Conductive hydrogels for affinity sensing

A hydrogel and sensor technology, applied in the field of conductive hydrogel for affinity sensing, can solve problems such as poor mechanical properties

Inactive Publication Date: 2016-11-23
RGT UNIV OF CALIFORNIA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, it is characterized by inherently poor mechanical properties

Method used

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  • Conductive hydrogels for affinity sensing
  • Conductive hydrogels for affinity sensing
  • Conductive hydrogels for affinity sensing

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0133] Embodiment 1: Preparation of PEDOT-polyethylene glycol hydrogel

[0134] To develop immunosensors, we constructed a novel composite conductive hydrogel, which includes conductive carboxyl-functionalized PEDOT and high molecular weight PEG. Carboxyl functionalization provides bioconjugation capabilities. The biointerface was prepared by polymerizing PEG on top of miniaturized gold electrodes, followed by electropolymerization of PEDOT copolymers into porous PEG gels using aqueous microemulsions. The PEDOT / PEG copolymer is compositionally tunable and its deposition on an array of electrode surfaces is controllable. The COOH groups located on the conductive polymer chains were tailored with B-IFN-γ antibodies through active ester groups. Conductive hydrogel electrodes immobilized with antibodies were used for label-free electrochemical detection of B-IFN-γ in buffer and bovine plasma. Binding of B-IFN-γ to antibodies results in a decrease in electrochemical signal due...

Embodiment 2

[0143] Embodiment 2: preparation sensor

[0144]Gold and ITO patterned electrodes were fabricated using photolithography and wet etching methods as previously reported. Micropatterned glass slides (including photoresist on top of the gold surface) were treated with oxygen plasma for 10 min and treated in 0.05% (3-acryloyloxypropyl)trichlorosilane in toluene under a nitrogen atmosphere. solution for about 1 hour to obtain a self-assembled monolayer of silane in the glass zone. This process is described in more detail below.

[0145] Positive resist (S1813) was spin-coated (2000 rpm, 30 sec) onto a glass slide coated with a 15 nm chrome adhesion layer and a 100 nm gold layer, resulting in a thick layer of photoresist. The glass sheet coated with photoresist material was softened and baked on a hot plate at 115° C. for 1 minute, then placed in contact with a photomask, and exposed to a 365 nm ultraviolet light source. The bottom plate is then placed in a developer solution (...

Embodiment 3

[0148] Embodiment 3: pulmonary tuberculosis detection

[0149] Antibody (bovine-IFN-γ) functionalized PEDOT conductive hydrogel was placed in the electrochemical wells set in Example 2. Different concentrations of the target substance (recombinant interferon gamma) prepared in 1X PBS were introduced into the electrochemical wells and incubated for about 20 minutes. Record the cyclic voltammetry curve.

[0150] like Figure 8A As shown, each sensing electrode (antibody-functionalized conductive hydrogel on gold microelectrodes) was connected to a potentiostat through a gold contact pad. In this way, each sensing electrode is individually addressed by a potentiostat.

[0151] Gold electrodes with conductive gel on the sensing wafer were the working electrodes and were addressed by potentiostats, respectively. We used an external Ag / AgCl reference electrode and a platinum wire counter electrode. These were respectively immersed in the electrolyte solution in the electroche...

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Abstract

The present invention provides a sensor for detection of disease markers, and methods for detecting a disease marker, using a conductive hydrogel modified with a biorecognition element. In one embodiment, the present invention provides a sensor having a substrate; at least one electrode contacting the substrate; a nanoporous membrane covering the electrode; and a biorecognition element selected from the group consisting of a peptide, an antibody, an enzyme, and an aptamer, wherein the biorecognition clement is covalently bound to the electrode, or covalently bound to a PEDOT random copolymer embedded within the nanoporous membrane.

Description

[0001] Cross References to Related Applications [0002] This application claims priority to US Provisional Application No. 61 / 918,099, filed December 19, 2013, the entire contents of which are incorporated herein by reference. [0003] Statement Concerning Rights to Inventions Made Under Federally Sponsored Research and Development [0004] Not applicable. Background of the invention [0005] Conductive polymers hold great promise as electrode coatings in biomolecular electronics and offer high electrical conductivity. However, it is characterized by inherently poor mechanical properties. The incorporation of hydrogels with conductive polymers may serve to modulate and improve mechanical properties, and also provide an antifouling surface as well as a reservoir for water-soluble bioactive agents. Besides, blends of electroactive polymers and biocompatible hydrogels provide three-dimensional structures with enhanced sensitivity and higher immobilization of biomolecules, and...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/327
CPCB82Y30/00G01N27/3277C12Q1/002C12Q1/003G01N33/5438C08L65/00G01N27/3278G01N33/54353G01N33/545G01N33/6866G01N2333/35G01N2333/57G01N2800/26
Inventor A·瑞夫辛Z·马萨卢俞定穆权恩真刘颖申东植
Owner RGT UNIV OF CALIFORNIA
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