush to remove the dust and some bacteria on the surface. Remove it, rinse it with tap water for 1 hour, and place it in an ultra-clean workbench. Disinfect it with 75% ethanol for 6 seconds, wash it with sterile water for 4 times, then disinfect it with 0.1% mercury liter solution for 10 minutes, rinse it with sterile water for 5 times, and then wash it with sterile water for 10 minutes. Use bacterial filter paper to dry the water droplets on the surface before use.

[0025] (2) Start-up culture: Inoculate the shoot tips treated in step (1) into the start-up medium for bud induction. After inoculation, culture them in total darkness at 26°C for 5 days, and then the pollution rate is as low as 3%, and the survival rate reaches 78%. %. Then it was illuminated...

Embodiment 3

[0031] (1) Disinfection of explants: Take the stem tip of the healthy Gerbera fruit that year, scrub it clean, soak it in washing powder solution for 8 minutes, then gently scrub the surface of the explant with a soft brush to remove the dust and some bacteria on the surface Remove, then rinse with tap water for 3 hours, then in the ultra-clean workbench, first disinfect with 75% ethanol for 12 seconds, then wash with sterile water for 5 times, then disinfect with 0.1% mercury liter solution for 14 minutes, rinse with sterile water for 6 times, and then use sterile filter paper Wipe off any water droplets on the surface and set aside.

[0032] (2) Start-up culture: Inoculate the shoot tips treated in step (1) into the start-up medium for bud induction. After inoculation, culture them in full darkness at 28°C for 4 days, and then the pollution rate is as low as 5%, and the survival rate reaches 81.6% %. Then, it was illuminated for 13 hours every day and cultured for 29 days u...

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PUM

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Abstract

The invention discloses an establishing method for a jaboticaba tissue culture system and a method for quickly acquiring a large number of excellent jaboticaba variety test tube plantlets within a short period of time through a plant tissue culture technique. The method comprises the following steps: taking jaboticaba stem tip as an explant; disinfecting explant; performing initiation culture; performing enrichment culture; performing strong seedling culture; performing rooting culture; performing hardening-seedling and transplanting, thereby effectively increasing the inductivity of buds, subculture coefficient and rooting rate, establishing a complete jaboticaba tissue culture system and being convenient for performing industrialized production on jaboticaba seedlings. The fruit obtained according to the method provided by the invention can be used for making wine, can be prepared into jelly and jam, has a healthcare function to the human body, can prevent cardiovascular diseases, can promote immunity and can whiten skin.

Description

technical field [0001] The invention relates to the field of forest fruit trees, in particular to a method for establishing a jaboica tissue culture system. Background technique [0002] The unique characteristics of Jiabao fruit are evergreen all year round, slow growth, beautiful tree shape, and will not parasitize scale insects and aphids. The fruit is both edible and ornamental. It is very suitable for balcony potting, garden landscape or potted ornamental. The fruit can be eaten fresh, and can also be processed into fruit wine, fruit juice, ice cream and pastry fillings, etc. However, fresh fruit is easy to lose water, and it will ferment after 2-3 days at normal room temperature. It is not resistant to storage, which greatly limits the expansion of its fresh fruit market. Therefore, although the skin of Jiabao fruit is hard, care should be taken to avoid it during harvesting. Collide and cause wounds to avoid fruit damage, water loss or infection of germs to accelera...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/005A01H4/008
Inventor 李军
Owner 李军