Quality control method of Periplaneta americana freeze-dried powder

A quality control method, the technology of Periplaneta americana, applied in the direction of measuring devices, preparation of test samples, color/spectral characteristics measurement, etc., can solve the problems of serious side effects, over-treatment, deterioration of the patient's body condition, etc., and achieve quality assurance and curative effect

Inactive Publication Date: 2016-11-23
SOUTHWEST MEDICAL UNIVERISTY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Western medicine still mainly treats tumors with surgery, radiotherapy and chemotherapy. The toxic and side effects are serious and extensive, leading to the deterioration of the patient's body condition and the decline in the quality of life. Most patients give up treatment because they cannot bear it, and even die from overtreatment.

Method used

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  • Quality control method of Periplaneta americana freeze-dried powder
  • Quality control method of Periplaneta americana freeze-dried powder
  • Quality control method of Periplaneta americana freeze-dried powder

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] Example 1 Amino Acid Identification

[0047] Weigh 0.5g of Periplaneta americana freeze-dried powder, add 10ml of 70% ethanol, sonicate for 10min, filter, collect the filtrate, evaporate to dryness in a water bath, add 5ml of 70% ethanol to dissolve the residue, and use it as the test solution.

[0048] Take alanine, phenylalanine, and glycine reference substances respectively, accurately weigh them, put them in a brown measuring bottle, add 70% ethanol to make a 1mg / ml reference substance solution.

[0049] According to the thin-layer chromatography test, draw 1 μl of each of the above four solutions, spot them on the same silica gel G thin-layer plate, use n-butanol-formic acid-water (75:15:10) as the developing solvent, and develop after saturation for 30 minutes. Take it out, dry it, spray it with 0.2% ninhydrin ethanol solution, heat it at 105°C until the spots are clear, and inspect it under sunlight.

[0050] The result is as figure 1 As shown, among the figure...

Embodiment 2

[0051] Embodiment 2 Determination of polypeptide content

[0052] The main active ingredients in Periplaneta americana freeze-dried powder are polypeptides. According to the relevant regulations under the 2010 edition of "Chinese Pharmacopoeia", Appendix VA UV-visible spectrophotometry, refer to the literature and use bovine serum albumin as a reference substance to determine the total polypeptides content.

[0053] 2.1 Preparation of reference solution

[0054] Accurately weigh 51.80mg of bovine serum albumin reference substance (BSA), put it in a 100ml volumetric flask, add distilled water to dissolve to the mark, shake well, and prepare a reference substance solution containing 0.5180mg bovine serum albumin per 1ml.

[0055] 2.2 Selection of detection wavelength

[0056] Precisely measure the reference substance solution, put 1ml of the sample solution into a 10ml graduated test tube with stopper, add 5.0ml of alkaline copper reagent, mix well, quickly add 0.5ml of phenol...

Embodiment 3

[0111] Embodiment 3 Amino Acid Content Determination

[0112] In Periplaneta americana freeze-dried powder, amino acid is one of the active ingredients, according to the relevant regulations under the 2010 edition of "Chinese Pharmacopoeia" one appendix VA ultraviolet-visible spectrophotometry, with alanine as a reference substance to measure the total amino acid content.

[0113] 3.1 Preparation of reference solution

[0114] Accurately weigh 16.08mg of alanine reference substance, put it in a 100ml volumetric flask, add 10% isopropanol to dissolve, and dilute to the mark as mother liquor. Accurately measure 4ml, put it in a 50ml volumetric flask, and bring it to the mark with water to prepare a reference solution containing 12.86μg alanine per 1ml.

[0115] 3.2 Selection of detection wavelength

[0116] Accurately measure the reference substance solution and the sample solution in a 10ml graduated test tube with a stopper, add 0.1ml of freshly prepared 1% Vc solution, 3ml ...

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Abstract

The invention discloses a quality control method of Periplaneta americana freeze-dried powder. The quality control method comprises the steps of carrying out amino acid identification by virtue of a thin layer chromatography, carrying out content determination on amino acid and polypeptide by virtue of ultraviolet-visible spectrophotometry, and carrying out content determination on uracil, hypoxanthine and creatinine by virtue of a high performance liquid chromatography. According to the quality control method, the quality of the Periplaneta americana freeze-dried powder is detected and controlled from several aspects of the qualitative identification of amino acid, the content determination of amino acid, polypeptide and nucleoside components, the composition analysis of amino acid and the molecular weight determination of polypeptide. Powerful guarantee is provided for the quality of the Periplaneta americana freeze-dried powder, and the quality and the treatment effect of a later-period preparation taking the Periplaneta americana freeze-dried powder as an intermediate is guaranteed.

Description

technical field [0001] The invention belongs to the technical field of traditional Chinese medicines, and in particular relates to a quality control method for freeze-dried powder of Periplaneta americana. Background technique [0002] The American cockroach (Periplaneta americana (L.)) is the dry worm body of an insect belonging to the genus Periplaneta in the subclass Insecta Pterosae, Blattelaorder, Blattedae, also known as cockroach, stone ginger, slippery worm, steal oil woman, tea woman, The medicine was first recorded in "Shen Nong's Materia Medica", which is said to be "salty and cold. It is mainly used for blood stasis, hard symptoms, cold and heat, broken accumulation, sore throat, internal cold, and childless". It has the effects of reducing swelling and is used to treat children with malnutrition, boils, carbuncle, throat moth, unknown swelling poison, syphilis, poisonous snakes, centipede bites and other diseases. Contains various active ingredients such as pro...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/90G01N30/06G01N30/14G01N30/02G01N21/33G01N1/28
CPCG01N30/90G01N1/28G01N21/33G01N30/02G01N30/06G01N30/14G01N2030/027G01N2030/062G01N2030/146
Inventor 张丹刘明华余昕陈斯玮周洁孙琴傅超美
Owner SOUTHWEST MEDICAL UNIVERISTY
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