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Ternary microbial restoration agent for restoring concrete cracks, preparation method and application thereof

A technology for microbial repair and concrete, applied in the field of ternary microbial repair agent for repairing concrete cracks, can solve the problem of no bacteria-induced calcium carbonate and other problems, and achieve the effects of improving repair efficiency, no environmental pollution, and wide source of raw materials

Inactive Publication Date: 2016-12-21
SHENZHEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The inventor found through literature research that so far, there is no report on the use of oxygen-releasing agents to promote the formation of calcium carbonate induced by bacteria

Method used

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Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0031] In addition, the present invention also provides a preparation method of a ternary microbial restoration agent for repairing concrete cracks, comprising the steps of:

[0032] The oxygen release agent, spore powder, nutrients and acid-base regulator are (70%-95%) in mass ratio: (1%-10%): (5%-15%): (1%-10%) crushing and grinding for later use; preferably, the particle size of the grinding is 100 mesh.

[0033] Mix the ground material evenly and press it into tablets, and add 20-40 mg of mixed powder into each punch to make particles to obtain the ternary microbial restoration agent. Preferably, the diameter of the particles is 3-10 mm, The height is 2-10mm.

[0034] The preparation method of the ternary microbial repairing agent for repairing concrete cracks provided by the present invention comprises: pulverizing and grinding the oxygen-releasing agent, spore powder and nutrients according to a certain mass ratio, mixing the ground materials evenly and pressing them in...

Embodiment 1

[0037] Embodiment 1-calcium peroxide-spore-sodium lactate ternary microbial restoration agent preparation

[0038] 1) Preparation of spores. The bacterial strain Bacillussp.H4 disclosed in Shenzhen University patent application CN201410478149.0 is used, and the preservation number is CGMCC NO.9629. Strain H4 was streaked with an inoculation loop on an alkaline LB solid medium plate, and cultured upside down at 30°C for 16-20 hours. Pick the colony on the surface of the plate with a burning sterilized inoculation loop and inoculate it into the alkaline LB culture solution. The culture solution is filled in a 250ml triangular flask, and the volume of each bottle of culture solution is 50ml. Place the inoculated triangular flask at a constant temperature and shake Cultivate in an incubator at 30°C and 150rpm for 16-20 hours, then use a 10ml micropipette to draw 8ml of this fermentation broth containing a large number of bacteria, add MMN-1 sporulation-promoting culture fluid, an...

Embodiment 2

[0052] Example 2 Magnesium peroxide-spore-sodium lactate ternary microbial remediation agent has excellent mineralization ability

[0053] Operation method is with embodiment 1, only need will replace the calcium peroxide powder in embodiment 1 with magnesium peroxide powder. The experiment found that after culturing for 30 days, 1.28 mg of insoluble calcium formed on the inner wall of the glass test tube to which 1 piece of "magnesium peroxide-spore-sodium lactate" was added, while only 0.37 mg of insoluble calcium was formed on the inner wall of the glass test tube to which 1 piece of "spore-sodium lactate" was added. mg insoluble calcium. Oxygen released from the slow reaction of magnesium peroxide with water facilitates the mineralization of the bacteria, forming more insoluble calcium.

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Abstract

The invention provides a ternary microbial restoration agent for restoring concrete cracks. The characteristic that an oxygen release compound reacts with water to release oxygen is utilized, the dissolved oxygen content in a concrete crack water environment is slowly replenished, an aerobic respiration function of microorganisms is promoted, and then the synthesis of calcium carbonate is accelerated, and the restoration efficiency is improved. In addition, the invention provides a preparation method of the ternary microbial restoration agent for restoring the concrete cracks. The preparation method comprises the steps of smashing and grinding the oxygen release compound, conidial powder and a nutrient substance according to a certain mass ratio, uniformly mixing the ground materials, tabletting, manufacturing into particles, and obtaining the ternary microbial restoration agent. The preparation method is simple in process, wide in raw material source, and low in cost, and has no pollution to environment.

Description

technical field [0001] The invention relates to the technical field of concrete self-repair, in particular to a ternary microbial repair agent for repairing concrete cracks, a preparation method and an application thereof. Background technique [0002] Due to the comprehensive effects of physical, chemical and biological factors on reinforced concrete structures, micro-cracks will inevitably appear in almost all concrete buildings, making it easier for harmful substances such as water, oxygen, carbon dioxide and chlorides to enter, and eventually lead to the failure of steel bars inside the concrete. The corrosion of the structure makes the overall structure often fail to reach the expected life and is damaged, which leads to increasingly prominent problems such as repair and maintenance. The methods of concrete repair are mainly divided into three categories: chemical method, physical method and biological method. Traditional physical and chemical repair methods can effect...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C04B24/04
CPCC04B40/0039C04B2111/72C04B22/068C04B24/00C04B24/04
Inventor 邢锋邓旭张金龙刘冰韩宁旭潘雯郑晓纯王晖王钦良冯俊玲
Owner SHENZHEN UNIV
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