A kind of method that utilizes microalgae to prepare biodiesel

A biodiesel and microalgae technology, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of low biodiesel yield, low catalytic efficiency, long reaction time, etc., and achieve strong environmental adaptability, Improved catalytic efficiency and large reaction interface

Active Publication Date: 2018-08-24
黄艳红
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The existing biodiesel production process generally has the following problems: low catalytic efficiency, low biodiesel yield, long reaction time, corrosion equipment, complex process, high energy consumption, high cost, etc.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Preparation of medium for Haematococcus pluvialis: glucose 18mg / L, NaNO 3 1.0mg / L, KH 2 PO 4 1.2mg / L, chitosan 0.5mg / L, biogas slurry 7mg / L, f2 vitamin 1mL and seawater 1L;

[0027] A method for preparing biodiesel from microalgae, comprising the following steps:

[0028] S1. Cultivation of Haematococcus pluvialis: use Haematococcus pluvialis as raw material, inoculate it into the culture medium at an inoculation density of 5 million cells / mL, and irradiate it with red and blue dual-color LED supplementary light for 8h / day, among which the red light The ratio with blue light is 1:1, the light intensity is controlled at 2500LX, the culture temperature is controlled at 25°C, cultured for 8 days, and Haematococcus pluvialis cells are collected;

[0029] S2. Grease extraction: After the cells of Haematococcus pluvialls collected in step S1 are naturally air-dried, the wall of Haematococcus pluvialls is broken by ultrasonic wave, the ultrasonic power is controlled at 150...

Embodiment 2

[0034] Preparation of medium for Haematococcus pluvialis: glucose 15mg / L, NaNO 3 1.2mg / L, KH 2 PO 4 1.0mg / L, chitosan 0.8mg / L, biogas slurry 5mg / L, f2 vitamin 1.5mL and seawater 1L;

[0035] A method for preparing biodiesel from microalgae, comprising the following steps:

[0036]S1. Cultivation of Haematococcus pluvialis: use Haematococcus pluvialis as raw material, inoculate it into the culture medium at an inoculation density of 4.5 million cells / mL, and irradiate it with red and blue two-color LED supplementary light for 10h / day, among which the red light The ratio with blue light is 1:2, the light intensity is controlled at 2000LX, the culture temperature is controlled at 24°C, cultured for 8 days, and Haematococcus pluvialis cells are collected;

[0037] S2. Grease extraction: After the cells of Haematococcus pluvialls collected in step S1 are naturally air-dried, the wall of Haematococcus pluvialls is broken by ultrasonic wave, the ultrasonic power is controlled at ...

Embodiment 3

[0042] Preparation of medium for Haematococcus pluvialis: glucose 20mg / L, NaNO 3 1.0mg / L, KH 2 PO 4 1.0mg / L, chitosan 0.8mg / L, biogas slurry 7mg / L, f2 vitamin 2.0mL and seawater 1L;

[0043] A method for preparing biodiesel from microalgae, comprising the following steps:

[0044] S1. Cultivation of Haematococcus pluvialis: use Haematococcus pluvialis as raw material, inoculate it into the culture medium at an inoculation density of 4 million cells / mL, and irradiate it with red and blue two-color LED supplementary light for 10h / day, among which the red light The ratio with blue light is 1:2, the light intensity is controlled at 2500LX, the culture temperature is controlled at 23°C, cultured for 7 days, and Haematococcus pluvialis cells are collected;

[0045] S2. Grease extraction: After the cells of Haematococcus pluvialls collected in step S1 were naturally air-dried, ultrasonic waves were used to break the walls of Haematococcus pluvialls, the ultrasonic power was contr...

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Abstract

The invention discloses a method for preparing biodiesel from microalgae. The method comprises steps as follows: a culture medium is inoculated with haematococcus pluvialis, the haematococcus pluvialis is irradiated with a red and blue LED light-compensating lamp for 8-10 h every day and cultured at 23-25 DEG C for 7-8 d, and haematococcus pluvialis cells can be collected; the collected haematococcus pluvialis cells are subjected to wall breaking treatment with ultrasonic waves, extraction is performed with an organic solvent, microalgae oil is obtained, the microalgae oil, methanol and a zeolite molecular sieve catalyst are added to a reactor in the weight ratio being 1:(3-5):(0.01-0.03) for an esterification reaction, an esterification product is obtained, separated and purified, and the biodiesel can be obtained. According to the method, the haematococcus pluvialis which is short in production cycle, wide in application range, high in photosynthesis and high in oil content is taken as a raw material, and the problem of vegetable oil raw materials in the biodiesel development process is solved. Meanwhile, the method has the advantages that the preparation process is simple, the catalytic efficiency is high, the oil extraction rate is high, the product yield is high and the like, and a good foundation is laid for industrialization and mass production of biodiesel.

Description

technical field [0001] The invention belongs to the technical field of biomass energy, in particular to a method for preparing biodiesel from microalgae. Background technique [0002] With the increasing depletion of fossil fuels and the sharp rise in oil prices, the search for renewable energy has become a solution to human survival and to maintain the sustainable development of the world economy. As a new alternative energy, biodiesel is developing rapidly in countries all over the world. Biodiesel refers to oil crops such as soybeans, rapeseed, cotton, palm, etc., wild oil plants and engineering microalgae and other aquatic vegetable oils, animal fats, and catering waste oils, etc. Renewable diesel fuel to replace petrodiesel. The advantages of biodiesel are not limited to renewability. Compared with the existing diesel, it also has the following advantages: high cetane number, which makes the combustion performance better; better wetting performance, which makes the fue...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P7/64C12R1/89C10L1/02C11B1/02C11C3/10
CPCC10L1/02C11B1/02C11B1/10C11C3/10Y02E50/10
Inventor 黄健峻其他发明人请求不公开姓名
Owner 黄艳红
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