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Preparation method of microecological preparation promoting straw degradation

A technology for micro-ecological preparations and straws, which is applied in the directions of biochemical equipment and methods, microorganisms, microorganisms, etc., can solve the problems of single inoculum mixture, poor decomposing effect, and low decomposing temperature, and achieves improvement of farmland fertility and straw degradation. Efficiency improvement effect

Inactive Publication Date: 2016-11-16
雷春生
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The technical problem to be solved by the present invention is to solve the problems that the current straw decomposing agent has a single mixture of bacterial agents, less functional strains, low decomposing temperature, poor decomposing effect, long cycle, and can only be completely degraded after several years of underground degradation. In the present invention, firstly, the strains in the soil are used to cultivate them through nutrients, serotonin and ethyleneimine are used as mutagens, and cellulose is used as a mutagenesis guide to screen the strains for mutagenesis, and at the same time, microwave Carry out screening and cultivation, and mix with the strains in the biogas slurry, then carbonize the wheat straw, as a carrier matrix, to adsorb the strains that have been screened and cultured, so as to prepare the strains that are rich in strains, strong in functionality, good in decomposing effect, and short in cycle probiotics

Method used

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Examples

Experimental program
Comparison scheme
Effect test

example 1

[0018] In parts by weight, take 46 parts of peptone, 34 parts of agar, 15 parts of yeast extract, 16 parts of sodium chloride, 1.0 part of serotonin, 0.6 part of ethyleneimine and 38 parts of distilled water, stir them evenly and put them into an autoclaved Sterilize the fungus pot at 118°C for 20 minutes to obtain a screening induction medium; according to the solid-to-liquid ratio of 1:7, take the soil at 12 cm underground and mix it with a 5% glucose solution, let it stand for 20 minutes, and then put it in a centrifuge In the machine, centrifuge at 12000r / min for 10min, collect the supernatant, and inoculate the supernatant into the above-mentioned screening induction medium according to the inoculum size of 10%, then place it in a constant temperature incubator, set the temperature at 28°C, and cultivate 25h; during the above culture process, every 55min, add cellulose to the screening induction medium, the amount of cellulose added for the first time is 0.8% of the qualit...

example 2

[0021]In parts by weight, take 48 parts of peptone, 32 parts of agar, 10 parts of yeast extract, 8 parts of sodium chloride, 0.8 part of serotonin, 0.4 part of ethyleneimine and 36 parts of distilled water, stir them evenly and put them into an autoclaved Sterilize the fungus pot at 118°C for 30 minutes to obtain the screening induction medium; according to the solid-to-liquid ratio of 1:9, take the soil at 16 cm underground and mix it with a 5% glucose solution, let it stand for 25 minutes, and then put it in a centrifuge In the machine, centrifuge at 12000r / min for 15min, collect the supernatant, and inoculate the supernatant into the above-mentioned screening induction medium according to the inoculum size of 15%, then place it in a constant temperature incubator, set the temperature at 36°C, and cultivate 28h; during the above culture process, every 70min, add cellulose to the screening induction medium, the amount of cellulose added for the first time is 1.1% of the qualit...

example 3

[0024] In parts by weight, take 47 parts of peptone, 33 parts of agar, 12 parts of yeast extract, 12 parts of sodium chloride, 0.9 part of 5-hydroxytryptamine, 0.5 part of ethyleneimine and 37 parts of distilled water, stir them evenly and put them into an autoclaved Sterilize the fungus pot at 118°C for 25 minutes to obtain the screening induction medium; according to the solid-to-liquid ratio of 1:8, take the soil at 14 cm underground and mix it with a 5% glucose solution, let it stand for 22 minutes, and then put it in a centrifuge In the machine, centrifuge at 12000r / min for 12min, collect the supernatant, and inoculate the supernatant into the above-mentioned screening induction medium according to the inoculum size of 12%, then place it in a constant temperature incubator, set the temperature at 32°C, and cultivate 26h; during the above culture process, every 62min, add cellulose to the screening induction medium, the amount of cellulose added for the first time is 1.0% o...

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Abstract

The invention relates to a preparation method of a microecological preparation promoting straw degradation, and belongs to the technical field of crop straw treatment. For the situation that at present, a straw decomposition agent has the problems that fungicide mixing is single, few functional strains exist, the decomposition temperature is low, the decomposition effect is poor, teh cycle is long, and underground degradation can be thoroughly completed after several years, the microecological preparation is prepared. The preparation method comprises the steps that by means of strains in soil itself, cultivation is conducted through nutrients; 5-hydroxy tryptamine and ethylenimine serve as mutagenic agents, cellulose serves as a mutagenic guide material, screening mutagenesis is conducted on the strains, screening cultivation is conducted by being assisted with microwave, and the treated stains are mixed with strains in biogas slurry; carbonization is conducted on wheat straw, the carbonized wheat straw serves as a carrier substrate, and adsorption is conducted on the strains subjected to the screening cultivation. Therefore, the prepared microecological preparation is rich in strain, high in functionality, good in decomposition effect and short in cycle.

Description

technical field [0001] The invention relates to a preparation method of a micro-ecological agent for promoting straw degradation, and belongs to the technical field of crop straw treatment. Background technique [0002] Crop straw is one of the most abundant substances in the world. The output of crop straw in the world is about 2.941 billion tons, and the output of crop straw in my country is about 700 million tons per year, of which straw feed, straw energy, straw papermaking and building materials account for Two-thirds and one-third are discarded or incinerated. Crop straw is an important resource in the crop production system. It contains a variety of effective components that can be used. In addition to the vast majority of carbon, it also contains elements such as nitrogen, phosphorus, potassium, silicon, and calcium, as well as cellulose and semi-fibers. , lignin, protein, amino acid and other active ingredients. [0003] In recent years, due to the application ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/01C12N1/00C05F11/08
CPCC05F11/08C12N1/00C12N15/01Y02E50/30Y02W30/40
Inventor 雷春生宋奇
Owner 雷春生
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