Method for preparing glucosamine hydrochloride through mushroom dregs for producing glutamic acid

A technology of glucosamine hydrochloride and glutamic acid, which is applied in the fields of food and chemical industry, can solve the problems of large types and dosage of reagents, complicated preparation process, and high preparation cost, and achieve the effects of less types of reagents, simple process methods, and reduced preparation costs

Active Publication Date: 2016-12-07
SHANDONG NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Jiang Xinying confirmed the existence of glucosamine hydrochloride in the residue of glutamic acid bacteria in the process of preparing muramic acid from the residue of glutamic acid. However, glucosamine hydrochloride is a by-product of the preparation of muramic acid, and the yield is low. , and the preparation process is complex, the types and amounts of reagents required are too large, and the preparation cost is too high

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Example 1: Glucosamine hydrochloride is prepared by utilizing the bacterial residue of glutamic acid production

[0028] Specific steps are as follows:

[0029] (1) Get 250g of glutamic acid wet bacteria residue (the water content is 50%, that is, the dry weight of the bacteria residue is 125g), add 95% (volume percentage) ethanol 600ml each time, reflux twice, and filter after each reflux 1h , and finally get the filter residue.

[0030] (2) Add water to the filter residue to 1000ml, sonicate for 0.5h under the conditions of 20kHz, power 700w, ultrasonic 2s intermittent 2s, and filter to obtain 400ml of filtrate. Repeat the above process for a total of 5 ultrasounds, and combine the filtrates for a total of 2000ml.

[0031] (3) The filtrate was evaporated in vacuo at 65° C. to remove moisture to obtain 16 g of dry matter.

[0032] (4) Add 32ml of water to the dry substance, then add 32ml of 12mol / L HCL, hydrolyze at room temperature (25°C) for 4 hours, add 160ml of ...

Embodiment 2

[0036] Example 2: Glucosamine hydrochloride prepared by utilizing the bacterium residue producing glutamic acid

[0037] Specific steps are as follows:

[0038] (1) Get 150g of glutamic acid wet bacteria residue (water content is 50%, and dry weight is 75g), add dehydrated ethanol 400ml each time, reflux 2 times, add dehydrated alcohol 400ml reflux 1h after filtering each time, filter residue Repeat the reflux once, and filter to obtain the filter residue.

[0039](2) Add water to the filter residue to 600ml, sonicate for 0.5h under the conditions of 20kHz, power 600w, ultrasonic 2s intermittent 2s, filter to obtain 240ml of filtrate, add water to the filter residue to 600ml, repeat the ultrasonic for 5 times in the same way, and combine the filtrates 5 times 1200ml in total.

[0040] (3) The filtrate was evaporated in vacuo at 65° C. to remove moisture to obtain 10 g of dry matter.

[0041] (4) Add 20ml of water to the dry matter, then add 20ml of 12mol / L HCL, hydrolyze at...

Embodiment 3

[0044] Example 3: Glucosamine hydrochloride prepared by utilizing the bacterium residue producing glutamic acid

[0045] Adjust the concentration of ethanol used for the reflux of the bacterium residue to 90%, and the power used by the ultrasound is adjusted to 800w. The rest of the operations are the same as in Example 1. The prepared dry matter is 17g. Add 34ml of water to the dry matter, and then add 34ml of 12mol / L HCL , after hydrolysis at room temperature for 4 hours, add 170ml of methanol and let it stand for 1 hour, the liquid crystallizes, and the supernatant is poured out.

[0046] The decanted supernatant was adjusted to pH=7 with NaOH, and a large amount of white precipitates appeared. A white precipitate was obtained by filtration. 50 ml of methanol was added to the white precipitate. Stir and decant the supernatant. Add 50 ml of methanol to the remaining precipitate, stir to dissolve the supernatant, and remove the precipitate. The secondary supernatants were...

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Abstract

The invention discloses a method for preparing glucosamine hydrochloride through mushroom dregs for producing glutamic acid. The method comprises the following steps that firstly, the wet glutamic acid mushroom dregs are taken, an ethanol solution with the volume being 2-3 times that of the glutamic acid mushroom dregs is added into the glutamic acid mushroom dregs, reflux extracting and filtering are carried out, and filter residues are obtained; secondly, water is added into the filter residues, ultrasonic extracting and filtering are carried out, and filtrate is obtained; thirdly, the filtrate is evaporated to dryness, a dry substance is obtained, water and hydrochloric acid are sequentially added into the dry substance, hydrolyzing is carried out for 3-5 hours under the condition that the temperature ranges from 15 DEG C to 30 DEG C, after hydrolyzing is completed, methyl alcohol is added, standing is carried out, and liquid supernatant is poured out; fourthly, the pH of the liquid supernatant is adjusted to 7-7.2, filtering is carried out, and white precipitate is obtained; methyl alcohol is added into the white precipitate, stirring is carried out for dissolving, methyl alcohol liquid supernatant is obtained, methyl alcohol is added into residual white precipitate, the operation is repeated multiple times, the methyl alcohol liquid supernatant is mixed and concentrated to be dry, and the glucosamine hydrochloride is obtained. The method for efficiently preparing glucosamine hydrochloride through the mushroom dregs for producing the glutamic acid is established for the first time, the number of kinds of needed reagents and the amount of the needed reagents are small, and the preparation cost of the glucosamine hydrochloride is reduced.

Description

technical field [0001] The invention relates to a method for preparing glucosamine hydrochloride by utilizing the fungus residue for producing glutamic acid, which belongs to the field of food and chemical industry. Background technique [0002] Glucosamine hydrochloride is also called glucosamine hydrochloride. It has important physiological functions for the human body, participates in liver and kidney detoxification, and plays an anti-inflammatory and liver-protecting role. It has good curative effect on the treatment of rheumatoid arthritis and gastric ulcer. It is the main raw material for the synthesis of antibiotics and anticancer drugs, and can also be used in chemicals, food, cosmetics and feed additives. The main active ingredients of amino sugar capsule health products currently on the market are glucosamine hydrochloride or glucosamine sulfate. [0003] As a fine chemical product, glucosamine hydrochloride has a high price and a large market demand. At present...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H5/06C07H1/06
CPCC07H1/06C07H5/06
Inventor 刘代成
Owner SHANDONG NORMAL UNIV
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