Method for separating vitamin B12 and propionic acid from fermentation liquid
A technology for fermentation broth and vitamins is applied in the field of separating vitamin B12 and propionic acid, which can solve the problems of limited production efficiency and inability to obtain high-purity propionic acid, and achieve the effects of fast separation, improved production efficiency, and fast speed.
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Embodiment 1
[0033] With the preservation number of CCTCC No.M 207015 Propionibacterium freudenii NX-4, inoculated in the culture medium, the culture medium is: glucose 20g / L, yeast powder 5g / L, peptone 7g / L, NaCl 6g / L, pH = 6.9, cultured at 33°C for 36 hours to obtain seed liquid; the seed liquid was inserted into the fermentation medium according to the inoculum volume of 10% of the fermentation medium volume, the fermentation medium was glucose 50g / L, corn steep liquor 20g / L, K 2 HPO 4 4g / L, MgSO 4 1g / L, NaCl1g / L, pH=6.9, cultured at 35°C for 42h to obtain a fermentation broth, wherein the mass fraction of propionic acid is 10-50g / L, and the mass fraction of vitamin B12 contained in the bacterial cells in the fermentation broth is 10-50mg / L.
[0034] Passing the above-mentioned fermentation broth through an ultrafiltration membrane to obtain a filtrate A and a concentrated solution B, wherein the concentrated solution B is a bacterial enrichment solution, and the vitamin B12 is extra...
Embodiment 2
[0038] With the preservation number of CCTCC No.M 207015 Propionibacterium freudenii NX-4, inoculated in the culture medium, the culture medium is: glucose 20g / L, yeast powder 5g / L, peptone 7g / L, NaCl 6g / L, pH = 6.9, cultured at 33°C for 36 hours to obtain seed liquid; the seed liquid was inserted into the fermentation medium according to the inoculum volume of 10% of the fermentation medium volume, the fermentation medium was glucose 50g / L, corn steep liquor 20g / L, K 2 HPO 4 4g / L, MgSO 4 1g / L, NaCl1g / L, pH=6.9, cultured at 35°C for 42h to obtain a fermentation broth, wherein the mass fraction of propionic acid is 10-50g / L, and the mass fraction of vitamin B12 contained in the bacterial cells in the fermentation broth is 10-50mg / L.
[0039]The above-mentioned fermentation liquid is passed through an ultrafiltration membrane to obtain a filtrate A and a concentrated solution B, wherein the concentrated solution B is a bacterial enrichment solution, and vitamin B12 is extract...
Embodiment 3
[0043] With the preservation number of CCTCC No.M 207015 Propionibacterium freudenii NX-4, inoculated in the culture medium, the culture medium is: glucose 20g / L, yeast powder 5g / L, peptone 7g / L, NaCl 6g / L, pH = 6.9, cultured at 33°C for 36 hours to obtain seed liquid; the seed liquid was inserted into the fermentation medium according to the inoculum volume of 10% of the fermentation medium volume, the fermentation medium was glucose 50g / L, corn steep liquor 20g / L, K 2 HPO 4 4g / L, MgSO 4 1g / L, NaCl1g / L, pH=6.9, cultured at 35°C for 42h to obtain a fermentation broth, wherein the mass fraction of propionic acid is 10-50g / L, and the mass fraction of vitamin B12 contained in the bacterial cells in the fermentation broth is 10-50mg / L.
[0044] Passing the above-mentioned fermentation broth through an ultrafiltration membrane to obtain a filtrate A and a concentrated solution B, wherein the concentrated solution B is a bacterial enrichment solution, and the vitamin B12 is extra...
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