Silicon phthalocyanine functionalized TiO2 mesocrystal based aflatoxin photoelectrochemical detection method

An aflatoxin and photoelectrochemical technology, applied in the direction of material electrochemical variables, can solve the problems of large forbidden band width and low photoelectric conversion efficiency, and achieve increased photocurrent, excellent photocurrent response and stability, and excellent electrical conductivity. Effect

Inactive Publication Date: 2017-01-04
FUJIAN NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, TiO 2 The band gap is large and can only be excited by ultraviolet light, so the photoelectric conversion efficiency is low in the visible light region

Method used

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  • Silicon phthalocyanine functionalized TiO2 mesocrystal based aflatoxin photoelectrochemical detection method
  • Silicon phthalocyanine functionalized TiO2 mesocrystal based aflatoxin photoelectrochemical detection method
  • Silicon phthalocyanine functionalized TiO2 mesocrystal based aflatoxin photoelectrochemical detection method

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Experimental program
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Effect test

Embodiment 1

[0031] A functionalized TiO based on silicon phthalocyanine dye 2 The preparation method of the photoelectrochemical detection sensor of mesogen (such as figure 1 shown):

[0032] (1) Pretreatment of the glassy carbon electrode: the glassy carbon electrode is first mechanically polished and polished on the suede covered with alumina powder, washed with secondary water to remove the residual powder on the surface, and then moved into an ultrasonic water bath for cleaning until it is cleaned, and finally Wash thoroughly with ethanol, dilute acid and water;

[0033] (2) Apply 3 μL of carbon nanohorns (CNHs) with a concentration of 3 mg / mL evenly on the surface of a clean glassy carbon electrode, dry under infrared light and cool to room temperature;

[0034] (3) Drop 3 μL of 0.5wt% sodium carboxymethylcellulose (CMC) on the CNHs modified electrode for 40 minutes, modify 3 μL of carbon quantum dots (CQDs) on the modified electrode and test it under infrared light Dry, then cool...

Embodiment 2

[0038] Carbon quantum dots (CQDs) materials are prepared by the following method: first add 1g of 4L-cysteine ​​to a beaker, then transfer the beaker to an electric furnace, heat at 280ºC for 5 minutes, cool the beaker to room temperature, and Add ultrapure water into the beaker and centrifuge at 8000 rpm for 5 minutes to obtain the final product, and collect the supernatant by rotary evaporation. Electron emission scanning electron microscopy (SEM) images of CQDs, as Figure 2C As shown, it can be seen that the average diameter of CQDs is 2–3 nm. Such as Figure 2D Shown is the ultraviolet spectrum of CQDs, indicating that CQDs have a broad absorption peak at 300-600nm; photogenerated electrons and holes are easily recombined, which can effectively improve the sensitivity of PEC biosensors.

Embodiment 3

[0040] Quasi-octahedral TiO functionalized with dendrimer silicon phthalocyanine dyes (SiPcs) 2 Mesoscopic crystal (QOTM@SiPcs) bioprobes were prepared by the following method:

[0041] (1) Preparation of dipentyl carboxylic acid SiPcs: 0.20 g SiPcCl 2 , 0.14 g anhydrous K 2 CO 3 and 0.5 mL of hexanoic acid were placed in 30 ml of toluene solvent and refluxed at 110 ° C for 48 hours; then the reaction mixture was cooled to room temperature and the solvent was evaporated to dryness under reduced pressure, and the liquid product was purified with dichloromethane in a Soxhlet extractor After 24 hours, the extracted substance was recrystallized and rinsed with a mixture of methanol and deionized water with a volume ratio of 1:1, and then redissolved in dichloromethane; Figure 2B As shown, the UV spectrum of SiPcs, the B band is at 355nm, the characteristic peak of the dimer is at 604nm, and the Q band is at 678nm; as Figure 2B As shown in the inset, the fluorescence spectru...

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Abstract

The invention discloses a silicon phthalocyanine functionalized TiO2 mesocrystal based aflatoxin photoelectrochemical detection method. The method is characterized in that a novel carbon nanocomposite formed by carbon nanohorns and carbon quantum dots serves as a sensor support; dendritic phthalocyanine functionalized quasi-octahedral TiO2 mesocrystal is introduced as a bioprobe to realize high-sensitivity detection of aflatoxin. The novel carbon nanocomposite formed by CNHs and CQDs has an excellent photoelectric property; SiPcs functionalized QOTM is superior in photocurrent response and stability as compared with single QOTM. An immunosensor enables mutual competition between standard AFB1 and marked AFB1 in an AFB1 antibody specificity combination process, and target object concentration and photocurrent intensity are in linear relation in a range of 10<-6>-10<2>ng / ml. The method which is universal is provided for ultra-sensitive detection of micromolecular substances.

Description

technical field [0001] The invention belongs to the technical field of novel functional materials and biosensing detection, and specifically relates to a silicon phthalocyanine functionalized TiO 2 Mesogenic photoelectrochemical detection of aflatoxins. Background technique [0002] Mycotoxins are toxic secondary metabolites produced by fungi that are widely present in food and feedstuffs. Mycotoxins are harmful to human health and may induce gene mutations, produce immunosuppression or cause diseases such as liver cancer; aflatoxin B 1 (AFB 1 ) is the most common highly toxic mycotoxin and is listed in the Group I carcinogens proposed by the International Agency for Research on Cancer. The maximum content of aflatoxin in food in Europe does not exceed 2ng / g, and in China and the United States does not exceed 20ng / g; for monitoring AFB 1 The degree of contamination has been extensively studied by electrochemical methods, enzyme-linked immunosorbent assay (ELISA), UHPLC-M...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/30
CPCG01N27/30
Inventor 戴宏刘楠囡高利红林燕语
Owner FUJIAN NORMAL UNIV
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